PMID- 3693541
OWN - NLM
STAT- MEDLINE
DCOM- 19880212
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 25
IP  - 11
DP  - 1987 Nov
TI  - Indirect enzyme-linked immunosorbent assay for measurement of human 
      immunoglobulins E and G to purified cow's milk proteins: application in diagnosis 
      of cow's milk allergy.
PG  - 2114-9
AB  - An indirect double-antibody enzyme-linked immunosorbent assay (ELISA) was 
      developed for the measurement of human immunoglobulin E (IgE) and IgG to the 
      cow's milk proteins (CMP) alpha-casein, alpha-lactalbumin, and 
      beta-lactoglobulin. Human serum albumin was used as the negative-antigen control. 
      Rabbit anti-human IgE or IgG served as the primary antibody, and horseradish 
      peroxidase-conjugated swine anti-rabbit immunoglobulin served as the secondary 
      antibody. Positive control sera were obtained from patients with well-documented 
      histories of cow's milk allergy, while negative control sera were obtained from 
      cord bloods of healthy full-term infants and from normal adult volunteers without 
      known milk allergy. Test sera were obtained from 41 children (ages, 3 months to 
      13 years; average age, 2.6 years) with suspected cow's milk allergy and clinical 
      manifestations that included wheezing, rhinitis, atopic dermatitis, urticaria, or 
      gastrointestinal disturbances. The patients were simultaneously evaluated by 
      prick skin testing with scratch test antigen to whole CMP. Although only 13 (32%) 
      of the 41 patients were positive by the prick skin test, 25 (61%) were positive 
      by the IgE ELISA. Of the 25 IgE ELISA-positive patients, 20 were also positive by 
      the IgG ELISA. There was concordance of positive results between skin testing and 
      the IgE ELISA in only 9 patients (22%), and there was concordance of negative 
      results in 12 patients (29%). Discordant results were observed in 20 patients 
      (49%). These results indicate that the ELISA is more sensitive than prick skin 
      testing in the identification of individuals with elevated levels of IgE to CMP.
FAU - Campbell, D E
AU  - Campbell DE
AD  - Clinical Immunology Laboratory, Children's Hospital of Philadelphia, Pennsylvania 
      19104.
FAU - Ngamphaiboon, J
AU  - Ngamphaiboon J
FAU - Clark, M M
AU  - Clark MM
FAU - Harris, M C
AU  - Harris MC
FAU - Kolski, G B
AU  - Kolski GB
FAU - Douglas, S D
AU  - Douglas SD
LA  - eng
GR  - HL-27068/HL/NHLBI NIH HHS/United States
GR  - NS17752/NS/NINDS NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Caseins)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Lactoglobulins)
RN  - 0 (Milk Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9013-90-5 (Lactalbumin)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Caseins/immunology
MH  - Child
MH  - Child, Preschool
MH  - *Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Fetal Blood/immunology
MH  - Food Hypersensitivity/*diagnosis
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Immunoglobulin G/*analysis
MH  - Infant
MH  - Infant, Newborn
MH  - Lactalbumin/immunology
MH  - Lactoglobulins/immunology
MH  - Male
MH  - Milk/*adverse effects
MH  - Milk Proteins/*immunology
MH  - Skin Tests
PMC - PMC269422
EDAT- 1987/11/01 00:00
MHDA- 1987/11/01 00:01
PMCR- 1987/11/01
CRDT- 1987/11/01 00:00
PHST- 1987/11/01 00:00 [pubmed]
PHST- 1987/11/01 00:01 [medline]
PHST- 1987/11/01 00:00 [entrez]
PHST- 1987/11/01 00:00 [pmc-release]
AID - 10.1128/jcm.25.11.2114-2119.1987 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1987 Nov;25(11):2114-9. doi: 10.1128/jcm.25.11.2114-2119.1987.