PMID- 36947320 OWN - NLM STAT- MEDLINE DCOM- 20230529 LR - 20240323 IS - 2212-5469 (Electronic) IS - 1738-2696 (Print) IS - 1738-2696 (Linking) VI - 20 IP - 3 DP - 2023 Jun TI - Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells. PG - 447-459 LID - 10.1007/s13770-023-00531-2 [doi] AB - BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton's jelly. Subsequently, hUCMSCs were exposed to cold storage (4 degrees C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2-related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs. CI - (c) 2023. Korean Tissue Engineering and Regenerative Medicine Society. FAU - Zhang, Ying AU - Zhang Y AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Wang, Peng AU - Wang P AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Jin, Mei-Xian AU - Jin MX AD - Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Zhou, Ying-Qi AU - Zhou YQ AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Ye, Liang AU - Ye L AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Zhu, Xiao-Juan AU - Zhu XJ AD - Department of Anesthesiology, First People's Hospital of Kashi, Kashi, 844000, China. FAU - Li, Hui-Fang AU - Li HF AD - Department of Anesthesiology, First People's Hospital of Kashi, Kashi, 844000, China. FAU - Zhou, Ming AU - Zhou M AD - Department of Anesthesiology, First People's Hospital of Kashi, Kashi, 844000, China. FAU - Li, Yang AU - Li Y AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Li, Shao AU - Li S AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Liang, Kang-Yan AU - Liang KY AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Wang, Yi AU - Wang Y AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Gao, Yi AU - Gao Y AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Pan, Ming-Xin AU - Pan MX AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Zhou, Shu-Qin AU - Zhou SQ AD - Department of Anesthesiology, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. zhoushuqin@smu.edu.cn. FAU - Peng, Qing AU - Peng Q AUID- ORCID: 0000-0002-7790-6455 AD - General Surgery Center, Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. zhujiangzhuanhua@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20230322 PL - Korea (South) TA - Tissue Eng Regen Med JT - Tissue engineering and regenerative medicine JID - 101699923 RN - 02XA4X3KZW (schizandrin B) RN - 0 (Celsior) RN - 0 (Lignans) SB - IM MH - Humans MH - *Mesenchymal Stem Cells/metabolism MH - *Lignans/pharmacology/metabolism MH - Umbilical Cord PMC - PMC10219924 OTO - NOTNLM OT - Low temperature preservation OT - Schisandrin B OT - Stem cells COIS- The authors declare that there are no conflicts of interest. EDAT- 2023/03/23 06:00 MHDA- 2023/05/29 06:42 PMCR- 2024/03/22 CRDT- 2023/03/22 12:20 PHST- 2022/12/01 00:00 [received] PHST- 2023/02/17 00:00 [accepted] PHST- 2023/02/14 00:00 [revised] PHST- 2023/05/29 06:42 [medline] PHST- 2023/03/23 06:00 [pubmed] PHST- 2023/03/22 12:20 [entrez] PHST- 2024/03/22 00:00 [pmc-release] AID - 10.1007/s13770-023-00531-2 [pii] AID - 531 [pii] AID - 10.1007/s13770-023-00531-2 [doi] PST - ppublish SO - Tissue Eng Regen Med. 2023 Jun;20(3):447-459. doi: 10.1007/s13770-023-00531-2. Epub 2023 Mar 22.