PMID- 36906987
OWN - NLM
STAT- MEDLINE
DCOM- 20230403
LR  - 20230413
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 156
DP  - 2023 Apr
TI  - BMAL1 regulates MUC1 overexpression in ovalbumin-induced asthma.
PG  - 77-84
LID - S0161-5890(23)00047-0 [pii]
LID - 10.1016/j.molimm.2023.03.004 [doi]
AB  - Asthma often presents with a daily rhythm; however, the underlying mechanisms 
      remain unclear. Circadian rhythm genes have been proposed to regulate 
      inflammation and mucin expression. Here, ovalbumin (OVA)-induced mice and serum 
      shock human bronchial epidermal cells (16HBE) were used in in vivo and in vitro 
      models, respectively. We constructed a brain and muscle ARNT-like 1 (BMAL1) 
      knockdown 16HBE cell line to analyze the effects of rhythmic fluctuations on 
      mucin expression. Serum immunoglobulin E (IgE) and circadian rhythm genes in 
      asthmatic mice showed rhythmic fluctuation amplitude. Mucin (MUC) 1 and MUC5AC 
      expression was increased in the lung tissue of the asthmatic mice. MUC1 
      expression was negatively correlated with that of the circadian rhythm genes, 
      particularly BMAL1 (r = -0.546, P = 0.006). There was also a negative correlation 
      between BMAL1 and MUC1 expression (r = -0.507, P = 0.002) in the serum shock 
      16HBE cells. BMAL1 knockdown negated the rhythmic fluctuation amplitude of MUC1 
      expression and upregulated MUC1 expression in the 16HBE cells. These results 
      indicate that the key circadian rhythm gene, BMAL1, causes periodic changes in 
      airway MUC1 expression in OVA-induced asthmatic mice. Targeting BMAL1 to regulate 
      periodic changes in MUC1 expression may, therefore, improve asthma treatments.
CI  - Copyright (c) 2023 Elsevier Ltd. All rights reserved.
FAU - Tang, Lingling
AU  - Tang L
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province 
      Hospital of Chinese Medicine, Nanjing, Jiangsu 210004, China.
FAU - Zhang, Xiaona
AU  - Zhang X
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province 
      Hospital of Chinese Medicine, Nanjing, Jiangsu 210004, China.
FAU - Xu, Yanqiu
AU  - Xu Y
AD  - Nanjing Jiangning Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu 
      211100, China.
FAU - Liu, Li
AU  - Liu L
AD  - Department of Central Lab, Affiliated Hospital of Nanjing University of Chinese 
      Medicine, Nanjing, Jiangsu 210029, China.
FAU - Sun, Xianhong
AU  - Sun X
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province 
      Hospital of Chinese Medicine, Nanjing, Jiangsu 210004, China.
FAU - Wang, Bohan
AU  - Wang B
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province 
      Hospital of Chinese Medicine, Nanjing, Jiangsu 210004, China.
FAU - Yu, Keyao
AU  - Yu K
AD  - Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province 
      Hospital of Chinese Medicine, Nanjing, Jiangsu 210004, China.
FAU - Zhang, Hui
AU  - Zhang H
AD  - Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese 
      Medicine, Nanjing, Jiangsu 210022, China.
FAU - Zhao, Xia
AU  - Zhao X
AD  - Jiangsu Key Laboratory of Pediatric Respiratory Disease, Institute of Pediatrics, 
      Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 
      210023, China. Electronic address: Zhaoxiahy@njucm.edu.cn.
FAU - Wang, Xiongbiao
AU  - Wang X
AD  - Department of Respiratory Medicine, Putuo Hospital, Shanghai University of 
      Traditional Chinese Medicine, Shanghai 200062, China. Electronic address: 
      xiongbiao2004@shutcm.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230310
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (ARNTL Transcription Factors)
RN  - 0 (MUC1 protein, human)
RN  - 0 (Mucin-1)
RN  - 0 (Mucins)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Mice
MH  - *ARNTL Transcription Factors/genetics/metabolism
MH  - *Asthma/chemically induced/genetics/metabolism
MH  - Brain/metabolism
MH  - Mucin-1/genetics/metabolism
MH  - Mucins/metabolism
MH  - Muscles/metabolism
MH  - Ovalbumin/metabolism
OTO - NOTNLM
OT  - Airway mucus
OT  - Asthma
OT  - BMAL1
OT  - MUC1
OT  - Rhythm
EDAT- 2023/03/13 06:00
MHDA- 2023/03/31 06:42
CRDT- 2023/03/12 19:05
PHST- 2022/10/14 00:00 [received]
PHST- 2023/02/20 00:00 [revised]
PHST- 2023/03/02 00:00 [accepted]
PHST- 2023/03/31 06:42 [medline]
PHST- 2023/03/13 06:00 [pubmed]
PHST- 2023/03/12 19:05 [entrez]
AID - S0161-5890(23)00047-0 [pii]
AID - 10.1016/j.molimm.2023.03.004 [doi]
PST - ppublish
SO  - Mol Immunol. 2023 Apr;156:77-84. doi: 10.1016/j.molimm.2023.03.004. Epub 2023 Mar 
      10.