PMID- 36976396
OWN - NLM
STAT- MEDLINE
DCOM- 20230330
LR  - 20230330
IS  - 1432-2048 (Electronic)
IS  - 0032-0935 (Linking)
VI  - 257
IP  - 5
DP  - 2023 Mar 28
TI  - The Arabidopsis Phytoglobin 2 mediates phytochrome B (phyB) light signaling 
      responses during somatic embryogenesis.
PG  - 88
LID - 10.1007/s00425-023-04121-3 [doi]
AB  - During the light induction of somatic embryogenesis, phyB-Pfr suppresses 
      Phytoglobin 2, known to elevate nitric oxide (NO). NO depresses Phytochrome 
      Interacting Factor 4 (PIF4) relieving its inhibition on embryogenesis through 
      auxin. An obligatory step of many in vitro embryogenic systems is the 
      somatic-embryogenic transition culminating with the formation of the embryogenic 
      tissue. In Arabidopsis, this transition requires light and is facilitated by high 
      levels of nitric oxide (NO) generated by either suppression of the NO scavenger 
      Phytoglobin 2 (Pgb2), or its removal from the nucleus. Using a previously 
      characterized induction system regulating the cellular localization of Pgb2, we 
      demonstrated the interplay between phytochrome B (phyB) and Pgb2 during the 
      formation of embryogenic tissue. The deactivation of phyB in the dark coincides 
      with the induction of Pgb2 known to reduce the level of NO; consequently, 
      embryogenesis is inhibited. Under light conditions, the active form of phyB 
      depresses the levels of Pgb2 transcripts, thus expecting an increase in cellular 
      NO. Induction of Pgb2 increases Phytochrome Interacting Factor 4 (PIF4) 
      suggesting that high levels of NO repress PIF4. The PIF4 inhibition is sufficient 
      to induce several auxin biosynthetic (CYP79B2, AMI1, and YUCCA 1, 2, and 6) and 
      response (ARF5, 8, and 16) genes, conducive to the formation of the embryonic 
      tissue and production of somatic embryos. Auxin responses mediated by ARF10 and 
      17 appear to be regulated by Pgb2, possibly through NO, in a PIF4-independent 
      fashion. Overall, this work provides a new and preliminary model integrating Pgb2 
      (and NO) with phyB in the light regulation of in vitro embryogenesis.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Mira, Mohammed M
AU  - Mira MM
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, 
      Canada.
AD  - Department of Botany, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
FAU - Day, Sam
AU  - Day S
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, 
      Canada.
FAU - Ibrahim, Shimaa
AU  - Ibrahim S
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, 
      Canada.
FAU - Hill, Robert D
AU  - Hill RD
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, 
      Canada.
FAU - Stasolla, Claudio
AU  - Stasolla C
AD  - Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, 
      Canada. stasolla@ms.umanitoba.ca.
LA  - eng
GR  - 13245/NSERC/
PT  - Journal Article
DEP - 20230328
PL  - Germany
TA  - Planta
JT  - Planta
JID - 1250576
RN  - 136250-22-1 (Phytochrome B)
RN  - 0 (Arabidopsis Proteins)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 11121-56-5 (Phytochrome)
RN  - 0 (Indoleacetic Acids)
RN  - 0 (PHYB protein, Arabidopsis)
SB  - IM
MH  - *Arabidopsis/physiology
MH  - Phytochrome B/genetics
MH  - *Arabidopsis Proteins/genetics/metabolism
MH  - Nitric Oxide
MH  - *Phytochrome/genetics
MH  - Indoleacetic Acids
MH  - Light
MH  - Gene Expression Regulation, Plant
OTO - NOTNLM
OT  - Auxin
OT  - Light signaling
OT  - Nitric oxide
OT  - Phytoglobin
OT  - Somatic embryogenesis
EDAT- 2023/03/29 06:00
MHDA- 2023/03/30 06:11
CRDT- 2023/03/28 11:17
PHST- 2023/02/07 00:00 [received]
PHST- 2023/03/13 00:00 [accepted]
PHST- 2023/03/30 06:11 [medline]
PHST- 2023/03/28 11:17 [entrez]
PHST- 2023/03/29 06:00 [pubmed]
AID - 10.1007/s00425-023-04121-3 [pii]
AID - 10.1007/s00425-023-04121-3 [doi]
PST - epublish
SO  - Planta. 2023 Mar 28;257(5):88. doi: 10.1007/s00425-023-04121-3.