PMID- 36945523
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230608
DP  - 2023 Mar 10
TI  - High fidelity sensory-evoked responses in neocortex after intravenous injection 
      of genetically encoded calcium sensors.
LID - 2023.03.09.531938 [pii]
LID - 10.1101/2023.03.09.531938 [doi]
AB  - Two-photon imaging of genetically-encoded calcium indicators (GECIs) has 
      traditionally relied on intracranial injections of adeno-associated virus (AAV) 
      or transgenic animals to achieve expression. Intracranial injections require an 
      invasive surgery and result in a relatively small volume of tissue labeling. 
      Transgenic animals, although they can have brain-wide GECI expression, often 
      express GECIs in only a small subset of neurons, may have abnormal behavioral 
      phenotypes, and are currently limited to older generations of GECIs. Inspired by 
      recent developments in the synthesis of AAVs that readily cross the blood brain 
      barrier, we tested whether an alternative strategy of intravenously injecting 
      AAV-PhP.eB is suitable for two-photon calcium imaging of neurons over many months 
      after injection. We injected young (postnatal day 23 to 31) C57BL/6J mice with 
      AAV-PhP.eB-Synapsin-jGCaMP7s via the retro-orbital sinus. After allowing 5 to 34 
      weeks for expression, we performed conventional and widefield two-photon imaging 
      of layers 2/3, 4 and 5 of the primary visual cortex. We found reproducible 
      trial-by-trial neural responses and tuning properties consistent with known 
      feature selectivity in the visual cortex. Thus, intravenous injection of 
      AAV-PhP.eB does not interfere with the normal processing in neural circuits. In 
      vivo and histological images show no nuclear expression of jGCaMP7s for at least 
      34 weeks post-injection.
FAU - Leikvoll, Austin
AU  - Leikvoll A
AD  - Department of Neuroscience, University of Minnesota, Minneapolis MN.
FAU - Kara, Prakash
AU  - Kara P
AD  - Department of Neuroscience, University of Minnesota, Minneapolis MN.
LA  - eng
PT  - Preprint
DEP - 20230310
PL  - United States
TA  - bioRxiv
JT  - bioRxiv : the preprint server for biology
JID - 101680187
UIN - Front Neurosci. 2023 May 12;17:1181828. PMID: 37250396
PMC - PMC10028972
COIS- Conflict of Interest The authors declare that the research was conducted in the 
      absence of any commercial or financial relationships that could be construed as a 
      potential conflict of interest.
EDAT- 2023/03/23 06:00
MHDA- 2023/03/23 06:01
PMCR- 2023/03/21
CRDT- 2023/03/22 01:57
PHST- 2023/03/22 01:57 [entrez]
PHST- 2023/03/23 06:00 [pubmed]
PHST- 2023/03/23 06:01 [medline]
PHST- 2023/03/21 00:00 [pmc-release]
AID - 2023.03.09.531938 [pii]
AID - 10.1101/2023.03.09.531938 [doi]
PST - epublish
SO  - bioRxiv [Preprint]. 2023 Mar 10:2023.03.09.531938. doi: 
      10.1101/2023.03.09.531938.