PMID- 36945463
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230930
DP  - 2023 Mar 8
TI  - An expanded GCaMP reporter toolkit for functional imaging in C. elegans.
LID - 2023.03.06.531342 [pii]
LID - 10.1101/2023.03.06.531342 [doi]
AB  - In living organisms, changes in calcium flux are integral to many different 
      cellular functions and are especially critical for the activity of neurons and 
      myocytes. Genetically encoded calcium indicators (GECIs) have been popular tools 
      for reporting changes in calcium levels in vivo . In particular, GCaMP, derived 
      from GFP, are the most widely used GECIs and have become an invaluable toolkit 
      for neurophysiological studies. Recently, new variants of GCaMP, which offer a 
      greater variety of temporal dynamics and improved brightness, have been 
      developed. However, these variants are not readily available to the 
      Caenorhabditis elegans research community. This work reports a set of GCaMP6 and 
      jGCaMP7 reporters optimized for C. elegans studies. Our toolkit provides 
      reporters with improved dynamic range, varied kinetics, and targeted subcellular 
      localizations. Besides optimized routine uses, this set of reporters are also 
      well-suited for studies requiring fast imaging speeds and low magnification or 
      low-cost platforms.
FAU - Ding, Jimmy
AU  - Ding J
AD  - These authors contributed equally.
AD  - Petit Institute for Bioengineering and Bioscience, Georgia Institute of 
      Technology, Atlanta, GA 30332, USA.
FAU - Peng, Lucinda
AU  - Peng L
AD  - These authors contributed equally.
AD  - Petit Institute for Bioengineering and Bioscience, Georgia Institute of 
      Technology, Atlanta, GA 30332, USA.
FAU - Moon, Sihoon
AU  - Moon S
AD  - These authors contributed equally.
AD  - School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 
      Atlanta, GA 30332, USA.
FAU - Lee, Hyun Jee
AU  - Lee HJ
AD  - These authors contributed equally.
AD  - School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 
      Atlanta, GA 30332, USA.
FAU - Patel, Dhaval S
AU  - Patel DS
AD  - School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 
      Atlanta, GA 30332, USA.
AD  - Present address: NemaLife Inc, Lubbock, TX 79409, USA.
FAU - Lu, Hang
AU  - Lu H
AD  - Petit Institute for Bioengineering and Bioscience, Georgia Institute of 
      Technology, Atlanta, GA 30332, USA.
AD  - School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, 
      Atlanta, GA 30332, USA.
LA  - eng
GR  - R01 AG056436/AG/NIA NIH HHS/United States
GR  - R01 NS096581/NS/NINDS NIH HHS/United States
GR  - R01 NS115484/NS/NINDS NIH HHS/United States
PT  - Preprint
DEP - 20230308
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
UIN - G3 (Bethesda). 2023 Aug 11;:. PMID: 37565483
PMC - PMC10028802
EDAT- 2023/03/23 06:00
MHDA- 2023/03/23 06:01
PMCR- 2023/03/21
CRDT- 2023/03/22 01:57
PHST- 2023/03/22 01:57 [entrez]
PHST- 2023/03/23 06:00 [pubmed]
PHST- 2023/03/23 06:01 [medline]
PHST- 2023/03/21 00:00 [pmc-release]
AID - 2023.03.06.531342 [pii]
AID - 10.1101/2023.03.06.531342 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2023 Mar 8:2023.03.06.531342. doi: 10.1101/2023.03.06.531342.