PMID- 36906248
OWN - NLM
STAT- MEDLINE
DCOM- 20230908
LR  - 20230908
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 268
DP  - 2023 Jun
TI  - Alteration of antioxidant status, glucose metabolism, and hypoxia signal pathway 
      in Eirocheir sinensis after acute hypoxic stress and reoxygenation.
PG  - 109604
LID - S1532-0456(23)00059-5 [pii]
LID - 10.1016/j.cbpc.2023.109604 [doi]
AB  - Dissolved oxygen (DO) is crucial for the survival of Chinese mitten crab 
      (Eirocheir sinensis); low DO levels adversely affect the health of these crabs. 
      In this study, we evaluated the underlying response mechanism of E. sinensis to 
      acute hypoxic stress by analyzing antioxidant parameters, glycolytic indicators, 
      and hypoxia signaling factors. The crabs were exposed to hypoxia for 0, 3, 6, 12, 
      and 24 h and reoxygenated for 1, 3, 6, 12, and 24 h. The hepatopancreas, muscle, 
      gill, and hemolymph were sampled at different exposure times to detect the 
      biochemical parameters and gene expression. The results showed that the activity 
      of catalase, antioxidants, and malondialdehyde in tissues significantly increased 
      under acute hypoxia and gradually decreased during the reoxygenation phase. Under 
      acute hypoxic stress, glycolysis indices, including hexokinase (HK), 
      phosphofructokinase, pyruvate kinase (PK), pyruvic acid (PA), lactate 
      dehydrogenase (LDH), lactic acid (LA), succinate dehydrogenase (SDH), glucose, 
      and glycogen in the hepatopancreas, hemolymph, and gills increased to varying 
      degrees but recovered to the control levels after reoxygenation. Gene expression 
      data showed that hypoxia signaling pathway-related genes, including 
      hypoxia-inducible factor-1alpha/beta (HIF1alpha/beta), prolyl hydroxylase (PHD), factor 
      inhibiting hypoxia-inducible factor (FIH), and glycolysis-related factors (HK and 
      PK) were upregulated, showing that the HIF signaling pathway was activated under 
      hypoxic conditions. In conclusion, acute hypoxic exposure activated the 
      antioxidant defense system, glycolysis, and HIF pathway to respond to adverse 
      conditions. These data contribute to elucidating the defense and adaptive 
      mechanisms of crustaceans to acute hypoxic stress and reoxygenation.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Chen, Xue
AU  - Chen X
AD  - College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China.
FAU - Feng, Wenrong
AU  - Feng W
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China. Electronic address: 
      fengwenrong@ffrc.cn.
FAU - Yan, Fengyuan
AU  - Yan F
AD  - College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China.
FAU - Li, Wenjing
AU  - Li W
AD  - Jiangsu Haorun Biological Industry Group Co., Ltd, Taizhou 225300, China; Jiangsu 
      Haorun National Crab Seed Technology Co., Ltd, Taizhou 225300, China.
FAU - Xu, Pao
AU  - Xu P
AD  - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China.
FAU - Tang, Yongkai
AU  - Tang Y
AD  - College of Fisheries and Life, Shanghai Ocean University, Shanghai 201306, China; 
      Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, 
      Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, 
      Chinese Academy of Fishery Sciences, Wuxi 214081, China. Electronic address: 
      tangyk@ffrc.cn.
LA  - eng
PT  - Journal Article
DEP - 20230310
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Antioxidants)
RN  - IY9XDZ35W2 (Glucose)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - *Antioxidants/metabolism
MH  - Carbohydrate Metabolism
MH  - *Glucose/metabolism
MH  - *Hypoxia/metabolism
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism
MH  - Oxygen/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - Antioxidative response
OT  - Eirocheir sinensis
OT  - Glycolysis
OT  - Hypoxia signaling pathway
OT  - Hypoxic stress
COIS- Declaration of competing interest All authors disclosed no conflict of interest.
EDAT- 2023/03/12 06:00
MHDA- 2023/04/11 06:41
CRDT- 2023/03/11 19:32
PHST- 2022/10/25 00:00 [received]
PHST- 2023/02/20 00:00 [revised]
PHST- 2023/03/06 00:00 [accepted]
PHST- 2023/04/11 06:41 [medline]
PHST- 2023/03/12 06:00 [pubmed]
PHST- 2023/03/11 19:32 [entrez]
AID - S1532-0456(23)00059-5 [pii]
AID - 10.1016/j.cbpc.2023.109604 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2023 Jun;268:109604. doi: 
      10.1016/j.cbpc.2023.109604. Epub 2023 Mar 10.