PMID- 36947736
OWN - NLM
STAT- MEDLINE
DCOM- 20230406
LR  - 20230406
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 71
IP  - 13
DP  - 2023 Apr 5
TI  - Identification of Scoparone from Chinese Olive Fruit as a Modulator of Macrophage 
      Polarization.
PG  - 5195-5207
LID - 10.1021/acs.jafc.2c08132 [doi]
AB  - Chinese olive (Canarium album L.) has been highlighted for its remarkable health 
      benefits. We previously showed that the ethyl acetate fraction of Chinese olive 
      (COE) is an effective anti-inflammatory agent. In this study, we used a 
      luciferase-based RAW 264.7 cell platform to detect the transcriptional activity 
      of NF-kappaB, a key mediator of inflammation, and the promoter activity of its 
      downstream target, COX-2. Through functional-oriented screening using these 
      platforms, we further divided COE into several subfractions. Subsequently, we 
      used silica gel column chromatography for purification, and the active compounds 
      were separated and isolated by thin-layer chromatography (TLC) and 
      high-performance liquid chromatography (HPLC). The structure of the resulting 
      compound with high anti-inflammatory activity was then identified as scoparone. 
      Our results showed that scoparone not only inhibited lipopolysaccharide 
      (LPS)-induced secretion of nitric oxide and suppressed M1 macrophage markers 
      (iNOS, Il-6, Ccl2, and Tnf-alpha) but also markedly decreased the production of 
      pro-inflammatory cytokines (IL-6, CCL2, and TNF-alpha). Treatment with scoparone 
      significantly reduced the protein level of TNF-alpha in LPS-treated 
      bone-marrow-derived macrophages (BMDMs). In addition, scoparone promoted 
      macrophages toward an M2 anti-inflammatory phenotype, as determined by the 
      significantly increased gene expression of M2 macrophage markers (Arg1, Ym1, 
      Mrc1, Il-10, and Cd206) and the protein level of Arg1. This study indicates that 
      COE fruit has high therapeutic potential for various inflammatory diseases as a 
      result of switching the macrophage phenotype from pro-inflammatory M1 to 
      anti-inflammatory M2.
FAU - Yeh, Yu-Te
AU  - Yeh YT
AUID- ORCID: 0000-0001-7968-6081
AD  - Institute of Food Science and Technology, National Taiwan University, Taipei 106, 
      Taiwan.
AD  - Division of Endocrinology, Diabetes, and Nutrition, Department of Medicine, 
      University of Maryland School of Medicine, Baltimore, Maryland 21201, United 
      States.
FAU - Hsu, Kuei-Ming
AU  - Hsu KM
AD  - Institute of Food Science and Technology, National Taiwan University, Taipei 106, 
      Taiwan.
FAU - Chen, Hong-Jhang
AU  - Chen HJ
AUID- ORCID: 0000-0002-9420-0964
AD  - Institute of Food Science and Technology, National Taiwan University, Taipei 106, 
      Taiwan.
FAU - Su, Nan-Wei
AU  - Su NW
AUID- ORCID: 0000-0002-1402-7349
AD  - Laboratory of Food Chemistry, Department of Agricultural Chemistry, National 
      Taiwan University, Taipei 106, Taiwan.
FAU - Liao, Yi-Chun
AU  - Liao YC
AD  - Department of Biochemical Science and Technology, National Taiwan University, 
      Taipei 106, Taiwan.
FAU - Hsieh, Shu-Chen
AU  - Hsieh SC
AD  - Institute of Food Science and Technology, National Taiwan University, Taipei 106, 
      Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20230322
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - H5841PDT4Y (scoparone)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Coumarins)
SB  - IM
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Fruit/chemistry
MH  - Inflammation/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - *Macrophages/drug effects
MH  - NF-kappa B/metabolism
MH  - *Tumor Necrosis Factor-alpha/metabolism
MH  - Animals
MH  - Mice
MH  - RAW 264.7 Cells
MH  - *Coumarins/pharmacology
OTO - NOTNLM
OT  - Chinese olive
OT  - inflammation
OT  - luciferase assay
OT  - macrophage
OT  - scoparone
EDAT- 2023/03/23 06:00
MHDA- 2023/04/06 06:41
CRDT- 2023/03/22 16:03
PHST- 2023/04/06 06:41 [medline]
PHST- 2023/03/23 06:00 [pubmed]
PHST- 2023/03/22 16:03 [entrez]
AID - 10.1021/acs.jafc.2c08132 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2023 Apr 5;71(13):5195-5207. doi: 10.1021/acs.jafc.2c08132. 
      Epub 2023 Mar 22.