PMID- 36971014
OWN - NLM
STAT- MEDLINE
DCOM- 20231020
LR  - 20231020
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 597
IP  - 9
DP  - 2023 May
TI  - MyD88 and not TRIF knockout is sufficient to abolish LPS-induced inflammatory 
      responses in bone-derived macrophages.
PG  - 1225-1232
LID - 10.1002/1873-3468.14616 [doi]
AB  - Macrophages play an important role in the response to infection and/or repair of 
      injury in tissues. To examine the NF-kappaB pathway in response to an inflammatory 
      stimulus, we used wild-type bone-marrow-derived macrophages (BMDMs) or BMDMs with 
      knockout (KO) of myeloid differentiation primary response 88 (MyD88) and/or 
      Toll/interleukin-1 receptor domain-containing adapter-inducing interferon-beta 
      (TRIF) via CRISPR/Cas9. Following treatment of BMDMs with lipopolysaccharide 
      (LPS) to induce an inflammatory response, translational signalling of NF-kappaB was 
      quantified via immunoblot and cytokines were measured. Our findings reveal that 
      MyD88 KO, but not TRIF KO, decreased LPS-induced NF-kappaB signalling, and 10% 
      expression of basal MyD88 expression was sufficient to partially rescue the 
      abolished inflammatory cytokine secretion observed upon MyD88 KO.
CI  - (c) 2023 Federation of European Biochemical Societies.
FAU - Reynoso, Marinaliz
AU  - Reynoso M
AD  - U.S. Army Research Institute of Environmental Medicine, Natick, MA, USA.
FAU - Hobbs, Stuart
AU  - Hobbs S
AD  - U.S. Army Research Institute of Environmental Medicine, Natick, MA, USA.
FAU - Kolb, Alexander L
AU  - Kolb AL
AD  - U.S. Army Research Institute of Environmental Medicine, Natick, MA, USA.
FAU - Matheny, Ronald W Jr
AU  - Matheny RW Jr
AD  - Military Operational Medicine Research Program, Fort Detrick, MA, USA.
FAU - Roberts, Brandon M
AU  - Roberts BM
AUID- ORCID: 0000-0003-0395-6967
AD  - U.S. Army Research Institute of Environmental Medicine, Natick, MA, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230404
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Adaptor Proteins, Vesicular Transport/genetics/metabolism
MH  - *Lipopolysaccharides/toxicity/metabolism
MH  - Macrophages/metabolism
MH  - Myeloid Differentiation Factor 88/genetics/metabolism
MH  - *NF-kappa B/genetics/metabolism
MH  - Toll-Like Receptor 4/genetics/metabolism
MH  - Animals
MH  - Mice
OTO - NOTNLM
OT  - CRISPR/Cas9
OT  - TLR4
OT  - bonemarrow-derived macrophages
OT  - inflammation
OT  - interleukin-10
OT  - tumour necrosis factor
EDAT- 2023/03/28 06:00
MHDA- 2023/05/10 06:42
CRDT- 2023/03/27 05:14
PHST- 2023/02/24 00:00 [revised]
PHST- 2023/01/20 00:00 [received]
PHST- 2023/02/28 00:00 [accepted]
PHST- 2023/05/10 06:42 [medline]
PHST- 2023/03/28 06:00 [pubmed]
PHST- 2023/03/27 05:14 [entrez]
AID - 10.1002/1873-3468.14616 [doi]
PST - ppublish
SO  - FEBS Lett. 2023 May;597(9):1225-1232. doi: 10.1002/1873-3468.14616. Epub 2023 Apr 
      4.