PMID- 36918405
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230413
IS  - 2508-7576 (Electronic)
IS  - 2508-6235 (Print)
IS  - 2508-6235 (Linking)
VI  - 32
IP  - 1
DP  - 2023 Mar 30
TI  - Analyzing the Association of Visceral Adipose Tissue Growth Differentiation 
      Factor-15 and MicroRNA in Type 2 Diabetes Mellitus.
PG  - 64-76
LID - 10.7570/jomes22010 [doi]
AB  - BACKGROUND: Growth differentiation factor-15 (GDF-15) is involved in insulin 
      resistance and diabetes. In this study, we determine the associations of GDF-15 
      with miR-181b-5p, miR-330-3p, mothers against decapentaplegic homolog 7 (SMAD7), 
      and insulin resistance in visceral adipose tissue (VAT) and peripheral blood 
      mononuclear cells (PBMCs) in type 2 diabetes mellitus (T2DM) patients. METHODS: 
      Sixty patients, equally divided into those with T2DM and non-diabetic controls, 
      were recruited for gene expression analysis. Protein-protein interaction 
      (STRING), target prediction (miRNet), and functional enrichment were conducted 
      accordingly. RESULTS: Our study showed that VAT and PBMCs had similar expression 
      profiles, where GDF-15 and miR-181b-5p were upregulated, whereas SMAD7 and 
      miR-330-3p were downregulated. Serum GDF-15 could differentiate between T2DM and 
      non-diabetic patients (P<0.001). Target prediction revealed a microRNA 
      (miRNA)-messenger RNA regulatory network, transcription factors, and functional 
      enrichment for the miRNA that suggested involvement in T2DM pathogenesis. 
      CONCLUSION: VAT GDF-15 is associated with insulin resistance and is possibly 
      regulated by miR-181b-5p, miR-330-3p, and SMAD7 in T2DM.
FAU - Roy, Dipayan
AU  - Roy D
AD  - Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), 
      Jodhpur, India.
AD  - Indian Institute of Technology (ITT)-Madras, Chennai, India.
AD  - School of Humanities, Indira Gandhi National Open University (IGNOU), New Delhi, 
      India.
FAU - Purohit, Purvi
AU  - Purohit P
AUID- ORCID: 0000-0001-8559-2911
AD  - Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), 
      Jodhpur, India.
FAU - Khokhar, Manoj
AU  - Khokhar M
AD  - Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), 
      Jodhpur, India.
FAU - Modi, Anupama
AU  - Modi A
AD  - Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), 
      Jodhpur, India.
FAU - Shukla, Ravindra Kumar Gayaprasad
AU  - Shukla RKG
AD  - Department of Endocrinology and Metabolism, All India Institute of Medical 
      Sciences (AIIMS), Jodhpur, India.
FAU - Chaudhary, Ramkaran
AU  - Chaudhary R
AD  - Department of General Surgery, All India Institute of Medical Sciences (AIIMS), 
      Jodhpur, India.
FAU - Sankanagoudar, Shrimanjunath
AU  - Sankanagoudar S
AD  - Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), 
      Jodhpur, India.
FAU - Sharma, Praveen
AU  - Sharma P
AD  - Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), 
      Jodhpur, India.
LA  - eng
PT  - Journal Article
DEP - 20230315
PL  - Korea (South)
TA  - J Obes Metab Syndr
JT  - Journal of obesity & metabolic syndrome
JID - 101704724
PMC - PMC10088550
OTO - NOTNLM
OT  - Diabetes mellitus
OT  - Growth differentiation factor 15
OT  - Intra-abdominal fat
OT  - MicroRNAs
OT  - SMAD7
OT  - type 2
COIS- CONFLICTS OF INTEREST The authors declare no conflict of interest.
EDAT- 2023/03/16 06:00
MHDA- 2023/03/16 06:01
PMCR- 2023/03/15
CRDT- 2023/03/15 00:22
PHST- 2022/02/12 00:00 [received]
PHST- 2022/04/07 00:00 [revised]
PHST- 2023/03/07 00:00 [accepted]
PHST- 2023/03/16 06:01 [medline]
PHST- 2023/03/16 06:00 [pubmed]
PHST- 2023/03/15 00:22 [entrez]
PHST- 2023/03/15 00:00 [pmc-release]
AID - jomes22010 [pii]
AID - jomes-32-1-64 [pii]
AID - 10.7570/jomes22010 [doi]
PST - ppublish
SO  - J Obes Metab Syndr. 2023 Mar 30;32(1):64-76. doi: 10.7570/jomes22010. Epub 2023 
      Mar 15.