PMID- 36933092
OWN - NLM
STAT- MEDLINE
DCOM- 20230328
LR  - 20230329
IS  - 1432-1211 (Electronic)
IS  - 0093-7711 (Linking)
VI  - 75
IP  - 2
DP  - 2023 Apr
TI  - Tumor necrosis factor-alpha induces proliferation and reduces apoptosis of colorectal 
      cancer cells through STAT3 activation.
PG  - 161-169
LID - 10.1007/s00251-023-01302-y [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is a potent pro-inflammatory factor that 
      plays an important role in establishing a complicated connection between 
      inflammation and cancer. TNF-alpha promotes tumor proliferation, migration, invasion, 
      and angiogenesis according to numerous studies. Studies have shown the 
      significant role of STAT3, a downstream transcription factor of another important 
      inflammatory cytokine, IL-6 in the development and progression of different 
      tumors especially colorectal cancer. In the present study, we investigated 
      whether TNF-alpha has a role in proliferation and apoptosis of colorectal cancer 
      cells through STAT3 activation. HCT116 cell line as human colorectal cancer cells 
      was used in this study. Major assays were MTT assay, reverse transcription-PCR 
      (RT-PCR), flow cytometric analysis, and ELISA. Results showed that TNF-alpha 
      significantly increased the phosphorylation of STAT3 and expression of all the 
      STAT3 target genes related to cell proliferation, survival, and metastasis 
      compared with control. Moreover, our data showed that the STAT3 phosphorylation 
      and expression of its target genes significantly were reduced in the presence of 
      TNF-alpha + STA-21 compared with TNF-alpha-treated group demonstrating that the increase 
      in genes expression partially was due to the TNF-alpha-induced STAT3 activation. On 
      the other hand, STAT3 phosphorylation and mRNA levels of its target genes were 
      partially decreased in the presence of TNF-alpha + IL-6R supporting the indirect 
      pathway of STAT3 activation by TNF-alpha through inducing IL-6 production in cancer 
      cells. Given the growing evidence for STAT3 as a key mediator of 
      inflammation-induced colon cancer, our findings support further investigation of 
      STAT3 inhibitors as potential cancer therapies.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Wei, Wei
AU  - Wei W
AD  - Department of Pathology, Xi'an No. 3 Hospital, the Affiliated Hospital of 
      Northwest University, Xi'an, Shaanxi, 710018, People's Republic of China.
FAU - Wang, Juanhong
AU  - Wang J
AD  - Department of Pathology, Xi'an No. 3 Hospital, the Affiliated Hospital of 
      Northwest University, Xi'an, Shaanxi, 710018, People's Republic of China.
FAU - Huang, Pu
AU  - Huang P
AD  - Department of Pathology, Xi'an Hospital of Traditional Chinese Medicine, Xi'an, 
      Shaanxi, 710021, People's Republic of China.
FAU - Gou, Siqi
AU  - Gou S
AD  - Department of Pathology, Xi'an No. 3 Hospital, the Affiliated Hospital of 
      Northwest University, Xi'an, Shaanxi, 710018, People's Republic of China.
FAU - Yu, Daihua
AU  - Yu D
AD  - Department of Critical Care Medicine, Xi'an No. 3 Hospital, the Affiliated 
      Hospital of Northwest University, Xi'an, Shaanxi, 710018, People's Republic of 
      China.
FAU - Zong, Lei
AU  - Zong L
AUID- ORCID: 0000-0002-3044-4476
AD  - Department of Critical Care Medicine, Xi'an No. 3 Hospital, the Affiliated 
      Hospital of Northwest University, Xi'an, Shaanxi, 710018, People's Republic of 
      China. zonglei7910@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20230318
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Interleukin-6)
RN  - 0 (STAT3 protein, human)
RN  - 0 (STAT3 Transcription Factor)
SB  - IM
MH  - Humans
MH  - *Tumor Necrosis Factor-alpha/genetics
MH  - Interleukin-6/genetics
MH  - Cell Proliferation
MH  - Apoptosis
MH  - *Colorectal Neoplasms/genetics/metabolism/pathology
MH  - Inflammation
MH  - STAT3 Transcription Factor/genetics/metabolism
OTO - NOTNLM
OT  - Colorectal cancer
OT  - IL-6
OT  - STAT3
OT  - TNF- alpha
EDAT- 2023/03/19 06:00
MHDA- 2023/03/28 17:16
CRDT- 2023/03/18 12:15
PHST- 2023/01/14 00:00 [received]
PHST- 2023/02/14 00:00 [accepted]
PHST- 2023/03/28 17:16 [medline]
PHST- 2023/03/19 06:00 [pubmed]
PHST- 2023/03/18 12:15 [entrez]
AID - 10.1007/s00251-023-01302-y [pii]
AID - 10.1007/s00251-023-01302-y [doi]
PST - ppublish
SO  - Immunogenetics. 2023 Apr;75(2):161-169. doi: 10.1007/s00251-023-01302-y. Epub 
      2023 Mar 18.