PMID- 36967852
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230328
IS  - 1734-9338 (Print)
IS  - 1897-4295 (Electronic)
IS  - 1734-9338 (Linking)
VI  - 18
IP  - 4
DP  - 2022 Dec
TI  - Extracellular vesicles derived from human umbilical cord mesenchymal stem cells 
      stimulate angiogenesis in myocardial infarction via the microRNA-423-5p/EFNA3 
      axis.
PG  - 373-391
LID - 10.5114/aic.2023.124797 [doi]
AB  - INTRODUCTION: Myocardial infarction (MI) is a severe disease that has an 
      association with angiogenesis dysfunction. AIM: This study explores the mechanism 
      of extracellular vesicles (EVs) derived from human umbilical cord mesenchymal 
      stem cells (hucMSCs) affecting angiogenesis in MI via the microRNA 
      (miR)-423-5p/EFNA3 axis. MATERIAL AND METHODS: HucMSC-derived EVs (hucMSC-EVs) 
      were isolated, extracted, and identified. EVs and human umbilical vein 
      endothelial cells (HUVECs) were co-cultured. Migration capacity and angiogenesis 
      ability of HUVECs were measured, and VEGF levels in cell supernatants were tested 
      by ELISA. In-vivo rat MI models were established, and hucMSC-EVs were injected 
      into the MI rat heart-infarcted area. Cardiac function, capillary density, and 
      the degree of myocardial fibrosis were observed. RESULTS: HUVEC migration and 
      angiogenesis were promoted by hucMSC-EVs, and more significantly enhanced by 
      hucMSC-EVs containing miR-423-5p. Furthermore, miR-423-5p inhibited EFNA3 
      expression and EFNA3 overexpression reversed the promoting effects of EVs on 
      HUVEC migration and angiogenesis. miR-423-5p expression was elevated and EFNA3 
      expression was reduced in myocardial tissues of MI rats after EV treatment. Both 
      EVs and EVs containing miR-423-5p could improve cardiac function, reduce the area 
      of fibrosis, and promote angiogenesis, improving cardiac repair. CONCLUSIONS: EVs 
      promote in vivo angiogenesis in MI rats via the miR-423-5p/EFNA3 axis, thus 
      improving cardiac repair.
CI  - Copyright: (c) 2023 Termedia Sp. z o. o.
FAU - Gao, Tianlin
AU  - Gao T
AD  - Department of Cardiovascular Medicine, Ankang Central Hospital, Ankang City, 
      China.
FAU - Fan, Heng
AU  - Fan H
AD  - Department of Cardiovascular Medicine, Ankang Central Hospital, Ankang City, 
      China.
FAU - Wang, Jiawen
AU  - Wang J
AD  - Department of Cardiovascular Medicine, Ankang Central Hospital, Ankang City, 
      China.
FAU - Wang, Rui
AU  - Wang R
AD  - Department of Cardiovascular Medicine, Ankang Central Hospital, Ankang City, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20230202
PL  - Poland
TA  - Postepy Kardiol Interwencyjnej
JT  - Postepy w kardiologii interwencyjnej = Advances in interventional cardiology
JID - 101272671
PMC - PMC10031659
OTO - NOTNLM
OT  - EFNA3
OT  - angiogenesis
OT  - extracellular vesicles
OT  - human umbilical cord mesenchymal stem cells
OT  - microRNA-423-5p
OT  - myocardial infarction
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/28 06:00
MHDA- 2023/03/28 06:01
PMCR- 2022/12/01
CRDT- 2023/03/27 03:17
PHST- 2022/10/22 00:00 [received]
PHST- 2022/12/09 00:00 [accepted]
PHST- 2023/03/27 03:17 [entrez]
PHST- 2023/03/28 06:00 [pubmed]
PHST- 2023/03/28 06:01 [medline]
PHST- 2022/12/01 00:00 [pmc-release]
AID - 50088 [pii]
AID - 10.5114/aic.2023.124797 [doi]
PST - ppublish
SO  - Postepy Kardiol Interwencyjnej. 2022 Dec;18(4):373-391. doi: 
      10.5114/aic.2023.124797. Epub 2023 Feb 2.