PMID- 36968207
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230328
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 11
DP  - 2023
TI  - Retinal fluid is associated with cytokines of aqueous humor in age-related 
      macular degeneration using automatic 3-dimensional quantification.
PG  - 1157497
LID - 10.3389/fcell.2023.1157497 [doi]
LID - 1157497
AB  - Background: To explain the biological role of cytokines in the eye and the 
      possible role of cytokines in the pathogenesis of neovascular age-related macular 
      degeneration (nAMD) by comparing the correlation between cytokine of aqueous 
      humor concentration and optical coherence tomography (OCT) retinal fluid. 
      Methods: Spectral-domain OCT (SD-OCT) images and aqueous humor samples were 
      collected from 20 nAMD patient's three clinical visits. Retinal fluid volume in 
      OCT was automatically quantified using deep learning--Deeplabv3+. Eighteen 
      cytokines were detected in aqueous humor using the Luminex technology. OCT fluid 
      volume measurements were correlated with changes in aqueous humor cytokine levels 
      using Pearson's correlation coefficient (PCC). Results: The patients with 
      intraretinal fluid (IRF) showed significantly lower levels of cytokines, such as 
      C-X-C motif chemokine ligand 2 (CXCL2) (p = 0.03) and CXCL11 (p = 0.009), 
      compared with the patients without IRF. And the IRF volume was negatively 
      correlated with CXCL2 (r = -0.407, p = 0.048) and CXCL11 (r = -0.410, p = 0.046) 
      concentration in the patients with IRF. Meanwhile, the subretinal fluid (SRF) 
      volume was positively correlated with vascular endothelial growth factor (VEGF) 
      concentration (r = 0.299, p = 0.027) and negatively correlated with interleukin 
      (IL)-36beta concentration (r = -0.295, p = 0.029) in the patients with SRF. 
      Conclusion: Decreased level of VEGF was associated with decreased OCT-based 
      retinal fluid volume in nAMD patients, while increased levels of CXCL2, CXCL11, 
      and IL-36beta were associated with decreased OCT-based retinal fluid volume in nAMD 
      patients, which may suggest a role for inflammatory cytokines in retinal 
      morphological changes and pathogenesis of nAMD patients.
CI  - Copyright (c) 2023 Song, Jin, Wang, Yang, Zhou, Chen and Ye.
FAU - Song, Siyuan
AU  - Song S
AD  - Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang 
      University, Hangzhou, China.
FAU - Jin, Kai
AU  - Jin K
AD  - Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang 
      University, Hangzhou, China.
FAU - Wang, Shuai
AU  - Wang S
AD  - School of Mechanical, Electrical and Information Engineering, Shandong 
      University, Weihai, China.
AD  - School of Cyberspace, Hangzhou Dianzi University, Hangzhou, China.
FAU - Yang, Ce
AU  - Yang C
AD  - School of Mechanical, Electrical and Information Engineering, Shandong 
      University, Weihai, China.
FAU - Zhou, Jingxin
AU  - Zhou J
AD  - Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang 
      University, Hangzhou, China.
FAU - Chen, Zhiqing
AU  - Chen Z
AD  - Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang 
      University, Hangzhou, China.
FAU - Ye, Juan
AU  - Ye J
AD  - Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang 
      University, Hangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20230308
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC10030496
OTO - NOTNLM
OT  - age-related macular degeneration
OT  - cytokine
OT  - deep learning
OT  - optical coherence tomography
OT  - quantitative analysis
OT  - retinal fluid
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/03/28 06:00
MHDA- 2023/03/28 06:01
PMCR- 2023/01/01
CRDT- 2023/03/27 03:21
PHST- 2023/02/02 00:00 [received]
PHST- 2023/02/27 00:00 [accepted]
PHST- 2023/03/27 03:21 [entrez]
PHST- 2023/03/28 06:00 [pubmed]
PHST- 2023/03/28 06:01 [medline]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1157497 [pii]
AID - 10.3389/fcell.2023.1157497 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2023 Mar 8;11:1157497. doi: 10.3389/fcell.2023.1157497. 
      eCollection 2023.