PMID- 36934264
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230601
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 23
IP  - 1
DP  - 2023 Mar 18
TI  - Exosomal MFI2-AS1 sponge miR-107 promotes non-small cell lung cancer progression 
      through NFAT5.
PG  - 51
LID - 10.1186/s12935-023-02886-x [doi]
LID - 51
AB  - BACKGROUND: Non-small cell lung cancer is a heterogeneous disease driven by 
      extensive molecular alterations. Exosomes are small vesicles with diameters 
      ranging from 30 to 150 nm released by various cell types and are important 
      mediators of information transmission in tumor cells. Exosomes contain proteins, 
      lipids, and various types of nucleic acids, including miRNAs and even DNA and 
      RNA. MFI2 Antisense RNA 1 (MFI2-AS1) is a long noncoding RNA known to promote 
      cell proliferation, metastasis and invasion in a variety of malignancies. 
      METHODS: The relative expression of MFI2-AS1 in NSCLC tissues was examined using 
      RNA fluorescence in situ hybridization (FISH) staining. Transwell migration and 
      wound healing assays were used to analyze cell migration and invasion abilities. 
      Tube formation is used to assess angiogenic capacity. CCK8 was used to assess 
      cell proliferation ability. RNA immunoprecipitation (RIP) experiments confirmed 
      that MFI2-AS1 acts as a competing endogenous RNA (ceRNA) for miR-107. 
      Dual-luciferase reporter assays were used to identify potential binding between 
      MFI2-miRNA and target mRNA. In vivo experiments were performed by injecting 
      exosomes into subcutaneous tumors to establish animal models. RESULT: Exosomal 
      MFI2-AS1 increases NFAT5 expression by sponging miR-107, which in turn activates 
      the PI3K/AKT pathway. We found that the MFI2-AS1/miR-107/NFAT5 axis plays an 
      important role in exosome-mediated NSCLC progression, is involved in 
      pre-metastatic niche formation, and can be used as a blood-based biomarker for 
      NSCLC metastasis. CONCLUSION: We demonstrate that MFI2-AS1 is upregulated in 
      exosomes secreted by metastatic NSCLC cells and can be transferred to HUVECs, 
      promoting angiogenesis and migration.
CI  - (c) 2023. The Author(s).
FAU - Xu, Jingwei
AU  - Xu J
AD  - Department of Cardiothoracic Surgery, The Affiliated Suzhou Hospital of Nanjing 
      Medical University, Suzhou Municipal Hospital, Suzhou, 215002, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Pulmonary and Critical Care Medicine, The Affiliated Suzhou 
      Hospital of Nanjing Medical University, Suzhou Municipal Hospital, 26 Daoqian 
      Street, Gusu District, Suzhou, 215002, China.
FAU - Shi, Baoyu
AU  - Shi B
AD  - Department of Pulmonary and Critical Care Medicine, The Affiliated Suzhou 
      Hospital of Nanjing Medical University, Suzhou Municipal Hospital, 26 Daoqian 
      Street, Gusu District, Suzhou, 215002, China.
FAU - Li, Ning
AU  - Li N
AD  - Department of Pulmonary and Critical Care Medicine, The Affiliated Suzhou 
      Hospital of Nanjing Medical University, Suzhou Municipal Hospital, 26 Daoqian 
      Street, Gusu District, Suzhou, 215002, China.
FAU - Xu, Guopeng
AU  - Xu G
AD  - Department of Pulmonary and Critical Care Medicine, The Affiliated Suzhou 
      Hospital of Nanjing Medical University, Suzhou Municipal Hospital, 26 Daoqian 
      Street, Gusu District, Suzhou, 215002, China.
FAU - Yan, Xiaopei
AU  - Yan X
AD  - Department of Pulmonary and Critical Care Medicine, The Affiliated Suzhou 
      Hospital of Nanjing Medical University, Suzhou Municipal Hospital, 26 Daoqian 
      Street, Gusu District, Suzhou, 215002, China. 497976246@qq.com.
FAU - Xu, Li
AU  - Xu L
AD  - Department of Pulmonary and Critical Care Medicine, The Affiliated Suzhou 
      Hospital of Nanjing Medical University, Suzhou Municipal Hospital, 26 Daoqian 
      Street, Gusu District, Suzhou, 215002, China. wuxiaohan200901@sina.com.
LA  - eng
PT  - Journal Article
DEP - 20230318
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC10024841
OTO - NOTNLM
OT  - MFI2-AS1
OT  - NFAT5
OT  - Non-small cell lung cancer
OT  - Pre-metastatic niche formation
OT  - miR-107
COIS- The authors declare that there are no conflict of interests.
EDAT- 2023/03/20 06:00
MHDA- 2023/03/20 06:01
PMCR- 2023/03/18
CRDT- 2023/03/19 00:25
PHST- 2022/04/14 00:00 [received]
PHST- 2023/03/03 00:00 [accepted]
PHST- 2023/03/19 00:25 [entrez]
PHST- 2023/03/20 06:00 [pubmed]
PHST- 2023/03/20 06:01 [medline]
PHST- 2023/03/18 00:00 [pmc-release]
AID - 10.1186/s12935-023-02886-x [pii]
AID - 2886 [pii]
AID - 10.1186/s12935-023-02886-x [doi]
PST - epublish
SO  - Cancer Cell Int. 2023 Mar 18;23(1):51. doi: 10.1186/s12935-023-02886-x.