PMID- 36929887
OWN - NLM
STAT- MEDLINE
DCOM- 20230321
LR  - 20230701
IS  - 2544-4646 (Electronic)
IS  - 1733-1331 (Print)
IS  - 1733-1331 (Linking)
VI  - 72
IP  - 1
DP  - 2023 Mar 1
TI  - Characterization of a Plant Growth-Promoting Endohyphal Bacillus subtilis in 
      Fusarium acuminatum from Spiranthes sinensis.
PG  - 29-37
LID - 10.33073/pjm-2023-007 [doi]
AB  - Successful seed germination and seedling growth in orchids require an association 
      with mycorrhizal fungi. An endophytic Fusarium fungal strain YZU 172038 
      exhibiting plant growth-promoting (PGP) ability was isolated from the roots of 
      Spiranthes sinensis (Orchidaceae). The harboring endohyphal bacteria were 
      detected in the hypha by SYTO-9 fluorescent nucleic acid staining, fluorescence 
      in situ hybridization (FISH), and PCR amplification of the 16S rDNA gene's 
      region. Consequently, one endohyphal bacterium (EHB) - a strain YZSR384 was 
      isolated and identified as Bacillus subtilis based on morphology, phylogenetic 
      analysis, and genomic information. The results indicated that the strain YZSR384 
      could significantly promote the growth of rice roots and shoots similar to its 
      host fungus. Its indole acetic acid (IAA) production reached a maximum of 23.361 
      mug/ml on the sixth day after inoculation. The genome annotation revealed several 
      genes involved in PGP traits, including the clusters of genes encoding the IAA 
      (trpABCDEFS), the siderophores (entABCE), and the dissolving phosphate (pstABCS 
      and phoABDHPR). As an EHB, B. subtilis was first isolated from endophytic 
      Fusarium acuminatum from S. sinensis.
CI  - (c) 2023 LAN FANG et al., published by Sciendo.
FAU - Fang, Lan
AU  - Fang L
AD  - College of Agriculture, Yangtze University, Jingzhou, China.
FAU - Zheng, Xiao
AU  - Zheng X
AD  - College of Agriculture, Yangtze University, Jingzhou, China.
FAU - Sun, Zhengxiang
AU  - Sun Z
AD  - College of Agriculture, Yangtze University, Jingzhou, China.
FAU - Li, Yanyan
AU  - Li Y
AD  - Tobacco Research Institute of Hubei Province, Wuhan, China.
FAU - Deng, Jianxin
AU  - Deng J
AD  - College of Agriculture, Yangtze University, Jingzhou, China.
FAU - Zhou, Y I
AU  - Zhou YI
AD  - College of Agriculture, Yangtze University, Jingzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20230324
PL  - Poland
TA  - Pol J Microbiol
JT  - Polish journal of microbiology
JID - 101229003
RN  - Fusarium acuminatum
SB  - IM
MH  - Bacillus subtilis/genetics
MH  - *Fusarium/genetics
MH  - Phylogeny
MH  - In Situ Hybridization, Fluorescence
MH  - Fungi/genetics
MH  - *Orchidaceae/genetics
MH  - Plant Roots/microbiology
PMC - PMC10280306
OTO - NOTNLM
OT  - Bacillus subtilis
OT  - Fusarium acuminatum
OT  - endohyphal bacterium
OT  - plant growth-promoting ability
COIS- Conflict of interest: The authors do not report any financial or personal 
      connections with other persons or organizations, which might negatively affect 
      the contents of this publication and/or claim authorship rights to this 
      publication.
EDAT- 2023/03/18 06:00
MHDA- 2023/03/22 06:00
PMCR- 2023/03/24
CRDT- 2023/03/17 10:41
PHST- 2022/11/10 00:00 [received]
PHST- 2023/02/03 00:00 [accepted]
PHST- 2023/03/17 10:41 [entrez]
PHST- 2023/03/18 06:00 [pubmed]
PHST- 2023/03/22 06:00 [medline]
PHST- 2023/03/24 00:00 [pmc-release]
AID - pjm-2023-007 [pii]
AID - 10.33073/pjm-2023-007 [doi]
PST - epublish
SO  - Pol J Microbiol. 2023 Mar 24;72(1):29-37. doi: 10.33073/pjm-2023-007. Print 2023 
      Mar 1.