PMID- 36977141 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230331 IS - 2414-6366 (Electronic) IS - 2414-6366 (Linking) VI - 8 IP - 3 DP - 2023 Feb 25 TI - Discrepancy in Response of Mouse Dendritic Cells against BCG: Weak Immune Effects of Plasmacytoid Dendritic Cells Compared to Classical Dendritic Cells despite the Uptake of Bacilli. LID - 10.3390/tropicalmed8030140 [doi] LID - 140 AB - Tuberculosis (TB), a zoonosis characterized by chronic respiratory infections, is mainly caused by Mycobacterium tuberculosis and is associated with one of the heaviest disease burdens in the world. Dendritic cells (DCs) play a key role and act as a bridge between innate and adaptive immune responses against TB. DCs are divided into distinct subsets. Currently, the response of DCs to mycobacterial infections is poorly understood. Herein, we aimed to evaluate the responses of splenic conventional DCs (cDC) and plasmacytoid DCs (pDC), subsets to Bacillus Calmette-Guerin (BCG) infection in mice. Splenic pDC had a significantly higher infection rate and intracellular bacterial count than cDC and the CD8(+) and CD8(-) cDC subsets after BCG infection. However, the expression levels of CD40, CD80, CD86, and MHC-II molecules were significantly upregulated in splenic cDC and the CD8 cDC subsets compared to pDC during BCG infection. Splenic cDC had a higher expression of IFN-gamma and IL-12p70 than pDC, whereas pDC had higher levels of TNF-alpha and MCP-1 than cDC in mice infected with BCG. At early stages of immunization with BCG containing the Ag85A protein, splenic cDC and pDC could present the Ag85A peptide to a specific T hybridoma; however, cDC had a stronger antigen presenting activity than pDC. In summary, splenic cDC and pDC extensively participate in mouse immune responses against BCG infection in vivo. Although pDC had a higher BCG uptake, cDC induced stronger immunological effects, including activation and maturation, cytokine production, and antigen presentation. FAU - Meng, Chuang AU - Meng C AUID- ORCID: 0000-0001-6436-1321 AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. FAU - Liu, Jun AU - Liu J AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. FAU - Kang, Xilong AU - Kang X AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. FAU - Xu, Zhengzhong AU - Xu Z AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. FAU - Xu, Shuangyuan AU - Xu S AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. FAU - Li, Xin AU - Li X AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. FAU - Pan, Zhiming AU - Pan Z AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. FAU - Chen, Xiang AU - Chen X AUID- ORCID: 0000-0002-9736-8180 AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. FAU - Jiao, Xinan AU - Jiao X AUID- ORCID: 0000-0002-2214-3358 AD - Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China. AD - Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. LA - eng GR - 2021YFD1800403/the National Key Research and Development Program of China/ GR - 32002295/the National Natural Science Foundation of China/ GR - CX(21)1004/the Jiangsu Agriculture Science and Technology Innovation Fund/ PT - Journal Article DEP - 20230225 PL - Switzerland TA - Trop Med Infect Dis JT - Tropical medicine and infectious disease JID - 101709042 PMC - PMC10057906 OTO - NOTNLM OT - BCG OT - classic dendritic cells OT - immune response OT - in vivo OT - plasmacytoid dendritic cells COIS- The authors declare no conflict of interest. EDAT- 2023/03/29 06:00 MHDA- 2023/03/29 06:01 PMCR- 2023/02/25 CRDT- 2023/03/28 15:22 PHST- 2022/12/19 00:00 [received] PHST- 2023/01/25 00:00 [revised] PHST- 2023/02/24 00:00 [accepted] PHST- 2023/03/29 06:01 [medline] PHST- 2023/03/28 15:22 [entrez] PHST- 2023/03/29 06:00 [pubmed] PHST- 2023/02/25 00:00 [pmc-release] AID - tropicalmed8030140 [pii] AID - tropicalmed-08-00140 [pii] AID - 10.3390/tropicalmed8030140 [doi] PST - epublish SO - Trop Med Infect Dis. 2023 Feb 25;8(3):140. doi: 10.3390/tropicalmed8030140.