PMID- 36940958
OWN - NLM
STAT- MEDLINE
DCOM- 20230322
LR  - 20231216
IS  - 1658-3175 (Electronic)
IS  - 0379-5284 (Print)
IS  - 0379-5284 (Linking)
VI  - 44
IP  - 3
DP  - 2023 Mar
TI  - Phenotypical evaluation of lymphocytes and monocytes in patients with type 2 
      diabetes mellitus in Saudi Arabia.
PG  - 296-305
LID - 10.15537/smj.2023.44.3.20220873 [doi]
AB  - OBJECTIVES: To evaluate the levels of total lymphocytes, B-lymphocytes (CD19+), 
      T-lymphocytes (CD3+), natural killer (NK) cells (CD3-/CD56+), and monocyte 
      subsets in type 2 diabetes mellitus (T2DM) patients in Saudi Arabia. In addition, 
      this study aimed to evaluate whether B- and T-lymphocyte subsets are frequently 
      altered in patients with T2DM. METHODS: A case-control study included 95 
      participants recruited in the study: 62 patients with T2DM and 33 healthy 
      individuals. All the patients were admitted to the Diabetic Centre in Taif, Saudi 
      Arabia. Blood samples were collected between April and August 2022. The 
      hemoglobin A1c (HbA1c) level was evaluated in all patients. Flow cytometry was 
      used to measure the expression of B-lymphocyte, T-lymphocyte, NK cells, and 
      monocyte markers. The unpaired t-test was carried out to evaluate the differences 
      in these markers between T2DM patients and healthy individuals. RESULTS: Patients 
      with T2DM were associated with a lower percentage of total lymphocytes, higher 
      percentage of B-lymphocytes, naive, and memory B subsets. In addition, patients 
      with T2DM showed lower percentage of total T-lymphocytes (CD3+) and CD4 T-cells, 
      but higher CD8 T-cell expression. Also, the NK-cell level was reduced in patients 
      with T2DM, and the levels of monocyte subsets were altered. CONCLUSION: These 
      data suggested that levels of lymphocytes and monocytes are impaired in T2DM 
      patients, and this might be associated with the higher risk of infections 
      observed in these patients.
CI  - Copyright: (c) Saudi Medical Journal.
FAU - Allahyani, Mamdouh
AU  - Allahyani M
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Alshalawi, Amani M
AU  - Alshalawi AM
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Alshalawii, Maram R
AU  - Alshalawii MR
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Alqorashi, Shahad A
AU  - Alqorashi SA
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Aljuaid, Abdulelah
AU  - Aljuaid A
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Almehmadi, Mazen M
AU  - Almehmadi MM
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Bokhary, Mohammed A
AU  - Bokhary MA
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Albrgey, Alhanouf S
AU  - Albrgey AS
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Alghamdi, Ahmad A
AU  - Alghamdi AA
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Aldairi, Abdullah F
AU  - Aldairi AF
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
FAU - Alhazmi, Ayman S
AU  - Alhazmi AS
AD  - From the Department of Clinical Laboratory Sciences (Allahyani, Alshalawi, 
      Alshalawii, Alqorashi, Aljuaid, Almehmadi, Alghamdi, Alhazmi), College of Applied 
      Medical Sciences, Taif University, from the Department of Clinical Chemistry 
      (Bokhar); from the Department of Endocrinology and Diabetic Centre (Albrgey), 
      King Abdulaziz specialist hospital, Ministry of Health, Taif, and from the 
      Department of Laboratory Medicine (Aldairi), Faculty of Applied Medical Sciences, 
      Umm Al-Qura University, Makkah, Kingdom of Saudi Arabia.
LA  - eng
PT  - Journal Article
PL  - Saudi Arabia
TA  - Saudi Med J
JT  - Saudi medical journal
JID - 7909441
SB  - IM
MH  - Humans
MH  - *Monocytes
MH  - Case-Control Studies
MH  - *Diabetes Mellitus, Type 2/metabolism
MH  - Saudi Arabia
MH  - B-Lymphocytes/metabolism
MH  - Flow Cytometry
PMC - PMC10043885
OTO - NOTNLM
OT  - T2DM
OT  - lymphocytes
OT  - monocytes
EDAT- 2023/03/21 06:00
MHDA- 2023/03/23 06:00
PMCR- 2023/03/01
CRDT- 2023/03/20 20:33
PHST- 2022/12/17 00:00 [received]
PHST- 2023/02/15 00:00 [accepted]
PHST- 2023/03/20 20:33 [entrez]
PHST- 2023/03/21 06:00 [pubmed]
PHST- 2023/03/23 06:00 [medline]
PHST- 2023/03/01 00:00 [pmc-release]
AID - 44/3/296 [pii]
AID - SaudiMedJ-44-3-296 [pii]
AID - 10.15537/smj.2023.44.3.20220873 [doi]
PST - ppublish
SO  - Saudi Med J. 2023 Mar;44(3):296-305. doi: 10.15537/smj.2023.44.3.20220873.