PMID- 36977230 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20230331 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 10 IP - 3 DP - 2023 Mar 3 TI - Porcine Reproductive and Respiratory Syndrome Virus Engineered by Serine Substitution on the 44th Amino Acid of GP5 Resulted in a Potential Vaccine Candidate with the Ability to Produce High Levels of Neutralizing Antibody. LID - 10.3390/vetsci10030191 [doi] LID - 191 AB - N-linked glycans covering GP5 neutralizing epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) have been proposed to act as a sheath blocking the production of neutralizing antibodies. Herein, we genetically engineered PRRSV with serine (S) substitution on the 44th asparagine (N) on the GP5 ectodomain of PRRSV-2 lineage-1. To evaluate the recombinant PRRSV, in vivo experiments were performed in piglets. The recombinant virus group showed no viremia until 42 days post-inoculation (dpi), and the rectal temperature and average daily weight gain were in the normal range at the same time point as the negative control group. On the 42 dpi, both groups were challenged with the wild-type virus. The recombinant PRRSV group showed lower rectal temperature, viremia, and the lung lesions than that of the negative control group for 19 days post-challenge (dpc). Additionally, the recombinant virus induced 4.50 +/- 3.00 (log(2)) and 8.25 +/- 0.96 (log(2)) of neutralizing antibody before and after challenge, respectively. Taken together, this study confirmed that N44S substitution can create an infectious PRRSV that strongly induces neutralizing antibodies. In addition, the vCSL1-GP5-N44S mutant that we produced was confirmed to have potential as a vaccine candidate, showing good safety and protective effects in pigs. FAU - Choi, Jong-Chul AU - Choi JC AUID- ORCID: 0000-0003-3099-6040 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Kim, Min-Sik AU - Kim MS AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Choi, Hwi-Yeon AU - Choi HY AUID- ORCID: 0000-0001-7773-0982 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Kang, Yeong-Lim AU - Kang YL AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Choi, In-Yeong AU - Choi IY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Jung, Sung-Won AU - Jung SW AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Jeong, Ji-Yun AU - Jeong JY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. AD - Careside Co., Ltd., Woolim Lions Valley A-B210, #146-8, Sangdaewon-dong, Jungwon-gu, Seongnam 13209, Gyeonggi-do, Republic of Korea. FAU - Kim, Min-Chul AU - Kim MC AD - Careside Co., Ltd., Woolim Lions Valley A-B210, #146-8, Sangdaewon-dong, Jungwon-gu, Seongnam 13209, Gyeonggi-do, Republic of Korea. FAU - Cho, Andrew Y AU - Cho AY AUID- ORCID: 0000-0003-2918-2349 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Lee, Ji-Ho AU - Lee JH AD - Southeast Poultry Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, U.S. National Poultry Research Center, Athens, GA 30605, USA. FAU - Lee, Dong-Hun AU - Lee DH AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Lee, Sang-Won AU - Lee SW AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Park, Seung-Yong AU - Park SY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Song, Chang-Seon AU - Song CS AUID- ORCID: 0000-0002-4158-6402 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Choi, In-Soo AU - Choi IS AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. FAU - Lee, Joong-Bok AU - Lee JB AUID- ORCID: 0000-0003-1074-8162 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Republic of Korea. LA - eng GR - 320005-04/Ministry of Agriculture, Food and Rural Affairs/ GR - 0000000/Careside/ PT - Journal Article DEP - 20230303 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC10055445 OTO - NOTNLM OT - GP5 OT - glycosylation OT - neutralizing antibody OT - porcine reproductive and respiratory syndrome virus OT - vaccine COIS- J.-Y.J. and M.-C.K. are employees of Careside (Seongnam, Korea). The rest of the authors declare that they have no relevant conflict of interest. EDAT- 2023/03/29 06:00 MHDA- 2023/03/29 06:01 PMCR- 2023/03/03 CRDT- 2023/03/28 15:53 PHST- 2023/01/24 00:00 [received] PHST- 2023/03/01 00:00 [revised] PHST- 2023/03/02 00:00 [accepted] PHST- 2023/03/29 06:01 [medline] PHST- 2023/03/28 15:53 [entrez] PHST- 2023/03/29 06:00 [pubmed] PHST- 2023/03/03 00:00 [pmc-release] AID - vetsci10030191 [pii] AID - vetsci-10-00191 [pii] AID - 10.3390/vetsci10030191 [doi] PST - epublish SO - Vet Sci. 2023 Mar 3;10(3):191. doi: 10.3390/vetsci10030191.