PMID- 36927429
OWN - NLM
STAT- MEDLINE
DCOM- 20231201
LR  - 20231201
IS  - 2212-3946 (Electronic)
IS  - 1574-888X (Linking)
VI  - 19
IP  - 2
DP  - 2024
TI  - RADA-16-based Self-assembled Peptide Nanofiber Scaffolds Loaded with TGF-beta1 
      Enhance the Chondrogenic Differentiation Potential of BMSCs In vitro.
PG  - 257-266
LID - 10.2174/1574888X18666230316112847 [doi]
AB  - OBJECTIVE: At present, cartilage repair does not offer ideal efficacy. 
      Fortunately, recent studies have claimed that RADA-16 peptide is an attractive 
      therapeutic strategy for repairing cartilage defects. Therefore, this study tried 
      to explore the effect of RADA-16 loaded with transforming growth factor-beta 
      (TGF-beta) 1 on cartilage differentiation of bone marrow mesenchymal stem cells 
      (BMSCs). METHODS: First, the RADA-16 peptide was synthesized by solid phase 
      peptide, and a well-defined hydrogel was formed by supramolecular peptide 
      self-assembly. Then, TGF-beta1 (loading concentration of 10 ng/mL) was loaded into 
      RADA-16, with scanning electron microscopy to observe the morphology of the 
      TGF-beta1/RADA-16 hydrogel and detect its related properties. Next, BMSCs were 
      isolated from bone marrow samples and identified. TGF-beta1/RADA-16 was co-cultured 
      with L929, BMSCs, and C28/I2 cells, respectively, and the survival and 
      proliferation ability of the cells was determined by live/dead cell staining and 
      MTT assay. Chondrogenic differentiation and sGAG production of BMSCs were 
      determined by Alcian blue staining and Blyscan assay, the expression of 
      cartilage-associated genes by qRT-PCR, and the levels of inflammatory factors by 
      ELISA. As for mechanism investigation, the Smad and ERK/MAPK signaling pathways 
      were detected by western blot. RESULTS: RADA-16 hydrogel exhibited a 
      well-distributed and interconnected porous surface structure, with a loading rate 
      of 91.9% for TGF-beta1. The TGF-beta1/RADA-16 hydrogel had good release and degradation 
      properties, and had no negative effect on the survival and proliferation ability 
      of BMSCs, L929, and C28/I2 cells. Importantly, TGF-beta1/RADA-16 hydrogel 
      significantly accelerated chondrogenic differentiation and sGAG generation in 
      BMSCs, and decreased pro-inflammatory factor production. In addition, the 
      hydrogel also significantly activated the Smad and ERK/MAPK pathways of BMSCs. 
      CONCLUSION: RADA-16 loaded with TGF-beta1 has good biological properties and can 
      enhance the chondrogenic differentiation ability of BMSCs.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Yu, Peng
AU  - Yu P
AD  - Department of Joint Surgery, Center for Orthopaedic Surgery, The Third Affiliated 
      Hospital of Southern Medical University, The Third School of Clinical Medicine, 
      Southern Medical University, Guangzhou, 510515, China.
AD  - Department of Joint Surgery, The First Affiliated Hospital of Hainan Medical 
      University. Haikou, 570102, China.
FAU - Duan, Lian
AU  - Duan L
AD  - Department of Joint Surgery, The First Affiliated Hospital of Hainan Medical 
      University. Haikou, 570102, China.
FAU - Yan, Zhen
AU  - Yan Z
AD  - Department of Joint Surgery, The First Affiliated Hospital of Hainan Medical 
      University. Haikou, 570102, China.
FAU - Li, Jun
AU  - Li J
AD  - Department of Joint Surgery, The First Affiliated Hospital of Hainan Medical 
      University. Haikou, 570102, China.
FAU - Cai, Dao-Zhang
AU  - Cai DZ
AD  - Department of Joint Surgery, Center for Orthopaedic Surgery, The Third Affiliated 
      Hospital of Southern Medical University, The Third School of Clinical Medicine, 
      Southern Medical University, Guangzhou, 510515, China.
LA  - eng
GR  - 821RC703/Hainan Natural Science Foundation/
PT  - Journal Article
PL  - United Arab Emirates
TA  - Curr Stem Cell Res Ther
JT  - Current stem cell research & therapy
JID - 101272517
RN  - 0 (Hydrogels)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Humans
MH  - Bone Marrow Cells
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Chondrogenesis
MH  - Hydrogels/pharmacology/chemistry
MH  - *Mesenchymal Stem Cells
MH  - *Nanofibers
MH  - Transforming Growth Factor beta1/pharmacology
MH  - *Tissue Scaffolds
OTO - NOTNLM
OT  - MTT assay
OT  - RADA-16
OT  - bone marrow mesenchymal stem cells (BMSCs)
OT  - nano self-assembled polypeptide
OT  - proliferation.
OT  - transforming growth factor-beta (TGF-beta) 1
EDAT- 2023/03/18 06:00
MHDA- 2023/11/28 06:42
CRDT- 2023/03/17 08:21
PHST- 2022/11/22 00:00 [received]
PHST- 2023/02/03 00:00 [revised]
PHST- 2023/02/10 00:00 [accepted]
PHST- 2023/11/28 06:42 [medline]
PHST- 2023/03/18 06:00 [pubmed]
PHST- 2023/03/17 08:21 [entrez]
AID - CSCR-EPUB-130194 [pii]
AID - 10.2174/1574888X18666230316112847 [doi]
PST - ppublish
SO  - Curr Stem Cell Res Ther. 2024;19(2):257-266. doi: 
      10.2174/1574888X18666230316112847.