PMID- 36943045
OWN - NLM
STAT- Publisher
LR  - 20231020
IS  - 2165-0497 (Electronic)
IS  - 2165-0497 (Linking)
VI  - 11
IP  - 2
DP  - 2023 Mar 21
TI  - Discovery of Brassica Yellows Virus and Porcine Reproductive and Respiratory 
      Syndrome Virus in Diaphorina citri and Changes in Virome Due to Infection with 
      'Ca. L. asiaticus'.
PG  - e0499622
LID - 10.1128/spectrum.04996-22 [doi]
LID - e04996-22
AB  - Detection of new viruses or new virus hosts is essential for the protection of 
      economically important agroecosystems and human health. Increasingly, 
      metatranscriptomic data are being used to facilitate this process. Such data were 
      obtained from adult Asian citrus psyllids (ACP) (Diaphorina citri Kuwayama) that 
      fed solely on mandarin (Citrus xaurantium L.) plants grafted with buds infected 
      with 'Candidatus Liberibacter asiaticus' (CLas), a phloem-limited bacterium 
      associated with the severe Asian variant of huanglongbing (HLB), the most 
      destructive disease of citrus. Brassica yellows virus (BrYV), the causative agent 
      of yellowing or leafroll symptoms in brassicaceous plants, and its associated RNA 
      (named as BrYVaRNA) were detected in ACP. In addition, the porcine reproductive 
      and respiratory syndrome virus (PRRSV), which affects pigs and is economically 
      important to pig production, was also found in ACP. These viruses were not 
      detected in insects feeding on plants grafted with CLas-free buds. Changes in the 
      concentrations of insect-specific viruses within the psyllid were caused by 
      coinfection with CLas. IMPORTANCE The cross transmission of pathogenic viruses 
      between different farming systems or plant communities is a major threat to 
      plants and animals and, potentially, human health. The use of metagenomics is an 
      effective approach to discover viruses and vectors. Here, we collected buds from 
      the CLas-infected and CLas-free mandarin (Citrus xaurantium L. [Rutaceae: 
      Aurantioideae: Aurantieae]) trees from a commercial orchard and grafted them onto 
      CLas-free mandarin plants under laboratory conditions. Through metatranscriptome 
      sequencing, we first identified the Asian citrus psyllids feeding on plants 
      grafted with CLas-infected buds carried the plant pathogen, brassica yellows 
      virus and its associated RNA, and the swine pathogen, porcine reproductive and 
      respiratory syndrome virus. These discoveries indicate that both viruses can be 
      transmitted by grafting and acquired by ACP from CLas+ mandarin seedlings.
FAU - Lu, Jinming
AU  - Lu J
AD  - College of Forestry and Biotechnology, Zhejiang A&F University, Linan, Hangzhou, 
      Zhejiang, China.
AD  - College of Plant Protection, South China Agricultural University, Guangzhou, 
      Guangdong, China.
FAU - Zeng, Lixia
AU  - Zeng L
AD  - College of Plant Protection, South China Agricultural University, Guangzhou, 
      Guangdong, China.
FAU - Holford, Paul
AU  - Holford P
AD  - School of Science, Western Sydney University, Penrith, New South Wales, 
      Australia.
FAU - Beattie, George A C
AU  - Beattie GAC
AD  - School of Science, Western Sydney University, Penrith, New South Wales, 
      Australia.
FAU - Wang, Yanjing
AU  - Wang Y
AUID- ORCID: 0000-0003-2931-0910
AD  - The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang 
      Province, College of Advanced Agricultural Sciences, Zhejiang A&F University, 
      Linan, Hangzhou, Zhejiang, China.
LA  - eng
PT  - Journal Article
DEP - 20230321
PL  - United States
TA  - Microbiol Spectr
JT  - Microbiology spectrum
JID - 101634614
SB  - IM
PMC - PMC10100913
OTO - NOTNLM
OT  - Asian citrus psyllid
OT  - brassica yellows virus
OT  - metatranscriptomics
OT  - porcine reproductive and respiratory syndrome
OT  - virus
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/22 06:00
MHDA- 2023/03/22 06:00
PMCR- 2023/03/21
CRDT- 2023/03/21 10:05
PHST- 2023/03/21 10:05 [entrez]
PHST- 2023/03/22 06:00 [pubmed]
PHST- 2023/03/22 06:00 [medline]
PHST- 2023/03/21 00:00 [pmc-release]
AID - 04996-22 [pii]
AID - spectrum.04996-22 [pii]
AID - 10.1128/spectrum.04996-22 [doi]
PST - aheadofprint
SO  - Microbiol Spectr. 2023 Mar 21;11(2):e0499622. doi: 10.1128/spectrum.04996-22.