PMID- 36908538 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230314 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) VI - 13 IP - 11 DP - 2023 Mar 1 TI - Combined theoretical and experimental insights on DNA and BSA binding interactions of Cu(ii) and Ni(ii) complexes along with the DPPH method of antioxidant assay and cytotoxicity studies. PG - 7632-7644 LID - 10.1039/d2ra08341h [doi] AB - This present study delineates the syntheses, detailed characterization and anti-proliferative potential against SiHa (cervical cancer cell) of two mononuclear complexes of Cu(ii) and Ni(ii) using a Schiff base ligand (L) derived from 2-hydroxybenzaldehyde and N-methyl-propane 1,3-diamine. The crystallographic results show the centro-symmetric space group of orthorhombic nature (Pccn) for Cu(ii) complex (1) where the central Cu(ii) has an inversion center symmetry with six co-ordinations resulting in a distorted octahedral geometry. Whereas, in complex (2), the two independent Ni(ii) atoms present in the special position within version symmetry and form a distorted geometry of octahedral nature with six coordinations. Absorption spectral titrations with Calf Thymus (CT) DNA and the extent of the decrease in relative emission intensities of DNA-bound ethidium bromide (EB) upon adding the complexes reveal the parallel trend in DNA binding affinities for both the complexes but with a small extent of binding capabilities. Bovine serum albumin (BSA) interaction studies demonstrate that complex 1 exhibits more promiscuous binding with BSA as compared to complex 2 from the spectroscopic and theoretical approaches. alpha,alpha-Diphenyl-beta-picrylhydrazyl (DPPH) free radical scavenging method shows a little antioxidant or free radical scavenging activity for both the studied complexes. Cytotoxicity studies against SiHa expressed that the percentage of cell viability was reduced with time whereas in the same concentration and conditions, the viability percentage was higher for 3T3-L1 (several normal cell lines of mouse). The fluorescence imaging obtained from acridine orange (AO) and ethidium bromide (EtBr) demonstrates that the colour of the cancer cells has changed gradually dictating the cell apoptosis from day 1 to day 3. CI - This journal is (c) The Royal Society of Chemistry. FAU - Acharya, Prasun AU - Acharya P AD - Department of Chemistry Mugberia Gangadhar Mahavidyalaya Bhupatinagar Purba Medinipur-721425 West Bengal India bsmgm1977@gmail.com bidhansamanta@yahoo.in +91-3220-270236. FAU - Kuila, Arun AU - Kuila A AD - Department of Chemistry Mugberia Gangadhar Mahavidyalaya Bhupatinagar Purba Medinipur-721425 West Bengal India bsmgm1977@gmail.com bidhansamanta@yahoo.in +91-3220-270236. FAU - Pramanik, Ushasi AU - Pramanik U AD - Department of Chemistry, IISER Bhopal Bhopal Bypass Road, Bhauri Bhopal 462 066 Madhya Pradesh India. FAU - Hathwar, Venkatesha R AU - Hathwar VR AD - School of Physical and Applied Sciences, Goa University Taleigao Plateau Goa 403 206 India. FAU - Brandao, Paula AU - Brandao P AUID- ORCID: 0000-0002-4746-6073 AD - Departamento de Quimica, CICECO, Universidade de Aveiro 3810-193 Aveiro Portugal. FAU - Mukherjee, Saptarshi AU - Mukherjee S AUID- ORCID: 0000-0001-8280-0754 AD - Department of Chemistry, IISER Bhopal Bhopal Bypass Road, Bhauri Bhopal 462 066 Madhya Pradesh India. FAU - Maity, Swapan AU - Maity S AD - School of Materials Science and Technology (SMST), Indian Institute of Technology (IIT), BHU India. FAU - Maity, Tithi AU - Maity T AUID- ORCID: 0000-0002-1256-399X AD - Department of Chemistry, Prabhat Kumar College Purba Medinipur-721401 Contai West Bengal India. FAU - Maity, Ribhu AU - Maity R AD - Department of Chemistry Mugberia Gangadhar Mahavidyalaya Bhupatinagar Purba Medinipur-721425 West Bengal India bsmgm1977@gmail.com bidhansamanta@yahoo.in +91-3220-270236. FAU - Chandra Samanta, Bidhan AU - Chandra Samanta B AUID- ORCID: 0000-0002-6029-371X AD - Department of Chemistry Mugberia Gangadhar Mahavidyalaya Bhupatinagar Purba Medinipur-721425 West Bengal India bsmgm1977@gmail.com bidhansamanta@yahoo.in +91-3220-270236. LA - eng PT - Journal Article DEP - 20230308 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC9993069 COIS- It is hereby confirmed that no conflicts of interest to be declared. EDAT- 2023/03/14 06:00 MHDA- 2023/03/14 06:01 PMCR- 2023/03/08 CRDT- 2023/03/13 03:47 PHST- 2022/12/31 00:00 [received] PHST- 2023/02/21 00:00 [accepted] PHST- 2023/03/13 03:47 [entrez] PHST- 2023/03/14 06:00 [pubmed] PHST- 2023/03/14 06:01 [medline] PHST- 2023/03/08 00:00 [pmc-release] AID - d2ra08341h [pii] AID - 10.1039/d2ra08341h [doi] PST - epublish SO - RSC Adv. 2023 Mar 8;13(11):7632-7644. doi: 10.1039/d2ra08341h. eCollection 2023 Mar 1.