PMID- 36927981
OWN - NLM
STAT- MEDLINE
DCOM- 20230808
LR  - 20230823
IS  - 1881-7122 (Electronic)
IS  - 1341-1357 (Print)
IS  - 0007-5124 (Linking)
VI  - 72
IP  - 3
DP  - 2023 Aug 7
TI  - Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted 
      Treg/Th17 balance to Treg differentiation.
PG  - 367-378
LID - 10.1538/expanim.22-0171 [doi]
AB  - Autoimmune hepatitis (AIH) is a kind of autoimmune disease mediated by T cells, 
      and its incidence is gradually increasing in the world. Oroxylin A (OA) is one of 
      the major bioactive flavonoids that has been reported to inhibit inflammatory. 
      Here, an AIH model of mouse was induced by Concanavalin A (Con A). It found that 
      serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels 
      were decreased in mice with the treatment of OA. Hematoxylin-eosin staining 
      showed that the liver injury was attenuated by OA, and TUNEL staining indicated 
      that the cells apoptosis of liver was weakened in mice with OA treatment. ELISA 
      analysis of cytokines and chemokines suggested that OA reduced the expression of 
      IL-6, IL-17A, chemokine ligand 2 (CCL2), C-X-C motif chemokine ligand 1 (CXCL1) 
      and CXCL10, but promoted the expression of IL-10 and TGF-beta in mice. The mRNA 
      levels of Il-17a in liver and spleen tissues were also significantly decreased, 
      on the contrary, the mRNA levels of Il-10 in liver and spleen tissues were 
      increased. The proportion of Treg/Th17 detected by flow cytometry revealed that 
      OA promoted the differentiation of Treg and inhibited the differentiation of Th17 
      both in the liver and spleen. The results of this study demonstrated the 
      inhibitory effects of OA on AIH-induced liver injury and the inflammatory 
      response of AIH, and revealed that OA affected the balance of Treg/Th17 and 
      shifted the balance toward Treg differentiation. It provided new potential drugs 
      for the prevention of AIH.
FAU - Zhu, Jinxia
AU  - Zhu J
AD  - The First Clinical Medical College, Henan University of Chinese Medicine, 156 
      Jinshui East Road, Zhengzhou, Henan, 450046, P.R. China.
FAU - Chen, Hongxiu
AU  - Chen H
AD  - The First Clinical Medical College, Henan University of Chinese Medicine, 156 
      Jinshui East Road, Zhengzhou, Henan, 450046, P.R. China.
FAU - Cui, Jianjiao
AU  - Cui J
AD  - Spleen, Stomach and Hepatobiliary Department, The First Affiliated Hospital of 
      Henan University of Chinese Medicine, No. 19, Renmin Road, Zhengzhou, Henan, 
      450003, P.R. China.
FAU - Zhang, Xiaorui
AU  - Zhang X
AD  - Spleen, Stomach and Hepatobiliary Department, The First Affiliated Hospital of 
      Henan University of Chinese Medicine, No. 19, Renmin Road, Zhengzhou, Henan, 
      450003, P.R. China.
FAU - Liu, Guangwei
AU  - Liu G
AD  - Spleen, Stomach and Hepatobiliary Department, The First Affiliated Hospital of 
      Henan University of Chinese Medicine, No. 19, Renmin Road, Zhengzhou, Henan, 
      450003, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20230313
PL  - Japan
TA  - Exp Anim
JT  - Experimental animals
JID - 9604830
RN  - 130068-27-8 (Interleukin-10)
RN  - 0 (Interleukin-17)
RN  - 53K24Z586G (5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one)
RN  - 0 (Ligands)
RN  - 0 (Flavonoids)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Hepatitis, Autoimmune/drug therapy
MH  - Interleukin-10/metabolism
MH  - Interleukin-17/metabolism
MH  - T-Lymphocytes, Regulatory/metabolism
MH  - *Chemical and Drug Induced Liver Injury, Chronic
MH  - Ligands
MH  - Liver/metabolism
MH  - Flavonoids/pharmacology/metabolism/therapeutic use
MH  - Th17 Cells
MH  - Cell Differentiation
MH  - RNA, Messenger/metabolism
PMC - PMC10435359
OTO - NOTNLM
OT  - Th17
OT  - Treg
OT  - autoimmune hepatitis
OT  - oroxylin A
EDAT- 2023/03/18 06:00
MHDA- 2023/08/08 06:42
PMCR- 2023/01/01
CRDT- 2023/03/17 08:46
PHST- 2023/08/08 06:42 [medline]
PHST- 2023/03/18 06:00 [pubmed]
PHST- 2023/03/17 08:46 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 22-0171 [pii]
AID - 10.1538/expanim.22-0171 [doi]
PST - ppublish
SO  - Exp Anim. 2023 Aug 7;72(3):367-378. doi: 10.1538/expanim.22-0171. Epub 2023 Mar 
      13.