PMID- 36921536 OWN - NLM STAT- MEDLINE DCOM- 20230403 LR - 20230403 IS - 1950-6007 (Electronic) IS - 0753-3322 (Linking) VI - 161 DP - 2023 May TI - Apitoxin alleviates methyl mercury-induced peripheral neurotoxicity in male rats by regulating dorsal root ganglia neuronal degeneration and oxidative stress. PG - 114521 LID - S0753-3322(23)00309-8 [pii] LID - 10.1016/j.biopha.2023.114521 [doi] AB - Methylmercury (MeHg) toxicity is associated with extensive neuronal degeneration of dorsal root ganglia (DRG). This study aimed to assess the ameliorative effect of bee venom (BV) on methyl mercury chloride (MeHgCl)-induced peripheral neurotoxicity using DRGs in rats. Forty-eight adult male Sprague Dawley rats were allocated into four equal groups: G I: control (gavaged MilliQ water 1 ml/rat), G II: subcutaneously injected with BV (0.5 mg/kg b.wt), G III: gavaged MeHgCl (6.7 mg/kg b.wt), and G IV: received MeHgCl+BV. Dosing was done five times/week for 2 weeks. Ataxic behavior and visual impairments were significantly increased, whereas the movement behavior and motility gait were suppressed in the MeHgCl group. MeHgCl significantly decreased total antioxidant capacity (TAC) in DRG and significantly decreased the serum levels of glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD). Tumor necrosis factor-alpha (TNF-alpha) and interleukin 1beta (IL-1beta) levels were significantly elevated, whereas interleukin 10 (IL-10) levels were significantly decreased in the MeHgCl group compared with the control group. DRGs of the MeHgCl-exposed rats showed pyknotic shrunken neurons with perineural vacuolations, demyelination of nerve axons, and proliferation of the satellite cells. MeHgCl significantly induced a higher positive index ratio of Iba-1, SOX10, neurofilament, pan-neuron, and vimentin immunostaining in the DRG. BV administration significantly mitigated the MeHgCl-induced alterations in oxidative stress-related indices. BV modified the immunostaining of Iba-1, SOX10, neurofilament, pan-neuron, and vimentin-positive index ratio in the DRG of the MeHgCl group. Our findings acknowledged that BV could enhance in vivo neuroprotective effects against MeHgCl-induced DRGs damage in male rats. CI - Copyright (c) 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Abdelhamid, Moustafa S AU - Abdelhamid MS AD - Biochemistry division, Chemistry Department, Faculty of Science, Zagazig University, 44511, Egypt. FAU - El Bohi, Khlood M AU - El Bohi KM AD - Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt. FAU - Sherif, Mohamed H AU - Sherif MH AD - Biochemistry division, Chemistry Department, Faculty of Science, Zagazig University, 44511, Egypt. FAU - Abdelhamid, Manar S AU - Abdelhamid MS AD - Biochemistry division, Chemistry Department, Faculty of Science, Zagazig University, 44511, Egypt. FAU - Abdel-Daim, Mohamed M AU - Abdel-Daim MM AD - Department of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, P.O. Box 6231, Jeddah 21442, Saudi Arabia; Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. FAU - Elewa, Yaser H A AU - Elewa YHA AD - Department of Histology and Cytology, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt; Laboratory of Anatomy, Department of Basic Veterinary Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan. FAU - Metwally, Mohamed M M AU - Metwally MMM AD - Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt. FAU - Albadrani, Ghadeer M AU - Albadrani GM AD - Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, B.O. Box 84428, Riyadh 11671, Saudi Arabia. FAU - Najda, Agnieszka AU - Najda A AD - Department of Vegetable Crops and Medicinal Plants University of Life Sciences in Lublin, 50 A Doswiadczalna Street, 20-280 Lublin, Poland. Electronic address: agnieszka.najda@up.lublin.pl. FAU - El Abdel-Hamid, Shereen AU - El Abdel-Hamid S AD - Department of Behavior and Management of Animal, Poultry and Aquatics, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt. FAU - Abu-Zeid, Ehsan H AU - Abu-Zeid EH AD - Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, 44511, Egypt. Electronic address: ehelsharkawy@zu.edu.eg. LA - eng PT - Journal Article DEP - 20230313 PL - France TA - Biomed Pharmacother JT - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JID - 8213295 RN - 0 (Methylmercury Compounds) RN - 0 (Vimentin) RN - 0 (Bee Venoms) RN - GAN16C9B8O (Glutathione) RN - FXS1BY2PGL (Mercury) SB - IM MH - Rats MH - Animals MH - Male MH - *Methylmercury Compounds/toxicity MH - Rats, Sprague-Dawley MH - Vimentin MH - Ganglia, Spinal MH - *Bee Venoms/pharmacology MH - Oxidative Stress MH - Glutathione/pharmacology MH - *Mercury OTO - NOTNLM OT - Bee venom OT - Dorsal root ganglia OT - MeHgCl OT - Methyl mercury chloride OT - Neurodegeneration OT - Peripheral neurotoxicity COIS- Conflict of interest statement The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2023/03/16 06:00 MHDA- 2023/04/03 06:41 CRDT- 2023/03/15 19:15 PHST- 2023/01/06 00:00 [received] PHST- 2023/03/02 00:00 [revised] PHST- 2023/03/09 00:00 [accepted] PHST- 2023/04/03 06:41 [medline] PHST- 2023/03/16 06:00 [pubmed] PHST- 2023/03/15 19:15 [entrez] AID - S0753-3322(23)00309-8 [pii] AID - 10.1016/j.biopha.2023.114521 [doi] PST - ppublish SO - Biomed Pharmacother. 2023 May;161:114521. doi: 10.1016/j.biopha.2023.114521. Epub 2023 Mar 13.