PMID- 36934957 OWN - NLM STAT- MEDLINE DCOM- 20230607 LR - 20230611 IS - 1090-2392 (Electronic) IS - 0011-2240 (Linking) VI - 111 DP - 2023 Jun TI - Effects of sericin on oxidative stress and PI3K/AKT/mTOR signal pathway in cryopreserved mice ovarian tissue. PG - 16-25 LID - S0011-2240(23)00025-1 [pii] LID - 10.1016/j.cryobiol.2023.03.003 [doi] AB - Ovarian tissue cryopreservation is an effective fertility protective strategy for preadolescent female cancer patients, whose tumor treatment cannot be delayed. In the present study, the effects of sericin, as an antioxidant, on mice ovarian tissue freezing and thawing were investigated. Mice ovarian tissues were cryopreserved and thawed in medium containing 0.5% or 1%sericin (w/v), and 0.1 mM melatonin. Then, the follicular morphology was observed. The levels of antioxidant enzymes were determined, including glutathione (GSH), glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), total antioxidant capacity (T-AOC) and catalase (CAT). Moreover, the levels of nitric oxide (NO), malondialdehyde (MDA) and lactate dehydrogenase (LDH) were also tested. Besides, apoptosis-related proteins Bcl-2 and Bax were determined. Our results showed that 1% sericin maintained follicular morphology, inhibited apoptosis, decreased MDA and NO levels, and boosted endogenous antioxidant enzyme levels, while had no significant effect on LDH levels. Furthermore, these effects may be related with the activation of the phosphatidylinositol-3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of Rapamycin (mTOR) signaling pathway, as demonstrated by increased PI3K, p-AKT and mTOR levels. These findings demonstrate that 1% sericin may reduce oxidative stress and protect ovarian tissues during freezing and thawing via PI3K/AKT/mTOR signaling pathway. CI - Copyright (c) 2023 Elsevier Inc. All rights reserved. FAU - Shu, Wei Han AU - Shu WH AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Yang, Song He AU - Yang SH AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Wei, Meng AU - Wei M AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Liu, Xiao Chao AU - Liu XC AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Chen, Zi Xuan AU - Chen ZX AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Wei, Chen Yang AU - Wei CY AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Zhang, Xin AU - Zhang X AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Si, Li Na AU - Si LN AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Chen, Zhi Hong AU - Chen ZH AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Qiao, Yue Bing AU - Qiao YB AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. FAU - Li, Dong Mei AU - Li DM AD - HLA Laboratory, Beijing Red Cross Blood Center, Beijing, 100088, China. Electronic address: 30221188@qq.com. FAU - Sun, Tie Cheng AU - Sun TC AD - HLA Laboratory, Beijing Red Cross Blood Center, Beijing, 100088, China; Reproductive Medical Center, Department of Obstetrics and Gynecology, Peking University International Hospital, Beijing, 102206, China. Electronic address: zufengbenpao@163.com. FAU - Cheng, Lu Yang AU - Cheng LY AD - Faculty of Graduate Studies, Chengde Medical University, Hebei, 067000, China; Department of Immunology, Basic Medical College, Chengde Medical University, Hebei, 067000, China. Electronic address: yxyysh@126.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230317 PL - Netherlands TA - Cryobiology JT - Cryobiology JID - 0006252 RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase) RN - EC 2.7.1.- (Phosphatidylinositol 3-Kinases) RN - 0 (Sericins) RN - 0 (Antioxidants) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - GAN16C9B8O (Glutathione) SB - IM MH - Female MH - Mice MH - Animals MH - *Proto-Oncogene Proteins c-akt/metabolism MH - Phosphatidylinositol 3-Kinase/metabolism/pharmacology MH - Phosphatidylinositol 3-Kinases/metabolism/pharmacology MH - *Sericins/pharmacology MH - Antioxidants/pharmacology/metabolism MH - Cryopreservation/methods MH - Signal Transduction MH - TOR Serine-Threonine Kinases/metabolism/pharmacology MH - Oxidative Stress MH - Glutathione/pharmacology MH - Apoptosis MH - Mammals/metabolism OTO - NOTNLM OT - Antioxidant OT - Ovarian tissue cryopreservation OT - PI3K/AKT/mTOR signaling pathway OT - Sericin COIS- Declaration of competing interest The authors declare that they have no conflict of interest. EDAT- 2023/03/20 06:00 MHDA- 2023/06/07 06:42 CRDT- 2023/03/19 20:30 PHST- 2023/01/04 00:00 [received] PHST- 2023/03/02 00:00 [revised] PHST- 2023/03/10 00:00 [accepted] PHST- 2023/06/07 06:42 [medline] PHST- 2023/03/20 06:00 [pubmed] PHST- 2023/03/19 20:30 [entrez] AID - S0011-2240(23)00025-1 [pii] AID - 10.1016/j.cryobiol.2023.03.003 [doi] PST - ppublish SO - Cryobiology. 2023 Jun;111:16-25. doi: 10.1016/j.cryobiol.2023.03.003. Epub 2023 Mar 17.