PMID- 36975521
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230330
IS  - 1467-3045 (Electronic)
IS  - 1467-3037 (Print)
IS  - 1467-3037 (Linking)
VI  - 45
IP  - 3
DP  - 2023 Mar 12
TI  - Transcriptomic Establishment of Pig Macrophage Polarization Signatures.
PG  - 2338-2350
LID - 10.3390/cimb45030151 [doi]
AB  - Macrophages are the foremost controllers of innate and acquired immunity, playing 
      important roles in tissue homeostasis, vasculogenesis, and congenital metabolism. 
      In vitro macrophages are crucial models for understanding the regulatory 
      mechanism of immune responses and the diagnosis or treatment of a variety of 
      diseases. Pigs are the most important agricultural animals and valuable animal 
      models for preclinical studies, but there is no unified method for porcine 
      macrophage isolation and differentiation at present; no systematic study has 
      compared porcine macrophages obtained by different methods. In the current study, 
      we obtained two M1 macrophages (M1_IFNgamma + LPS, and M1_GM-CSF) and two M2 
      macrophages (M2_IL4 + IL10, and M2_M-CSF), and compared the transcriptomic 
      profiles between and within macrophage phenotypes. We observed the 
      transcriptional differences either between or within phenotypes. Porcine M1 and 
      M2 macrophages have consistent gene signatures with human and mouse macrophage 
      phenotypes, respectively. Moreover, we performed GSEA analysis to attribute the 
      prognostic value of our macrophage signatures in discriminating various pathogen 
      infections. Our study provided a framework to guide the interrogation of 
      macrophage phenotypes in the context of health and disease. The approach 
      described here could be used to propose new biomarkers for diagnosis in diverse 
      clinical settings including porcine reproductive and respiratory syndrome virus 
      (PRRSV), African swine fever virus (ASFV), Toxoplasma gondii (T. gondii), porcine 
      circovirus type 2 (PCV2), Haemophilus parasuis serovar 4 (HPS4), Mycoplasma 
      hyopneumoniae (Mhp), Streptococcus suis serotype 2 (SS2), and LPS from Salmonella 
      enterica serotype minnesota Re 595.
FAU - Li, Jing
AU  - Li J
AUID- ORCID: 0000-0001-5921-8096
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
FAU - Yuan, Teng
AU  - Yuan T
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
FAU - Zhang, Anjing
AU  - Zhang A
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
FAU - Yang, Peidong
AU  - Yang P
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
FAU - He, Li
AU  - He L
AUID- ORCID: 0000-0001-7854-0304
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
FAU - Long, Keren
AU  - Long K
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
FAU - Tang, Chuang
AU  - Tang C
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
FAU - Chen, Li
AU  - Chen L
AD  - Chongqing Academy of Animal Sciences, Chongqing 402460, China.
FAU - Li, Mingzhou
AU  - Li M
AUID- ORCID: 0000-0001-8681-7684
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
FAU - Lu, Lu
AU  - Lu L
AUID- ORCID: 0000-0002-0960-1529
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu 611130, China.
LA  - eng
GR  - 2021YFD1300800 and 2020YFA0509500/the National Key R & D Program of China/
GR  - 2021YFS0008 and 2022YFQ0022/the Science Foundation of the Sichuan Province/
GR  - 2021M692329/the China Postdoctoral Science Foundation/
PT  - Journal Article
DEP - 20230312
PL  - Switzerland
TA  - Curr Issues Mol Biol
JT  - Current issues in molecular biology
JID - 100931761
PMC - PMC10047103
OTO - NOTNLM
OT  - GSEA
OT  - differentiation
OT  - macrophage
OT  - porcine
OT  - transcriptome
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/29 06:00
MHDA- 2023/03/29 06:01
PMCR- 2023/03/12
CRDT- 2023/03/28 09:14
PHST- 2023/02/10 00:00 [received]
PHST- 2023/03/04 00:00 [revised]
PHST- 2023/03/07 00:00 [accepted]
PHST- 2023/03/29 06:01 [medline]
PHST- 2023/03/28 09:14 [entrez]
PHST- 2023/03/29 06:00 [pubmed]
PHST- 2023/03/12 00:00 [pmc-release]
AID - cimb45030151 [pii]
AID - cimb-45-00151 [pii]
AID - 10.3390/cimb45030151 [doi]
PST - epublish
SO  - Curr Issues Mol Biol. 2023 Mar 12;45(3):2338-2350. doi: 10.3390/cimb45030151.