PMID- 36971858
OWN - NLM
STAT- MEDLINE
DCOM- 20230329
LR  - 20230410
IS  - 1436-5073 (Electronic)
IS  - 0026-3672 (Linking)
VI  - 190
IP  - 4
DP  - 2023 Mar 27
TI  - Determination of trypsin using protamine mediated fluorescent enhancement of DNA 
      templated Au nanoclusters.
PG  - 158
LID - 10.1007/s00604-023-05754-7 [doi]
AB  - A fluorescent method is described for trypsin determination through the strong 
      electrostatic interactions between cationic polyelectrolytes and single-stranded 
      DNA (ssDNA) templated Au nanoclusters (AuNCs). The ssDNA-AuNCs display improved 
      fluorescence emission with excitation/emission maxima at 280/475 nm after being 
      incorporated with poly(diallyldimethylammonium chloride) (PDDA). Fluorescent 
      enhancement is mainly attributed to the electrostatic interactions occurring  
      between PDDA and ssDNA templates. This can make the conformation of the ssDNA 
      templates to change. Thus, it offers a better microenvironment for stabilizing 
      and protecting ssDNA-AuNCs, and results in fluorescence emission enhancement. By 
      using protamine as a model, the method is employed for the determination of 
      trypsin. The assay enables trypsin to be determined with good sensitivity and a 
      linear response ranging from 5 ng⋅mL(-1) to 60 ng⋅mL(-1) with a 1.5 ng⋅mL(-1) 
      limit of detection. It is also extended to determine  the trypsin contents in 
      human's serum samples with recoveries between 98.7% and 103.5% with relative 
      standard deviations (RSDs) between 3.5% and 4.8%. A novel fluorescent strategy 
      has been developed for of trypsin determination by using protamine mediated 
      fluorescent enhancement of DNA templated Au nanoclusters.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Wu, Ning-Ning
AU  - Wu NN
AD  - College of Chemistry and Chemical Engineering, Xinyang Key Laboratory of 
      Functional Nanomaterials for Bioanalysis, Institute for Conservation and 
      Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, 
      Xinyang, 464000, People's Republic of China.
FAU - Chen, Lin-Ge
AU  - Chen LG
AD  - College of Chemistry and Chemical Engineering, Xinyang Key Laboratory of 
      Functional Nanomaterials for Bioanalysis, Institute for Conservation and 
      Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, 
      Xinyang, 464000, People's Republic of China.
FAU - Xiao, Min-Zhi
AU  - Xiao MZ
AD  - School of Chemistry and Chemical Engineering, Changsha University of Science and 
      Technology, Changsha, 410114, People's Republic of China.
FAU - Yuan, Rong-Yao
AU  - Yuan RY
AD  - School of Chemistry and Chemical Engineering, Changsha University of Science and 
      Technology, Changsha, 410114, People's Republic of China.
FAU - Wang, Hai-Bo
AU  - Wang HB
AUID- ORCID: 0000-0002-8064-1400
AD  - College of Chemistry and Chemical Engineering, Xinyang Key Laboratory of 
      Functional Nanomaterials for Bioanalysis, Institute for Conservation and 
      Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, 
      Xinyang, 464000, People's Republic of China. wanghaibohn@163.com.
LA  - eng
GR  - U1704153/National Natural Science Foundation of China/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230327
PL  - Austria
TA  - Mikrochim Acta
JT  - Mikrochimica acta
JID - 7808782
RN  - EC 3.4.21.4 (Trypsin)
RN  - 0 (Protamines)
RN  - 7440-57-5 (Gold)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (DNA, Single-Stranded)
SB  - IM
MH  - Humans
MH  - *Metal Nanoparticles
MH  - Trypsin
MH  - Protamines
MH  - Gold
MH  - Fluorescent Dyes
MH  - DNA, Single-Stranded
OTO - NOTNLM
OT  - Cationic polyelectrolytes
OT  - Conformation changes
OT  - Electrostatic interactions
OT  - Fluorescent method
EDAT- 2023/03/28 06:00
MHDA- 2023/03/29 06:05
CRDT- 2023/03/27 11:16
PHST- 2023/01/04 00:00 [received]
PHST- 2023/03/16 00:00 [accepted]
PHST- 2023/03/29 06:05 [medline]
PHST- 2023/03/27 11:16 [entrez]
PHST- 2023/03/28 06:00 [pubmed]
AID - 10.1007/s00604-023-05754-7 [pii]
AID - 10.1007/s00604-023-05754-7 [doi]
PST - epublish
SO  - Mikrochim Acta. 2023 Mar 27;190(4):158. doi: 10.1007/s00604-023-05754-7.