PMID- 36972692
OWN - NLM
STAT- MEDLINE
DCOM- 20230505
LR  - 20240502
IS  - 1476-1645 (Electronic)
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 108
IP  - 5
DP  - 2023 May 3
TI  - Colonizing and Virulence Factors in Enterotoxigenic Escherichia coli from Peru.
PG  - 948-953
LID - 10.4269/ajtmh.22-0677 [doi]
AB  - Enterotoxigenic Escherichia coli (ETEC) ranks among the most relevant 
      diarrheagenic pathogens. Efforts to design vaccines to fight ETEC have been 
      focused on colonizing factors (CFs) and atypical virulence factors (AVF). An 
      effective vaccine must account for differences in the regional prevalence of 
      these CFs and AVFs to be truly effective in a given area. In the present study, 
      the presence of 16 CFs and 9 AVFs, as well as the heat-stable (ST) variants (STh 
      or STp), was established by polymerase chain reaction in 205 Peruvian ETEC 
      isolates (120 from diarrhea cases and 85 from healthy controls). Ninety-nine 
      (48.3%) isolates were heat-labile, 63 (30.7%) ST, and 43 (21.0%) presented both 
      toxins. Of ST isolates, 59 (28.8%) possessed STh, 30 (14.6%) STp, five (2.4%) 
      both STh and STp, and 12 (5.8%) were not amplified for any variant tested. The 
      presence of CFs was associated with diarrhea (P < 0.0001). The presence of eatA 
      as well as concomitant presence of CSI, CS3, and CS21 and of C5 and C6 was 
      statistically related to diarrhea cases. The present results suggests that, if 
      effective, a vaccine considering CS6, CS20, and CS21, together with EtpA, would 
      provide protection against 64.4% of the isolates analyzed, whereas the addition 
      of CS12 and EAST1 would lead to 83.9% coverage. Large studies are needed to 
      establish both the ideal candidates to be considered to develop a vaccine 
      effective in the area, and continuous surveillance is needed to detect 
      displacement of circulating isolates that may compromise future vaccines.
FAU - Rivera, Fulton P
AU  - Rivera FP
AD  - Laboratorio de Enfermedades Entericas, Nutricion y Resistencia Antimicrobiana, 
      Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana 
      Cayetano Heredia, Lima, Peru.
AD  - Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, Peru.
AD  - Facultad de Ciencias de la Salud, Universidad Cientifica del Sur, Lima, Peru.
FAU - Medina, Anicia
AU  - Medina A
AD  - Laboratorio de Enfermedades Entericas, Nutricion y Resistencia Antimicrobiana, 
      Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana 
      Cayetano Heredia, Lima, Peru.
FAU - Riveros, Maribel
AU  - Riveros M
AD  - Laboratorio de Enfermedades Entericas, Nutricion y Resistencia Antimicrobiana, 
      Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana 
      Cayetano Heredia, Lima, Peru.
AD  - Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, Peru.
AD  - Facultad de Ciencias Naturales y Matematica, Universidad Nacional Federico 
      Villarreal, Lima, Peru.
AD  - Laboratorio de Infectologia Pediatrica, Universidad Peruana Cayetano Heredia, 
      Lima, Peru.
FAU - Ochoa, Theresa J
AU  - Ochoa TJ
AD  - Laboratorio de Enfermedades Entericas, Nutricion y Resistencia Antimicrobiana, 
      Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana 
      Cayetano Heredia, Lima, Peru.
AD  - Laboratorio de Infectologia Pediatrica, Universidad Peruana Cayetano Heredia, 
      Lima, Peru.
AD  - University of Texas School of Public Health, Houston, Texas.
FAU - Pons, Maria J
AU  - Pons MJ
AD  - Grupo de Enfermedades Emergentes y Reemergentes, Universidad Cientifica del Sur, 
      Lima, Peru.
FAU - Ruiz, Joaquim
AU  - Ruiz J
AD  - Grupo de Enfermedades Emergentes y Reemergentes, Universidad Cientifica del Sur, 
      Lima, Peru.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20230327
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (Virulence Factors)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Enterotoxins)
RN  - 0 (EtpA protein, E coli)
RN  - 0 (Membrane Glycoproteins)
SB  - IM
MH  - Humans
MH  - *Escherichia coli Infections/epidemiology
MH  - *Enterotoxigenic Escherichia coli/genetics
MH  - Peru/epidemiology
MH  - Virulence Factors/genetics
MH  - Diarrhea/epidemiology
MH  - *Escherichia coli Proteins/genetics
MH  - Enterotoxins
MH  - Membrane Glycoproteins
PMC - PMC10160877
EDAT- 2023/03/28 06:00
MHDA- 2023/05/05 06:42
PMCR- 2024/05/01
CRDT- 2023/03/27 19:22
PHST- 2022/10/25 00:00 [received]
PHST- 2023/01/12 00:00 [accepted]
PHST- 2023/05/05 06:42 [medline]
PHST- 2023/03/28 06:00 [pubmed]
PHST- 2023/03/27 19:22 [entrez]
PHST- 2024/05/01 00:00 [pmc-release]
AID - tpmd220677 [pii]
AID - 10.4269/ajtmh.22-0677 [doi]
PST - epublish
SO  - Am J Trop Med Hyg. 2023 Mar 27;108(5):948-953. doi: 10.4269/ajtmh.22-0677. Print 
      2023 May 3.