PMID- 36934562
OWN - NLM
STAT- MEDLINE
DCOM- 20230425
LR  - 20230425
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 118
DP  - 2023 May
TI  - Oroxylin A relieves intrauterine adhesion in mice through inhibiting macrophage 
      pyroptosis via SIRT3-SOD2-ROS pathway.
PG  - 110023
LID - S1567-5769(23)00344-2 [pii]
LID - 10.1016/j.intimp.2023.110023 [doi]
AB  - Intrauterine adhesion (IUA) is manifested by endometrial fibrosis and 
      inflammation, which seriously affects female reproductive health. Macrophages are 
      mainly inflammatory cells and have been reported to participate in the fibrosis 
      of IUA. Oroxylin A (OA), a kind of flavonoid compounds, was showed to possess the 
      inhibitory effects on inflammation and fibrosis. However, the role of OA in IUA 
      remains unclear. In the present study, we found that OA effectively alleviated 
      the level of inflammation and uterine fibrosis in IUA mice. OA also decreased the 
      macrophage pyroptosis which increased in uteri of IUA mice. Pyroptosis is a 
      programmed cell death accompanied by an inflammatory response. Moreover, OA 
      repressed the mediators of pyroptosis including the expression of NOD-like 
      receptor family pyrin domain containing 3 (NLRP3), caspase-1 and Gasdermin D 
      (GSDMD) and the release of IL-1beta, IL-18 and cleaved-caspase-1 in J774A.1 cells 
      induced by LPS/ATP in vitro. Mechanistically, the alleviation of OA on uterine 
      fibrosis is achieved by inhibiting macrophage pyroptosis via SIRT3-SOD2-ROS 
      pathway. Our data indicate that OA may serve as an effective agent for the 
      treatment of the endometrial fibrosis with IUA.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Yang, Jingjing
AU  - Yang J
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing 210093, China.
FAU - Li, Jingman
AU  - Li J
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing 210093, China.
FAU - Wang, Jiali
AU  - Wang J
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing 210093, China.
FAU - Wu, Jinjin
AU  - Wu J
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing 210093, China.
FAU - Yin, Lijie
AU  - Yin L
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing 210093, China.
FAU - Dou, Huan
AU  - Dou H
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory 
      of Molecular Medicine, Medical School, Nanjing University, Nanjing 210093, China. 
      Electronic address: douhuan@nju.edu.cn.
FAU - Hou, Yayi
AU  - Hou Y
AD  - The State Key Laboratory of Pharmaceutical Biotechnology, Division of Immunology, 
      Medical School, Nanjing University, Nanjing 210093, China; Jiangsu Key Laboratory 
      of Molecular Medicine, Medical School, Nanjing University, Nanjing 210093, China. 
      Electronic address: yayihou@nju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20230317
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Reactive Oxygen Species)
RN  - 53K24Z586G (5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one)
RN  - EC 3.5.1.- (Sirtuin 3)
RN  - 0 (Flavonoids)
RN  - EC 3.4.22.36 (Caspase 1)
RN  - 0 (Sirt3 protein, mouse)
SB  - IM
MH  - Mice
MH  - Female
MH  - Animals
MH  - *Inflammasomes/metabolism
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - *Sirtuin 3/metabolism
MH  - Pyroptosis
MH  - Macrophages/metabolism
MH  - Flavonoids/pharmacology/therapeutic use
MH  - Caspase 1/metabolism
MH  - Inflammation/metabolism
MH  - Fibrosis
OTO - NOTNLM
OT  - Fibrosis
OT  - Intrauterine adhesion
OT  - Macrophage pyroptosis
OT  - Oroxylin A
OT  - SIRT3-SOD2-ROS pathway
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/03/20 06:00
MHDA- 2023/04/25 06:42
CRDT- 2023/03/19 19:04
PHST- 2022/11/01 00:00 [received]
PHST- 2023/03/01 00:00 [revised]
PHST- 2023/03/08 00:00 [accepted]
PHST- 2023/04/25 06:42 [medline]
PHST- 2023/03/20 06:00 [pubmed]
PHST- 2023/03/19 19:04 [entrez]
AID - S1567-5769(23)00344-2 [pii]
AID - 10.1016/j.intimp.2023.110023 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2023 May;118:110023. doi: 10.1016/j.intimp.2023.110023. Epub 
      2023 Mar 17.