PMID- 36911207
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20230424
LR  - 20230503
IS  - 2452-199X (Electronic)
IS  - 2452-199X (Linking)
VI  - 26
DP  - 2023 Aug
TI  - Macrophage-derived exosomes modulate wear particle-induced osteolysis via 
      miR-3470b targeting TAB3/NF-kappaB signaling.
PG  - 181-193
LID - 10.1016/j.bioactmat.2023.02.028 [doi]
AB  - Aseptic prosthesis loosening (APL) is one of the most prevalent complications 
      associated with arthroplasty. The main cause is the periprosthetic osteolysis 
      induced by wear particles. However, the specific mechanisms of crosstalk between 
      immune cells and osteoclasts/osteoblasts during osteolysis are unclear. In this 
      study, we report the role and mechanism of macrophage-derived exosomes in wear 
      particle-induced osteolysis. The results of exosomes up-taken experiments 
      revealed that osteoblast and mature osteoclasts capture macrophage-derived 
      exosomes (M-Exo). Next-generation sequencing and RT-qPCR on M-Exo revealed that 
      exosomal microRNA miR-3470b was downregulated in wear particle-induced 
      osteolysis. The results of analysis on Luciferase reporter assays/fluorescence in 
      situ hybridization (FISH)/immunofluorescence (IF)/immunohistochemistry (IHC) and 
      co-culture experiments demonstrated that wear particles induced osteoclast 
      differentiation by increasing the expression of NFatc1 via M-Exo miR-3470b 
      targeting TAB3/ NF-kappaB signaling. We also illustrate that engineered exosomes 
      enriching miR-3470b facilitated to suppressed the osteolysis; the 
      microenvironment enriching with miR-3470b could suppress wear particle-induced 
      osteolysis via inhibition of TAB3/ NF-kappaB in vivo. In summary, our findings 
      indicate that macrophage-derived exosomes transfer to osteoclasts to induce 
      osteolysis in wear particle-induced APL. Engineering exosomes enriching with 
      miR-3470b might be a novel strategy for the targeting treatment of bone 
      resorption-related diseases.
CI  - (c) 2023 The Authors.
FAU - Pan, Baiqi
AU  - Pan B
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
FAU - Zhang, Ziji
AU  - Zhang Z
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
FAU - Wu, Xiaoyu
AU  - Wu X
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
FAU - Xian, Guoyan
AU  - Xian G
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
AD  - Universite de Paris, CNRS, INSERM, B3OA, Paris, France.
FAU - Hu, Xuantao
AU  - Hu X
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
FAU - Gu, Minghui
AU  - Gu M
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
FAU - Zheng, Linli
AU  - Zheng L
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
FAU - Li, Xiang
AU  - Li X
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
AD  - Department of Spine Surgery, The first affiliated hospital of Sun Yat-sen 
      University, China.
FAU - Long, Lingli
AU  - Long L
AD  - Research Center of Translational Medicine, The First Affiliated Hospital of Sun 
      Yat-sen University, China.
FAU - Chen, Weishen
AU  - Chen W
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
FAU - Sheng, Puyi
AU  - Sheng P
AD  - Department of Joint Surgery, The First Affiliated Hospital of Sun Yat-sen 
      University, China.
AD  - Guangdong Provincial Key Laboratory of Orthopaedics and Traumatology, The First 
      Affiliated Hospital of Sun Yat-Sen University, China.
LA  - eng
PT  - Journal Article
DEP - 20230303
PL  - China
TA  - Bioact Mater
JT  - Bioactive materials
JID - 101685294
PMC - PMC9999169
OTO - NOTNLM
OT  - Aseptic prothesis loosening
OT  - Exosome
OT  - Inflammatory osteolysis
OT  - Macrophage
OT  - Non-coding RNA
COIS- No potential conflict of interest was reported by the authors.
EDAT- 2023/03/14 06:00
MHDA- 2023/03/14 06:01
PMCR- 2023/03/03
CRDT- 2023/03/13 04:22
PHST- 2022/12/06 00:00 [received]
PHST- 2023/02/25 00:00 [revised]
PHST- 2023/02/25 00:00 [accepted]
PHST- 2023/03/14 06:00 [pubmed]
PHST- 2023/03/14 06:01 [medline]
PHST- 2023/03/13 04:22 [entrez]
PHST- 2023/03/03 00:00 [pmc-release]
AID - S2452-199X(23)00073-7 [pii]
AID - 10.1016/j.bioactmat.2023.02.028 [doi]
PST - epublish
SO  - Bioact Mater. 2023 Mar 3;26:181-193. doi: 10.1016/j.bioactmat.2023.02.028. 
      eCollection 2023 Aug.