PMID- 36943006
OWN - NLM
STAT- MEDLINE
DCOM- 20230605
LR  - 20230605
IS  - 1475-097X (Electronic)
IS  - 1475-0961 (Linking)
VI  - 43
IP  - 4
DP  - 2023 Jul
TI  - Increasing circulating levels of Tenascin C in response to the Wingate anaerobic 
      test.
PG  - 271-277
LID - 10.1111/cpf.12816 [doi]
AB  - AIM: Tenascin C (TNC) is a large extracellular matrix glycoprotein. It is 
      involved in development and upregulated both during tissue repair and in several 
      pathological conditions, including cardiovascular disease. Extracellular matrix 
      proteins play a role in promoting exercise responses, leading to adaptation, 
      regeneration, and repair. The main goal of this study was to investigate whether 
      a short anaerobic effort leads to increased levels of TNC in serum. METHODS: 
      Thirty-nine healthy men performed a Wingate test followed by a muscle biopsy. 
      Myoblasts were isolated from the muscle biopsies and differentiated to myotubes 
      ex vivo. TNC RNA was quantified in the biopsies, myotubes and myoblasts using RNA 
      sequencing. Blood samples were drawn before and 5 min after the Wingate test. 
      Serum TNC levels were measured using enzyme-linked immunosorbent assay. RESULTS: 
      After the Wingate test, serum TNC increased on average by 23% [15-33], median 
      [interquartile range]; P(Wilcoxon)  < 0.0001. This increase is correlated with 
      peak power output and power drop, but not with VO(2max) . TNC RNA expression is 
      higher in myoblasts and myotubes compared to skeletal muscle tissue. CONCLUSION: 
      TNC is secreted systemically as a response to the Wingate anaerobic test in 
      healthy males. The response was positively correlated with peak power and power 
      drop, but not with VO(2max) which implicates a relation to mechanical strain 
      and/or blood flow. With higher expression in undifferentiated myoblast cells than 
      muscle tissue, it is likely that TNC plays a role in muscle tissue remodelling in 
      humans. Our findings open for research on how TNC contributes to exercise 
      adaptation.
CI  - (c) 2023 The Authors. Clinical Physiology and Functional Imaging published by John 
      Wiley & Sons Ltd on behalf of Scandinavian Society of Clinical Physiology and 
      Nuclear Medicine.
FAU - Ekstrom, Ola
AU  - Ekstrom O
AUID- ORCID: 0000-0002-4857-0892
AD  - Department of Clinical Sciences, Lund University Diabetes Centre, Lund 
      University, Malmo, Sweden.
FAU - Strom, Kristoffer
AU  - Strom K
AD  - Department of Clinical Sciences, Lund University Diabetes Centre, Lund 
      University, Malmo, Sweden.
FAU - Mir, Bilal Ahmad
AU  - Mir BA
AD  - Department of Clinical Sciences, Lund University Diabetes Centre, Lund 
      University, Malmo, Sweden.
FAU - Laurila, Esa
AU  - Laurila E
AD  - Department of Clinical Sciences, Lund University Diabetes Centre, Lund 
      University, Malmo, Sweden.
FAU - Wessman, Ylva
AU  - Wessman Y
AD  - Department of Clinical Sciences, Lund University Diabetes Centre, Lund 
      University, Malmo, Sweden.
FAU - Lehtovirta, Mikko
AU  - Lehtovirta M
AD  - Department of Clinical Sciences, Lund University Diabetes Centre, Lund 
      University, Malmo, Sweden.
AD  - Institute for Molecular Medicine Finland (FIMM), Helsinki University, Helsinki, 
      Finland.
FAU - Eriksson, Karl-Fredrik
AU  - Eriksson KF
AD  - Department of Clinical Sciences, Lund University Diabetes Centre, Lund 
      University, Malmo, Sweden.
FAU - Hansson, Ola
AU  - Hansson O
AD  - Department of Clinical Sciences, Lund University Diabetes Centre, Lund 
      University, Malmo, Sweden.
AD  - Institute for Molecular Medicine Finland (FIMM), Helsinki University, Helsinki, 
      Finland.
LA  - eng
GR  - Crafoordska Stiftelsen/
GR  - Diabetesforbundet/
GR  - IRC15-0067/Stiftelsen for Strategisk Forskning/
GR  - Novo Nordisk Fonden/
GR  - Hjelt Foundation/
GR  - Knut och Alice Wallenbergs Stiftelse/
GR  - Insamlingsstiftelsen Diabetes Wellness Network Sverige/
GR  - 2009-1039/Vetenskapsradet/
GR  - 2018-02635/Vetenskapsradet/
GR  - Direktor Albert Pahlssons Stiftelse/
PT  - Journal Article
DEP - 20230402
PL  - England
TA  - Clin Physiol Funct Imaging
JT  - Clinical physiology and functional imaging
JID - 101137604
RN  - 0 (Tenascin)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Male
MH  - Humans
MH  - *Tenascin/genetics/metabolism
MH  - Anaerobiosis
MH  - *Extracellular Matrix Proteins/metabolism
MH  - Extracellular Matrix/metabolism
MH  - RNA/metabolism
OTO - NOTNLM
OT  - TNC
OT  - Tenascin
OT  - Wingate
OT  - exercise response
OT  - myoblasts
OT  - myotubes
OT  - skeletal muscle
EDAT- 2023/03/22 06:00
MHDA- 2023/06/05 06:42
CRDT- 2023/03/21 09:23
PHST- 2023/03/07 00:00 [revised]
PHST- 2022/03/29 00:00 [received]
PHST- 2023/03/09 00:00 [accepted]
PHST- 2023/06/05 06:42 [medline]
PHST- 2023/03/22 06:00 [pubmed]
PHST- 2023/03/21 09:23 [entrez]
AID - 10.1111/cpf.12816 [doi]
PST - ppublish
SO  - Clin Physiol Funct Imaging. 2023 Jul;43(4):271-277. doi: 10.1111/cpf.12816. Epub 
      2023 Apr 2.