PMID- 36951077
OWN - NLM
STAT- MEDLINE
DCOM- 20230324
LR  - 20230324
IS  - 1000-0607 (Print)
IS  - 1000-0607 (Linking)
VI  - 48
IP  - 3
DP  - 2023 Mar 25
TI  - [Effect of moxibustion on the indicators of autophagy and apoptosis in synovium 
      of rats with adjuvant arthritis].
PG  - 253-8
LID - 10.13702/j.1000-0607.20220959 [doi]
AB  - OBJECTIVE: To observe the effect of moxibustion on the indicators of autophagy 
      and apoptosis in the synovium of toes of rats with adjuvant-induced arthritis 
      (AA), so as to explore the underlying mechanism of moxibustion in the treatment 
      of rheumatoid arthritis. METHODS: Forty-five SD rats were randomly divided into 
      the blank control group, model group, moxibustion group, methotrexate group and 
      rapamycin group, with 9 rats in each group. The rat model of AA was established 
      by injecting Freund's complete adjuvant. Rats in the moxibustion group received 
      moxibustion treatment at "Zusanli" (ST36) and "Guanyuan" (CV4) for 20 min, once a 
      day. The methotrexate group was given methotrexate intragastrically (0.35 mg/kg) 
      twice a week. The rapamycin group was given rapamycin by intraperitoneal 
      injection (1 mg/kg), once every other day. The toe volume of the left hind limb 
      was measured by the toe volume measuring instrument after 3-day modeling and 
      3-week intervention respectively. The contents of interlukin(IL)-1 and tumor 
      necrosis factor(TNF)-alpha in serum were detected by ELISA. The autophagosomes of 
      synovial cells of the toe joint were observed under transmission electron 
      microscope. The expressions of mammalian target of rapamycin(mTOR)C1, p-mTORC1, 
      Caspase-3, Fas and FasL in synovial tissue were detected by Western blot. 
      RESULTS: Under transmission electron microscope, the model group showed decreased 
      autophagosomes in synovial tissues, but the moxibustion, methotrexate, and 
      rapamycin groups showed increased autophagosomes. Compared with the blank control 
      group, the toe volume, the contents of IL-1 and TNF-alpha in serum and the expression 
      of p-mTORC1 protein in synovial tissue were significantly increased (P<0.01, 
      P<0.001), while the expressions of Caspase-3, Fas and FasL proteins in synovial 
      tissue were significantly decreased (P<0.05, P<0.01) in the model group. Compared 
      with the model group, the toe volume, the contents of IL-1 and TNF-alpha in the 
      serum, and expression of p-mTORC1 protein were significantly decreased (P<0.05, 
      P<0.01, P<0.001) in the moxibustion group and the methotrexate group, while the 
      expression of Caspase-3, Fas and FasL proteins in synovial tissue in the 
      moxibustion group and the methotrexate group, the expression of Caspase-3 in the 
      rapamycin group were significantly increased (P<0.05). CONCLUSION: Moxibustion 
      can improve joint swelling in AA rats and decrease the contents of serum IL-1 and 
      TNF-alpha. The mechanism may be related to regulating the expressions of p-mTORC1, 
      Caspase-3, Fas and FasL proteins, and promoting autophagy and apoptosis of 
      synovial cells.
FAU - Liu, Li
AU  - Liu L
AD  - The First Affiliated Hospital /The First Clinical College of Hunan University of 
      Chinese Medicine, Changsha 410007, China.
FAU - Zhang, Liang
AU  - Zhang L
AD  - College of Acupuncture-Moxibustion and Tuina, Hunan University of Chinese 
      Medicine, Changsha 410208.
FAU - Ai, Kun
AU  - Ai K
AD  - College of Acupuncture-Moxibustion and Tuina, Hunan University of Chinese 
      Medicine, Changsha 410208.
FAU - Qi, Fang
AU  - Qi F
AD  - College of Acupuncture-Moxibustion and Tuina, Hunan University of Chinese 
      Medicine, Changsha 410208.
FAU - Li, Xin
AU  - Li X
AD  - Hunan Provincial Key Laboratory of Diagnostics of Chinese Medicine, Hunan 
      University of Chinese Medicine, Changsha 410208.
FAU - Wang, Wen-Yi
AU  - Wang WY
AD  - College of Nursing, Hunan University of Chinese Medicine, Changsha 410208.
FAU - Xu, Mei-Li
AU  - Xu ML
AD  - College of Nursing, Hunan University of Chinese Medicine, Changsha 410208.
FAU - Gong, Zhi-Xian
AU  - Gong ZX
AD  - The First Affiliated Hospital /The First Clinical College of Hunan University of 
      Chinese Medicine, Changsha 410007, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhen Ci Yan Jiu
JT  - Zhen ci yan jiu = Acupuncture research
JID - 8507710
RN  - EC 3.4.22.- (Caspase 3)
RN  - YL5FZ2Y5U1 (Methotrexate)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Interleukin-1)
SB  - IM
MH  - Rats
MH  - Animals
MH  - *Moxibustion
MH  - Caspase 3/genetics/metabolism
MH  - Rats, Sprague-Dawley
MH  - *Arthritis, Experimental/genetics/therapy
MH  - Methotrexate/metabolism/pharmacology
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Synovial Membrane/metabolism
MH  - Apoptosis
MH  - Autophagy
MH  - Interleukin-1/metabolism/pharmacology
MH  - Mammals
OTO - NOTNLM
OT  - Apoptosis
OT  - Autophagy
OT  - Moxibustion
OT  - Rheumatoid arthritis
OT  - Synovial cells
EDAT- 2023/03/24 06:00
MHDA- 2023/03/25 06:00
CRDT- 2023/03/23 05:03
PHST- 2023/03/23 05:03 [entrez]
PHST- 2023/03/24 06:00 [pubmed]
PHST- 2023/03/25 06:00 [medline]
AID - 10.13702/j.1000-0607.20220959 [doi]
PST - ppublish
SO  - Zhen Ci Yan Jiu. 2023 Mar 25;48(3):253-8. doi: 10.13702/j.1000-0607.20220959.