PMID- 36951591
OWN - NLM
STAT- MEDLINE
DCOM- 20230331
LR  - 20230401
IS  - 1939-1676 (Electronic)
IS  - 0891-6640 (Print)
IS  - 0891-6640 (Linking)
VI  - 37
IP  - 2
DP  - 2023 Mar
TI  - Circulating neutrophil extracellular traps in cats with hypertrophic 
      cardiomyopathy and cardiogenic arterial thromboembolism.
PG  - 490-502
LID - 10.1111/jvim.16676 [doi]
AB  - BACKGROUND: Cats with hypertrophic cardiomyopathy (HCM) are at risk of 
      cardiogenic arterial thromboembolism (CATE). Neutrophil extracellular traps 
      (NETs) may be a potential biomarker and therapeutic target for cardiomyopathy in 
      cats. HYPOTHESIS/OBJECTIVES: Characterize NETs in cats with HCM or CATE. We 
      hypothesized that circulating NETs assessed in the form of cell-free DNA (cfDNA) 
      and citrullinated histone H3 (citH3) are increased in cats with HCM and CATE and 
      associated with reported predisposing factors for thrombus formation. ANIMALS: 
      Eighty-five cats including client-owned cats with HCM and CATE and staff- and 
      student-owned clinically healthy cats without HCM. METHODS: After 
      echocardiographic evaluations, NETs were measured as cfDNA and citH3. RESULTS: 
      Cats with CATE had significant increases in cfDNA (11.2 ng/muL; interquartile 
      range [IQR], 8.1 to 29.6) compared to those without HCM (8.2 ng/muL; IQR, 5.7 to 
      11.7 muL; P = .01) and were responsible for 75% to 83% of cases with cfDNA 
      fragments sized 100 to 2000 base pairs. Citrullinated histone 3, detected in 52% 
      of cats with HCM (31.1 ng/mL; IQR, 16.9 to 29.8), was significantly lower than in 
      those with CATE (48.2 ng/mL; IQR, 34.2 to 60.2; P = .007). The citH3 
      concentrations correlated significantly with reported risk factors of CATE, such 
      as left atrial auricular velocity. CONCLUSIONS AND CLINICAL IMPORTANCE: 
      Neutrophil extracellualr traps, especially citH3, are increased in cats with HCM 
      and CATE. They may serve as a novel therapeutic target and biomarker of 
      thrombosis in cats with HCM.
CI  - (c) 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley 
      Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
FAU - Li, Ronald H L
AU  - Li RHL
AUID- ORCID: 0000-0002-3325-2744
AD  - Department of Surgical and Radiological Sciences, School of Veterinary Medicine, 
      University of California, Davis, Davis, California, USA.
FAU - Fabella, Arianne
AU  - Fabella A
AD  - Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University 
      of California, Davis, Davis, California, USA.
FAU - Nguyen, Nghi
AU  - Nguyen N
AD  - Department of Surgical and Radiological Sciences, School of Veterinary Medicine, 
      University of California, Davis, Davis, California, USA.
FAU - Kaplan, Joanna L
AU  - Kaplan JL
AD  - Department of Medicine and Epidemiology, School of Veterinary Medicine, 
      University of California, Davis, Davis, California, USA.
FAU - Ontiveros, Eric
AU  - Ontiveros E
AD  - Department of Medicine and Epidemiology, School of Veterinary Medicine, 
      University of California, Davis, Davis, California, USA.
FAU - Stern, Joshua A
AU  - Stern JA
AD  - Department of Medicine and Epidemiology, School of Veterinary Medicine, 
      University of California, Davis, Davis, California, USA.
LA  - eng
GR  - D20FE-805/Morris Animal Foundation/
PT  - Journal Article
DEP - 20230323
PL  - United States
TA  - J Vet Intern Med
JT  - Journal of veterinary internal medicine
JID - 8708660
RN  - 0 (Histones)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Cats
MH  - Animals
MH  - *Extracellular Traps
MH  - Neutrophils
MH  - Histones
MH  - Biomarkers
MH  - *Cardiomyopathy, Hypertrophic/veterinary
MH  - *Thromboembolism/veterinary
MH  - *Cat Diseases
PMC - PMC10061180
OTO - NOTNLM
OT  - NETosis
OT  - cell-free DNA
OT  - fragment size
OT  - peptidyl arginine deiminase 4
COIS- Johaua A Stern serves as Associate Editor for the Journal of Veterinary Internal 
      Medicine. He was not involved in review of this manuscript. No other authors 
      declare a conflict of interest.
EDAT- 2023/03/24 06:00
MHDA- 2023/03/31 06:42
PMCR- 2023/03/23
CRDT- 2023/03/23 10:07
PHST- 2022/04/04 00:00 [received]
PHST- 2023/02/14 00:00 [accepted]
PHST- 2023/03/31 06:42 [medline]
PHST- 2023/03/24 06:00 [pubmed]
PHST- 2023/03/23 10:07 [entrez]
PHST- 2023/03/23 00:00 [pmc-release]
AID - JVIM16676 [pii]
AID - 10.1111/jvim.16676 [doi]
PST - ppublish
SO  - J Vet Intern Med. 2023 Mar;37(2):490-502. doi: 10.1111/jvim.16676. Epub 2023 Mar 
      23.