PMID- 36946983
OWN - NLM
STAT- MEDLINE
DCOM- 20230324
LR  - 20230505
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 220
IP  - 6
DP  - 2023 Jun 5
TI  - CCL5-producing migratory dendritic cells guide CCR5+ monocytes into the draining 
      lymph nodes.
LID - 10.1084/jem.20222129 [doi]
LID - e20222129
AB  - Dendritic cells (DCs) and monocytes capture, transport, and present antigen to 
      cognate T cells in the draining lymph nodes (LNs) in a CCR7-dependent manner. 
      Since only migratory DCs express this chemokine receptor, it is unclear how 
      monocytes reach the LN. In steady-state and following inhalation of several 
      PAMPs, scRNA-seq identified LN mononuclear phagocytes as monocytes, resident, or 
      migratory type 1 and type 2 conventional (c)DCs, despite the downregulation of 
      Xcr1, Clec9a, H2-Ab1, Sirpa, and Clec10a transcripts on migratory cDCs. Migratory 
      cDCs, however, upregulated Ccr7, Ccl17, Ccl22, and Ccl5. Migratory monocytes 
      expressed Ccr5, a high-affinity receptor for Ccl5. Using two tracking methods, we 
      observed that both CD88hiCD26lomonocytes and CD88-CD26hi cDCs captured inhaled 
      antigens in the lung and migrated to LNs. Antigen exposure in mixed-chimeric 
      Ccl5-, Ccr2-, Ccr5-, Ccr7-, and Batf3-deficient mice demonstrated that while 
      antigen-bearing DCs use CCR7 to reach the LN, monocytes use CCR5 to follow 
      CCL5-secreting migratory cDCs into the LN, where they regulate DC-mediated 
      immunity.
CI  - (c) 2023 Rawat et al.
FAU - Rawat, Kavita
AU  - Rawat K
AUID- ORCID: 0000-0002-8302-9890
AD  - Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth 
      , Hanover, NH, USA.
FAU - Tewari, Anita
AU  - Tewari A
AUID- ORCID: 0000-0001-6331-1701
AD  - Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth 
      , Hanover, NH, USA.
FAU - Li, Xin
AU  - Li X
AUID- ORCID: 0000-0002-2050-2628
AD  - Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth 
      , Hanover, NH, USA.
FAU - Mara, Arlind B
AU  - Mara AB
AUID- ORCID: 0000-0001-9823-5243
AD  - Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth 
      , Hanover, NH, USA.
FAU - King, William T
AU  - King WT
AUID- ORCID: 0000-0003-2312-6207
AD  - Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth 
      , Hanover, NH, USA.
FAU - Gibbings, Sophie L
AU  - Gibbings SL
AUID- ORCID: 0000-0001-9623-0705
AD  - Department of Pediatrics, National Jewish Health , Denver, CO, USA.
FAU - Nnam, Chinaza F
AU  - Nnam CF
AUID- ORCID: 0000-0001-6230-6169
AD  - Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth 
      , Hanover, NH, USA.
FAU - Kolling, Fred W
AU  - Kolling FW
AUID- ORCID: 0000-0002-6178-9901
AD  - Dartmouth Cancer Center, Dartmouth Geisel School of Medicine , Lebanon, NH, USA.
FAU - Lambrecht, Bart N
AU  - Lambrecht BN
AUID- ORCID: 0000-0003-4376-6834
AD  - Laboratory of Immunoregulation and Mucosal Immunology, VIB-UGent Center for 
      Inflammation Research , Ghent, Belgium.
AD  - Department of Internal Medicine and Pediatrics, Ghent University , Ghent, 
      Belgium.
AD  - Department of Pulmonary Medicine, Erasmus MC , Rotterdam, Netherlands.
FAU - Jakubzick, Claudia V
AU  - Jakubzick CV
AUID- ORCID: 0000-0002-3731-0198
AD  - Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth 
      , Hanover, NH, USA.
LA  - eng
GR  - R01 HL135001/HL/NHLBI NIH HHS/United States
GR  - S10 OD025235/OD/NIH HHS/United States
GR  - R01 HL115334/HL/NHLBI NIH HHS/United States
GR  - S10 OD030242/OD/NIH HHS/United States
GR  - P20 GM130454/GM/NIGMS NIH HHS/United States
GR  - P30 CA023108/CA/NCI NIH HHS/United States
GR  - R35 HL155458/HL/NHLBI NIH HHS/United States
GR  - 789384/ERC_/European Research Council/International
GR  - T32 AI007363/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20230322
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Receptors, CCR7)
RN  - 0 (Antigens)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Monocytes
MH  - *Dendritic Cells
MH  - Receptors, CCR7
MH  - Lung
MH  - Antigens
MH  - Lymph Nodes
MH  - Cell Movement
MH  - Mice, Inbred C57BL
PMC - PMC10072223
COIS- Disclosures: The authors declare no competing interests exist.
EDAT- 2023/03/23 06:00
MHDA- 2023/03/25 06:00
PMCR- 2023/03/22
CRDT- 2023/03/22 11:02
PHST- 2022/12/12 00:00 [received]
PHST- 2023/02/10 00:00 [revised]
PHST- 2023/03/08 00:00 [accepted]
PHST- 2023/03/22 11:02 [entrez]
PHST- 2023/03/23 06:00 [pubmed]
PHST- 2023/03/25 06:00 [medline]
PHST- 2023/03/22 00:00 [pmc-release]
AID - 213962 [pii]
AID - jem.20222129 [pii]
AID - 10.1084/jem.20222129 [doi]
PST - ppublish
SO  - J Exp Med. 2023 Jun 5;220(6):e20222129. doi: 10.1084/jem.20222129. Epub 2023 Mar 
      22.