PMID- 36930562
OWN - NLM
STAT- MEDLINE
DCOM- 20230413
LR  - 20230421
IS  - 1530-6992 (Electronic)
IS  - 1530-6984 (Linking)
VI  - 23
IP  - 7
DP  - 2023 Apr 12
TI  - Pathogen Recognition-Driven Dendritic Cell-Specific Gene Silencing and Editing.
PG  - 2733-2742
LID - 10.1021/acs.nanolett.3c00015 [doi]
AB  - Dendritic cells (DCs) play an essential role in both the induction of the immune 
      response and the maintenance of immune tolerance, with any malfunction of DCs 
      potentially causing several diseases. While gene-based therapy for DC 
      manipulation is a promising approach, it remains challenging due to the lack of 
      efficient delivery systems for DC targeting. Herein, we describe a novel 
      bacterial nanomedicine (BNM) system for pathogen recognition-mediated 
      DCs-specific gene silencing and gene editing. BNMs contain components from 
      bacterial outer membranes and achieve efficient DC targeting through the 
      recognition of pathogen-associated molecular patterns by pattern recognition 
      receptors on DCs. The targeting efficiency of BNMs is reduced in DCs lacking 
      toll-like receptor 4, which is responsible for recognizing lipopolysaccharide, a 
      major component of the bacterial outer membrane. As a proof-of-concept 
      demonstration, we present gene-based therapy mediated by BNMs for enhancing 
      antigen cross-presentation in DCs, which generates a remarkable antitumor effect.
FAU - Li, Min
AU  - Li M
AD  - Department of Radiology, The First Affiliated Hospital of USTC, University of 
      Science and Technology of China, Hefei, Anhui 230001, China.
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
FAU - Zhou, Han
AU  - Zhou H
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
FAU - Wu, Namei
AU  - Wu N
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
FAU - Deng, Wuxian
AU  - Deng W
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
FAU - Dong, Wang
AU  - Dong W
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
FAU - Sun, Xun
AU  - Sun X
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
FAU - Liu, Hang
AU  - Liu H
AUID- ORCID: 0000-0002-4031-4851
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
FAU - Tian, Zhigang
AU  - Tian Z
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
FAU - Wang, Yucai
AU  - Wang Y
AUID- ORCID: 0000-0001-6046-2934
AD  - Department of Radiology, The First Affiliated Hospital of USTC, University of 
      Science and Technology of China, Hefei, Anhui 230001, China.
AD  - The Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic 
      Disease, School of Basic Medical Sciences, Division of Life Sciences and 
      Medicine, University of Science and Technology of China, Hefei 230027, China.
AD  - Institute of Health and Medicine, Hefei Comprehensive National Science Center, 
      Hefei, Anhui 230601, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230317
PL  - United States
TA  - Nano Lett
JT  - Nano letters
JID - 101088070
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - *Antigen Presentation
MH  - *Lipopolysaccharides
MH  - Dendritic Cells
MH  - Gene Silencing
OTO - NOTNLM
OT  - DC targeting
OT  - gene delivery
OT  - immunotherapy
OT  - nanoparticle
OT  - pathogen recognition
EDAT- 2023/03/18 06:00
MHDA- 2023/04/13 06:42
CRDT- 2023/03/17 12:53
PHST- 2023/04/13 06:42 [medline]
PHST- 2023/03/18 06:00 [pubmed]
PHST- 2023/03/17 12:53 [entrez]
AID - 10.1021/acs.nanolett.3c00015 [doi]
PST - ppublish
SO  - Nano Lett. 2023 Apr 12;23(7):2733-2742. doi: 10.1021/acs.nanolett.3c00015. Epub 
      2023 Mar 17.