PMID- 36923790
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230316
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 14
DP  - 2023
TI  - Formation of the synaptonemal complex in a gynogenetic allodiploid hybrid fish.
PG  - 998775
LID - 10.3389/fgene.2023.998775 [doi]
LID - 998775
AB  - Introduction: The correct pairing and separation of homologous chromosomes during 
      meiosis is crucial to ensure both genetic stability and genetic diversity within 
      species. In allodiploid organisms, synapsis often fails, leading to sterility. 
      However, a gynogenetic allodiploid hybrid clone line (GDH), derived by crossing 
      red crucian carp (Carassius auratus female symbol) and common carp (Cyprinus carpio male symbol), 
      stably produces diploid eggs. Because the GDH line carries 100 chromosomes with 
      50 chromosomes from the red crucian carp (RCC; female symbol, 2n = 2x = 100) and 50 
      chromosomes from the common carp (CC; C. carpio L., male symbol, 2n = 2x = 100), it is 
      interesting to study the mechanisms of homologous chromosome pairing during 
      meiosis in GDH individuals. Methods: By using fluorescence in situ hybridization 
      (FISH) with a probe specific to the red crucian carp to label homologous 
      chromosomes, we identified the synaptonemal complex via immunofluorescence assay 
      of synaptonemal complex protein 3 (SCP3). Results: FISH results indicated that, 
      during early ovarian development, the GDH oogonium had two sets of chromosomes 
      with only one set from Carassius auratus, leading to the failure formation of 
      normal bivalents and the subsequently blocking of meiosis. This inhibition lasted 
      at least 5 months. After this long period of inhibition, pairs of germ cells 
      fused, doubling the chromosomes such that the oocyte contained two sets of 
      chromosomes from each parent. After chromosome doubling at 10 months old, 
      homologous chromosomes and the synaptonemal complex were identified. Discussion: 
      Causally, meiosis proceeded normally and eventually formed diploid germ cells. 
      These results further clarify the mechanisms by which meiosis proceeds in 
      hybrids.
CI  - Copyright (c) 2023 Wang, Wang, Li, Zhang, Luo, Han, Xiang, Chai, Luo, Zhao and Liu.
FAU - Wang, Jing
AU  - Wang J
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Wang, Wen
AU  - Wang W
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Li, Jihong
AU  - Li J
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Zhang, Yirui
AU  - Zhang Y
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Luo, Kaikun
AU  - Luo K
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Han, Linmei
AU  - Han L
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Xiang, Caixia
AU  - Xiang C
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Chai, Mingli
AU  - Chai M
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Luo, Ziye
AU  - Luo Z
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Zhao, Rurong
AU  - Zhao R
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
FAU - Liu, Shaojun
AU  - Liu S
AD  - State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life 
      Sciences, Hunan Normal University, Changsha, China.
LA  - eng
PT  - Journal Article
DEP - 20230227
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC10009232
OTO - NOTNLM
OT  - gynogenesis allodiploid hybrids (GDH)
OT  - homologous chromosome
OT  - meiosis
OT  - synaptonemal complex
OT  - unreduced gametes
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/03/17 06:00
MHDA- 2023/03/17 06:01
PMCR- 2023/02/27
CRDT- 2023/03/16 02:29
PHST- 2022/07/20 00:00 [received]
PHST- 2023/02/17 00:00 [accepted]
PHST- 2023/03/16 02:29 [entrez]
PHST- 2023/03/17 06:00 [pubmed]
PHST- 2023/03/17 06:01 [medline]
PHST- 2023/02/27 00:00 [pmc-release]
AID - 998775 [pii]
AID - 10.3389/fgene.2023.998775 [doi]
PST - epublish
SO  - Front Genet. 2023 Feb 27;14:998775. doi: 10.3389/fgene.2023.998775. eCollection 
      2023.