PMID- 36915643 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230315 IS - 2197-4365 (Print) IS - 2197-4365 (Electronic) IS - 2197-4365 (Linking) VI - 10 IP - 1 DP - 2023 TI - Large-scale expansion of human umbilical cord-derived mesenchymal stem cells using PLGA@PLL scaffold. PG - 18 LID - 10.1186/s40643-023-00635-6 [doi] LID - 18 AB - Mesenchymal stem cells (MSCs) are highly important in biomedicine and hold great potential in clinical treatment for various diseases. In recent years, the capabilities of MSCs have been under extensive investigation for practical application. Regarding therapy, the efficacy usually depends on the amount of MSCs. Nevertheless, the yield of MSCs is still limited due to the traditional cultural methods. Herein, we proposed a three-dimensional (3D) scaffold prepared using poly lactic-co-glycolic acid (PLGA) nanofiber with polylysine (PLL) grafting, to promote the growth and proliferation of MSCs derived from the human umbilical cord (hUC-MSCs). We found that the inoculated hUC-MSCs adhered efficiently to the PLGA scaffold with good affinity, fast growth rate, and good multipotency. The harvested cells were ideally distributed on the scaffold and we were able to gain a larger yield than the traditional culturing methods under the same condition. Thus, our cell seeding with a 3D scaffold could serve as a promising strategy for cell proliferation in the large-scale production of MSCs. Moreover, the simplicity and low preparation cost allow this 3D scaffold to extend its potential application beyond cell culture. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40643-023-00635-6. CI - (c) The Author(s) 2023. FAU - Liu, Yujie AU - Liu Y AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - Guangzhou Junyuankang Biotechnology Co., Ltd., Guangzhou, 510530 China. FAU - Amissah, Obed Boadi AU - Amissah OB AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - University of Chinese Academy of Sciences, Beijing, 100049 China. GRID: grid.410726.6. ISNI: 0000 0004 1797 8419 FAU - Huangfang, Xiaoying AU - Huangfang X AD - School of Medicine, Guilin Medical University, Guilin, 541199 China. GRID: grid.443385.d. ISNI: 0000 0004 1798 9548 FAU - Wang, Ling AU - Wang L AD - School of Life Sciences, University of Science and Technology of China, Hefei, 230027 China. GRID: grid.59053.3a. ISNI: 0000000121679639 FAU - Dieu Habimana, Jean de AU - Dieu Habimana J AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - University of Chinese Academy of Sciences, Beijing, 100049 China. GRID: grid.410726.6. ISNI: 0000 0004 1797 8419 FAU - Lv, Linshuang AU - Lv L AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - University of Chinese Academy of Sciences, Beijing, 100049 China. GRID: grid.410726.6. ISNI: 0000 0004 1797 8419 FAU - Ding, Xuanyan AU - Ding X AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - University of Chinese Academy of Sciences, Beijing, 100049 China. GRID: grid.410726.6. ISNI: 0000 0004 1797 8419 FAU - Li, Junyi AU - Li J AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - University of Chinese Academy of Sciences, Beijing, 100049 China. GRID: grid.410726.6. ISNI: 0000 0004 1797 8419 FAU - Chen, Ming AU - Chen M AD - School of Life Sciences, University of Science and Technology of China, Hefei, 230027 China. GRID: grid.59053.3a. ISNI: 0000000121679639 FAU - Zhu, Jinmin AU - Zhu J AD - GZMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, 511436 China. GRID: grid.410737.6. ISNI: 0000 0000 8653 1072 FAU - Mukama, Omar AU - Mukama O AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 FAU - Sun, Yirong AU - Sun Y AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 FAU - Li, Zhiyuan AU - Li Z AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - University of Chinese Academy of Sciences, Beijing, 100049 China. GRID: grid.410726.6. ISNI: 0000 0004 1797 8419 AD - School of Life Sciences, University of Science and Technology of China, Hefei, 230027 China. GRID: grid.59053.3a. ISNI: 0000000121679639 AD - GZMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, 511436 China. GRID: grid.410737.6. ISNI: 0000 0000 8653 1072 AD - GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, 410013 China. GRID: grid.216417.7. ISNI: 0000 0001 0379 7164 FAU - Huang, Rongqi AU - Huang R AD - CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 AD - GIBH-HKU Guangdong-Hong Kong Stem Cell and Regenerative Medicine Research Centre, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, 510530 China. GRID: grid.428926.3. ISNI: 0000 0004 1798 2725 LA - eng PT - Journal Article DEP - 20230308 PL - Germany TA - Bioresour Bioprocess JT - Bioresources and bioprocessing JID - 101665551 PMC - PMC9994782 OTO - NOTNLM OT - Biomedicine OT - Large-scale expansion culture OT - MSCs OT - PLGA@PLL scaffold COIS- Competing interestsThe authors declare no competing interests. EDAT- 2023/03/15 06:00 MHDA- 2023/03/15 06:01 PMCR- 2023/03/08 CRDT- 2023/03/14 02:09 PHST- 2022/11/10 00:00 [received] PHST- 2023/01/31 00:00 [accepted] PHST- 2023/03/14 02:09 [entrez] PHST- 2023/03/15 06:00 [pubmed] PHST- 2023/03/15 06:01 [medline] PHST- 2023/03/08 00:00 [pmc-release] AID - 635 [pii] AID - 10.1186/s40643-023-00635-6 [doi] PST - ppublish SO - Bioresour Bioprocess. 2023;10(1):18. doi: 10.1186/s40643-023-00635-6. Epub 2023 Mar 8.