PMID- 36946032
OWN - NLM
STAT- MEDLINE
DCOM- 20231121
LR  - 20231121
IS  - 1475-2662 (Electronic)
IS  - 0007-1145 (Linking)
VI  - 130
IP  - 10
DP  - 2023 Nov 28
TI  - Comparative transcriptomic analysis of mammary gland tissues reveals the critical 
      role of GPR110 in palmitic acid-stimulated milk protein and fat synthesis.
PG  - 1665-1677
LID - 10.1017/S0007114523000788 [doi]
AB  - The G protein-coupled receptors (GPCR) sensing nutritional signals (amino acids, 
      fatty acids, glucose, etc.) are not fully understood. In this research, we used 
      transcriptome sequencing to analyse differentially expressed genes (DEG) in mouse 
      mammary gland tissues at puberty, lactation and involution stages, in which eight 
      GPCR were selected out and verified by qRT-PCR assay. It was further identified 
      the role of GPR110-mediating nutrients including palmitic acid (PA) and 
      methionine (Met) to improve milk synthesis using mouse mammary epithelial cell 
      line HC11. PA but not Met affected GPR110 expression in a dose-dependent manner. 
      GPR110 knockdown decreased milk protein and fat synthesis and cell proliferation 
      and blocked the stimulation of PA on mechanistic target of rapamycin (mTOR) 
      phosphorylation and sterol-regulatory element binding protein 1c (SREBP-1c) 
      expression. In summary, these experimental results disclose DEG related to 
      lactation and reveal that GPR110 mediates PA to activate the mTOR and SREBP-1c 
      pathways to promote milk protein and fat synthesis.
FAU - Zhang, Minghui
AU  - Zhang M
AUID- ORCID: 0000-0002-8253-1379
AD  - College of Animal Science, Yangtze University, Jingmi Road 88, Jingzhou434025, 
      People's Republic of China.
FAU - Ma, Zonghua
AU  - Ma Z
AD  - College of Animal Science, Yangtze University, Jingmi Road 88, Jingzhou434025, 
      People's Republic of China.
FAU - Qi, Hao
AU  - Qi H
AUID- ORCID: 0000-0003-0059-5377
AD  - College of Animal Science, Yangtze University, Jingmi Road 88, Jingzhou434025, 
      People's Republic of China.
FAU - Cui, Xu
AU  - Cui X
AD  - College of Animal Science, Yangtze University, Jingmi Road 88, Jingzhou434025, 
      People's Republic of China.
FAU - Li, Rui
AU  - Li R
AD  - College of Animal Science, Yangtze University, Jingmi Road 88, Jingzhou434025, 
      People's Republic of China.
FAU - Gao, Xuejun
AU  - Gao X
AUID- ORCID: 0000-0002-8241-6346
AD  - College of Animal Science, Yangtze University, Jingmi Road 88, Jingzhou434025, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230322
PL  - England
TA  - Br J Nutr
JT  - The British journal of nutrition
JID - 0372547
RN  - AE28F7PNPL (Methionine)
RN  - 0 (Milk Proteins)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (ADGRF1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Female
MH  - Mice
MH  - Epithelial Cells/metabolism
MH  - *Lactation/genetics/metabolism
MH  - *Mammary Glands, Animal/drug effects/metabolism
MH  - Methionine/metabolism
MH  - *Milk Proteins/metabolism
MH  - Palmitic Acid/pharmacology
MH  - Receptors, G-Protein-Coupled/genetics
MH  - Sexual Maturation
MH  - Sterol Regulatory Element Binding Protein 1/genetics/metabolism
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
MH  - Transcriptome
OTO - NOTNLM
OT  - GPR110
OT  - Milk fat synthesis
OT  - Milk protein synthesis
OT  - Palmitic acid
OT  - Transcriptomic analysis
EDAT- 2023/03/23 06:00
MHDA- 2023/10/23 00:43
CRDT- 2023/03/22 03:53
PHST- 2023/10/23 00:43 [medline]
PHST- 2023/03/23 06:00 [pubmed]
PHST- 2023/03/22 03:53 [entrez]
AID - S0007114523000788 [pii]
AID - 10.1017/S0007114523000788 [doi]
PST - ppublish
SO  - Br J Nutr. 2023 Nov 28;130(10):1665-1677. doi: 10.1017/S0007114523000788. Epub 
      2023 Mar 22.