PMID- 36970736
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230328
IS  - 2475-0379 (Electronic)
IS  - 2475-0379 (Linking)
VI  - 7
IP  - 1
DP  - 2023 Jan
TI  - Discriminating young platelets on human leukocyte antigen-I expression highlights 
      their extremely high reactivity potential.
PG  - 100006
LID - 10.1016/j.rpth.2022.100006 [doi]
LID - 100006
AB  - BACKGROUND: The platelet population is heterogeneous, with different subsets that 
      differ on the basis of their function and reactivity. An intrinsic factor 
      participating in this difference of reactivity could be the platelet age. The 
      lack of relevant tools allowing a formal identification of young platelets 
      prevents so far to draw solid conclusions regarding platelet reactivity. We 
      recently reported that human leukocyte antigen-I (HLA-I) molecules are more 
      expressed on human young platelets. OBJECTIVES: The aim of this study was to 
      assess platelet reactivity according to their age based on HLA-I expression 
      level. METHODS: Platelet activation was assessed by flow cytometry (FC) for 
      different platelet subsets based on their HLA-I expression. These populations 
      were further cell sorted and their intrinsic properties were determined by FC and 
      electron microscopy (EM). Statistical analyses were performed with GraphPad Prism 
      5.02 software using two-way ANOVA followed by a Tukey post hoc test. RESULTS: 
      HLA-I expression level allowed the identification of 3 platelet subpopulations 
      regarding to their age (HLA low, dim, and high). HLA-I was reliable to guide 
      platelet cell sorting and highlighted the features of young platelets in the 
      HLA-I(high) population. In response to different soluble agonists, HLA-I(high) 
      platelets were the most reactive subset as shown by the level of P-selectin 
      secretion and fibrinogen binding assessed by flow cytometry. Moreover, the 
      highest capacity of HLA-I(high) platelets to simultaneously express annexin-V and 
      von Willebrand factor or activated alphaIIbbeta3 after coactivation with TRAP and CRP 
      indicated that the procoagulant feature of platelets was age-related. CONCLUSION: 
      The young HLA-I(high) population is the most reactive and prone to become 
      procoagulant. These results open up new perspectives to investigate deeply the 
      role of young and old platelets.
CI  - (c) 2022 The Author(s).
FAU - Angenieux, Catherine
AU  - Angenieux C
AD  - UMR_S1255, INSERM, Strasbourg, France.
AD  - Etablissement Francais du Sang-Grand Est, Strasbourg, France.
AD  - Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
AD  - Universite de Strasbourg, Strasbourg, France.
FAU - Couvidou, Adele
AU  - Couvidou A
AD  - UMR_S1255, INSERM, Strasbourg, France.
AD  - Etablissement Francais du Sang-Grand Est, Strasbourg, France.
AD  - Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
AD  - Universite de Strasbourg, Strasbourg, France.
FAU - Brouard, Nathalie
AU  - Brouard N
AD  - UMR_S1255, INSERM, Strasbourg, France.
AD  - Etablissement Francais du Sang-Grand Est, Strasbourg, France.
AD  - Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
AD  - Universite de Strasbourg, Strasbourg, France.
FAU - Eckly, Anita
AU  - Eckly A
AD  - UMR_S1255, INSERM, Strasbourg, France.
AD  - Etablissement Francais du Sang-Grand Est, Strasbourg, France.
AD  - Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
AD  - Universite de Strasbourg, Strasbourg, France.
FAU - Dupuis, Arnaud
AU  - Dupuis A
AD  - UMR_S1255, INSERM, Strasbourg, France.
AD  - Etablissement Francais du Sang-Grand Est, Strasbourg, France.
AD  - Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
AD  - Universite de Strasbourg, Strasbourg, France.
FAU - Mangin, Pierre H
AU  - Mangin PH
AD  - UMR_S1255, INSERM, Strasbourg, France.
AD  - Etablissement Francais du Sang-Grand Est, Strasbourg, France.
AD  - Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
AD  - Universite de Strasbourg, Strasbourg, France.
FAU - Maitre, Blandine
AU  - Maitre B
AD  - UMR_S1255, INSERM, Strasbourg, France.
AD  - Etablissement Francais du Sang-Grand Est, Strasbourg, France.
AD  - Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg, France.
AD  - Universite de Strasbourg, Strasbourg, France.
LA  - eng
PT  - Journal Article
DEP - 20230214
PL  - United States
TA  - Res Pract Thromb Haemost
JT  - Research and practice in thrombosis and haemostasis
JID - 101703775
PMC - PMC10031328
OTO - NOTNLM
OT  - COAT, COllagen And Thrombin activated
OT  - CRP, Collagen Related Peptide
OT  - Conv, Convulxin
OT  - FC, Flow Cytometry
OT  - HLA-I, Human Leukocyte Antigen-I
OT  - P ribo prot, Anti-ribosomal P protein
OT  - TO, Thiazole orange
OT  - TRAP, Thrombin receptor activating peptide
OT  - electron microscopy
OT  - fluorescence-activated cell sorting
OT  - human class I antigens
OT  - platelets
OT  - procoagulant
OT  - vWF, Von Willebrand factor
EDAT- 2023/03/28 06:00
MHDA- 2023/03/28 06:01
PMCR- 2023/02/14
CRDT- 2023/03/27 03:57
PHST- 2022/08/22 00:00 [received]
PHST- 2022/10/19 00:00 [revised]
PHST- 2022/11/14 00:00 [accepted]
PHST- 2023/03/27 03:57 [entrez]
PHST- 2023/03/28 06:00 [pubmed]
PHST- 2023/03/28 06:01 [medline]
PHST- 2023/02/14 00:00 [pmc-release]
AID - S2475-0379(22)01500-X [pii]
AID - 100006 [pii]
AID - 10.1016/j.rpth.2022.100006 [doi]
PST - epublish
SO  - Res Pract Thromb Haemost. 2023 Feb 14;7(1):100006. doi: 
      10.1016/j.rpth.2022.100006. eCollection 2023 Jan.