PMID- 36948037
OWN - NLM
STAT- MEDLINE
DCOM- 20230501
LR  - 20230501
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 102
IP  - 5
DP  - 2023 May
TI  - Effects of thermal stress and mechanistic target of rapamycin and wingless-type 
      mouse mammary tumor virus integration site family pathways on the proliferation 
      and differentiation of satellite cells derived from the breast muscle of 
      different chicken lines.
PG  - 102608
LID - S0032-5791(23)00132-3 [pii]
LID - 10.1016/j.psj.2023.102608 [doi]
LID - 102608
AB  - Satellite cells (SCs) are muscle stem cells responsible for muscle hypertrophic 
      growth and the regeneration of damaged muscle. Proliferation and differentiation 
      of the pectoralis major (p. major) muscle SCs are responsive to thermal stress in 
      turkeys, which are, in part, regulated by mechanistic target of rapamycin (mTOR) 
      and Frizzled7 (Fzd7)-mediated wingless-type mouse mammary tumor virus integration 
      site family/planar cell polarity (Wnt/PCP) pathways in a growth dependent-manner. 
      It is not known if chicken p. major SCs respond to thermal stress in a manner 
      similar to that of turkey p. major SCs. The objective of the current study was to 
      investigate the effects of thermal stress and mTOR and Wnt/PCP pathways on the 
      proliferation, differentiation, and expression of myogenic transcriptional 
      regulatory factors in SCs isolated from the p. major muscle of a current modern 
      commercial (MC) broiler line as compared to that of a Cornish Rock (BPM8) and 
      Randombred (RBch) chicken line in the 1990s. The MC line SCs had lower 
      proliferation and differentiation rates and decreased expression of myoblast 
      determination factor 1 (MyoD) and myogenin (MyoG) compared to the BPM8 and RBch 
      lines. Heat stress (43 degrees C) increased proliferation and MyoD expression in all the 
      cell lines, while cold stress (33 degrees C) showed a suppressive effect compared to the 
      control temperature (38 degrees C). Satellite cell differentiation was altered with heat 
      and cold stress in a cell line-specific manner. In general, the differentiation 
      of the MC SCs was less responsive to both heat and cold stress compared to the 
      BPM8 and RBch lines. Knockdown of the expression of either mTOR or Fzd7 decreased 
      the proliferation, differentiation, and the expression of MyoD and MyoG in all 
      the cell lines. The MC line during proliferation was more dependent on the 
      expression of mTOR and Fzd7 than during differentiation. Thus, modern commercial 
      meat-type chickens have decreased myogenic activity and temperature sensitivity 
      of SCs in an mTOR- and Fzd7-dependent manner. The decrease in muscle regeneration 
      will make modern commercial broilers more susceptible to the negative effects of 
      myopathies with muscle fiber necrosis requiring satellite cell-mediated repair.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Xu, Jiahui
AU  - Xu J
AD  - Department of Animal Sciences, The Ohio State University, Wooster, OH 44691, USA.
FAU - Velleman, Sandra G
AU  - Velleman SG
AD  - Department of Animal Sciences, The Ohio State University, Wooster, OH 44691, USA. 
      Electronic address: velleman.1@osu.edu.
LA  - eng
PT  - Journal Article
DEP - 20230228
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - 0 (Myogenic Regulatory Factors)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Chickens/physiology
MH  - Pectoralis Muscles
MH  - Mammary Tumor Virus, Mouse
MH  - Cells, Cultured
MH  - Cell Proliferation
MH  - Cell Differentiation
MH  - TOR Serine-Threonine Kinases/metabolism
MH  - Myogenic Regulatory Factors/metabolism
MH  - *Satellite Cells, Skeletal Muscle/metabolism
MH  - Muscle, Skeletal/physiology
PMC - PMC10033751
OTO - NOTNLM
OT  - Wnt
OT  - chicken
OT  - mTOR
OT  - satellite cell
OT  - thermal stress
EDAT- 2023/03/23 06:00
MHDA- 2023/05/01 06:42
PMCR- 2023/02/28
CRDT- 2023/03/22 19:06
PHST- 2022/12/05 00:00 [received]
PHST- 2023/01/07 00:00 [revised]
PHST- 2023/02/17 00:00 [accepted]
PHST- 2023/05/01 06:42 [medline]
PHST- 2023/03/23 06:00 [pubmed]
PHST- 2023/03/22 19:06 [entrez]
PHST- 2023/02/28 00:00 [pmc-release]
AID - S0032-5791(23)00132-3 [pii]
AID - 102608 [pii]
AID - 10.1016/j.psj.2023.102608 [doi]
PST - ppublish
SO  - Poult Sci. 2023 May;102(5):102608. doi: 10.1016/j.psj.2023.102608. Epub 2023 Feb 
      28.