PMID- 36973836
OWN - NLM
STAT- MEDLINE
DCOM- 20230329
LR  - 20230329
IS  - 1006-7248 (Print)
IS  - 1006-7248 (Linking)
VI  - 32
IP  - 1
DP  - 2023 Feb
TI  - [An improved paraffin embedding method for small core needle biopsy: technical 
      introduction and evaluation].
PG  - 6-11
AB  - PURPOSE: Modified agar pre-embedded paraffin embedding method was proposed to 
      evaluate the effects on tissue integrity, histological morphology, protein and 
      DNA detection in small specimens of core needle biopsies. METHODS: The core 
      needle biopsy specimens of 10 patients with oral mucosal squamous cell carcinoma 
      were subjected to modified agar pre-embedded paraffin embedding using molded 
      embedding molds and conventional paraffin embedding respectively, the dehydration 
      time of the former was 3.5 h and that of the latter was 12 h. After tissue 
      treatment, H-E staining, histological morphology, immunohistochemistry (IHC), and 
      DNA fluorescence in situ hybridization (FISH) were performed, respectively. The 
      results were compared and analyzed using GraphPad Prism 9 software package. 
      RESULTS: The modified agar pre-embedding method was less difficult to perform 
      than the agar pre-embedding method, and easier to be promoted. Compared with 
      conventional paraffin embedding method, the tissue dehydration time was 
      significantly reduced(P＜0.001), and the results of microscopic histological 
      morphology and subsequent IHC and FISH assays were reliable. CONCLUSIONS: The 
      modified agar pre-embedded paraffin embedding method meets the requirements of 
      clinical pathological diagnosis for tissue processing, and is worthy of clinical 
      application for core needle biopsy specimens.
FAU - Chen, Wan-Ling
AU  - Chen WL
AD  - Department of Oral Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao 
      Tong University School of Medicine; College of Stomatology, Shanghai Jiao Tong 
      University; National Center for Stomatology; National Clinical Research Center 
      for Oral Diseases; Shanghai Key Laboratory of Stomatology; Shanghai Research 
      Institute of Stomatology. Shanghai 200011, China. E-mail: cwl200940@live.cn.
FAU - Shi, Chao-Ji
AU  - Shi CJ
FAU - Wang, Min
AU  - Wang M
FAU - Gu, Ting
AU  - Gu T
FAU - Tian, Zhen
AU  - Tian Z
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Shanghai Kou Qiang Yi Xue
JT  - Shanghai kou qiang yi xue = Shanghai journal of stomatology
JID - 101090220
RN  - 9002-18-0 (Agar)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Humans
MH  - Paraffin Embedding
MH  - Biopsy, Large-Core Needle
MH  - In Situ Hybridization, Fluorescence
MH  - Agar
MH  - *Dehydration
MH  - DNA
MH  - *Head and Neck Neoplasms
MH  - Biopsy
EDAT- 2023/03/28 06:00
MHDA- 2023/03/29 06:05
CRDT- 2023/03/27 23:43
PHST- 2023/03/29 06:05 [medline]
PHST- 2023/03/27 23:43 [entrez]
PHST- 2023/03/28 06:00 [pubmed]
PST - ppublish
SO  - Shanghai Kou Qiang Yi Xue. 2023 Feb;32(1):6-11.