PMID- 36923505
OWN - NLM
STAT- MEDLINE
DCOM- 20230320
LR  - 20230428
IS  - 2167-8359 (Electronic)
IS  - 2167-8359 (Linking)
VI  - 11
DP  - 2023
TI  - LTA4H extensively associates with mRNAs and lncRNAs indicative of its novel 
      regulatory targets.
PG  - e14875
LID - 10.7717/peerj.14875 [doi]
LID - e14875
AB  - The RNA-binding metabolic enzyme LTA4H is a novel target for cancer 
      chemoprevention and chemotherapy. Recent research shows that the increased 
      expression of LTA4H in laryngeal squamous cell carcinoma (LSCC) promotes tumor 
      proliferation, migration, and metastasis. However, its mechanism remains unclear. 
      To investigate the potential role of LTA4H in LSCC, we employed the improved RNA 
      immunoprecipitation and sequencing (iRIP-Seq) experiment to get the expression 
      profile of LTA4H binding RNA in HeLa model cells, a cancer model cell that is 
      frequently used in molecular mechanism research. We found that LTA4H extensively 
      binds with mRNAs/pre-mRNAs and lncRNAs. In the LTA4H binding peak, the frequency 
      of the AAGG motif reported to interact with TRA2beta4 was high in both replicates. 
      More notably, LTA4H-binding genes were significantly enriched in the mitotic cell 
      cycle, DNA repair, RNA splicing-related pathways, and RNA metabolism pathways, 
      which means that LTA4H has tumor-related alternative splicing regulatory 
      functions. QRT-PCR validation confirmed that LTA4H specifically binds to mRNAs of 
      carcinogenesis-associated genes, including LTBP3, ROR2, EGFR, HSP90B1, and 
      lncRNAs represented by NEAT1. These results suggest that LTA4H may combine with 
      genes associated with LSCC as an RNA-binding protein to perform a cancer 
      regulatory function. Our study further sheds light on the molecular mechanism of 
      LTA4H as a clinical therapy target for LSCC.
CI  - (c)2023 Ren et al.
FAU - Ren, Tianjiao
AU  - Ren T
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang, China.
FAU - Wang, Song
AU  - Wang S
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang, China.
FAU - Zhang, Bo
AU  - Zhang B
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang, China.
FAU - Zhou, Wei
AU  - Zhou W
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang, China.
FAU - Wang, Cansi
AU  - Wang C
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang, China.
FAU - Zhao, Xiaorui
AU  - Zhao X
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang, China.
FAU - Feng, Juan
AU  - Feng J
AD  - Department of Otorhinolaryngology, The First Affiliated Hospital of Xinjiang 
      Medical University, Urumqi, Xinjiang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230310
PL  - United States
TA  - PeerJ
JT  - PeerJ
JID - 101603425
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Humans
MH  - RNA, Messenger/genetics
MH  - *RNA, Long Noncoding/genetics
MH  - *Carcinoma, Squamous Cell/genetics
MH  - Gene Expression Profiling
MH  - *Laryngeal Neoplasms/genetics
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - *Head and Neck Neoplasms
PMC - PMC10010175
OTO - NOTNLM
OT  - LTA4H
OT  - Laryngeal tumorigenesis
OT  - RNA-binding protein
OT  - iRIP-seq
OT  - lncRNA
COIS- The authors declare there are no competing interests.
EDAT- 2023/03/17 06:00
MHDA- 2023/03/21 06:00
PMCR- 2023/03/10
CRDT- 2023/03/16 02:24
PHST- 2022/08/31 00:00 [received]
PHST- 2023/01/19 00:00 [accepted]
PHST- 2023/03/16 02:24 [entrez]
PHST- 2023/03/17 06:00 [pubmed]
PHST- 2023/03/21 06:00 [medline]
PHST- 2023/03/10 00:00 [pmc-release]
AID - 14875 [pii]
AID - 10.7717/peerj.14875 [doi]
PST - epublish
SO  - PeerJ. 2023 Mar 10;11:e14875. doi: 10.7717/peerj.14875. eCollection 2023.