PMID- 36978309
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230331
IS  - 2079-6382 (Print)
IS  - 2079-6382 (Electronic)
IS  - 2079-6382 (Linking)
VI  - 12
IP  - 3
DP  - 2023 Feb 23
TI  - Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans 
      and Staphylococcus aureus.
LID - 10.3390/antibiotics12030441 [doi]
LID - 441
AB  - The natural bioactive molecule farnesol (FAR) is widely studied mainly for its 
      antibiofilm and antimicrobial properties. In addition, it increases the 
      effectiveness of some antimicrobial substances, which makes it interesting for 
      the development of combined therapy. In the present work, the effect of FAR 
      either alone or in combination with oxacillin (OXA) on mixed biofilms formed by 
      clinically relevant pathogens, Candida albicans and Staphylococcus aureus, was 
      studied. S. aureus isolates used for biofilm formation originated from blood 
      cultures and central venous catheters (CVC) were characterized in terms of 
      antimicrobial resistance. The minimal biofilm inhibitory concentration (MBIC(50)) 
      for FAR of 48 h mixed biofilms formed by the C. albicans and 
      methicillin-sensitive S. aureus (MSSA) was determined to be 125 muM, and for the 
      mixed biofilms with methicillin-resistant S. aureus (MRSA) was determined to be 
      250 muM. Treatment of mixed biofilms with OXA (2 mg/mL) showed </=4% inhibition; 
      however, the combination of OXA (2 mg/mL) and FAR (300 muM) resulted in 80% 
      inhibition of biofilms. In addition, planktonic cells of S. aureus exhibited an 
      increased susceptibility to OXA, cefoxitin and kanamycin in the presence of FAR 
      (150 and 300 muM). Scanning electron microscopy (SEM) micrographs confirmed patchy 
      biofilm and lack of candidal hyphae in the samples treated with FAR and FAR/OXA 
      in comparison to control and mixed biofilms treated only with OXA. Intriguingly, 
      in a pilot experiment using fluorescence in situ hybridization (FISH), 
      considerable differences in activity (as indicated by ribosome content) of 
      staphylococcal cells were detected. While the activity rate of the staphylococci 
      in mixed biofilms treated with FAR was high, no FISH-positive signal for 
      staphylococcal cells was found in the biofilm treated with FAR/OXA.
FAU - Gaalova-Radochova, Barbora
AU  - Gaalova-Radochova B
AUID- ORCID: 0000-0003-0448-4658
AD  - Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius 
      University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia.
FAU - Kendra, Samuel
AU  - Kendra S
AD  - Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius 
      University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia.
FAU - Jordao, Luisa
AU  - Jordao L
AUID- ORCID: 0000-0002-5608-021X
AD  - Department of Environmental Health, Research and Development Unit, National 
      Institute of Health Dr. Ricardo Jorge (INSA), Av. Padre Cruz, 1649-016 Lisboa, 
      Portugal.
FAU - Kursawe, Laura
AU  - Kursawe L
AD  - Biofilmcenter, Institute of Microbiology, Infectious Diseases and Immunology, 
      Charite-Universitatsmedizin Berlin, Hindenburgdamm 30, 12203 Berlin, Germany.
AD  - MoKi Analytics GmbH, Charite-Universitatsmedizin Berlin, Hindenburdamm 30, 12203 
      Berlin, Germany.
FAU - Kikhney, Judith
AU  - Kikhney J
AD  - Biofilmcenter, Institute of Microbiology, Infectious Diseases and Immunology, 
      Charite-Universitatsmedizin Berlin, Hindenburgdamm 30, 12203 Berlin, Germany.
AD  - MoKi Analytics GmbH, Charite-Universitatsmedizin Berlin, Hindenburdamm 30, 12203 
      Berlin, Germany.
FAU - Moter, Annette
AU  - Moter A
AD  - Biofilmcenter, Institute of Microbiology, Infectious Diseases and Immunology, 
      Charite-Universitatsmedizin Berlin, Hindenburgdamm 30, 12203 Berlin, Germany.
AD  - MoKi Analytics GmbH, Charite-Universitatsmedizin Berlin, Hindenburdamm 30, 12203 
      Berlin, Germany.
AD  - Moter Diagnostics, Marienplatz 9, 12207 Berlin, Germany.
FAU - Bujdakova, Helena
AU  - Bujdakova H
AUID- ORCID: 0000-0002-9219-0803
AD  - Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius 
      University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia.
LA  - eng
GR  - SK-PT-18-0006/Slovak Research and Development Agency/
GR  - APVV-21-0302/Slovak Research and Development Agency/
GR  - APVV-18-0075/Slovak Research and Development Agency/
GR  - 952398-CEMBO/H2020-WIDESPREAD-05-2020-Twinning/
PT  - Journal Article
DEP - 20230223
PL  - Switzerland
TA  - Antibiotics (Basel)
JT  - Antibiotics (Basel, Switzerland)
JID - 101637404
PMC - PMC10044556
OTO - NOTNLM
OT  - biofilms of C. albicans-MSSA/MRSA
OT  - cell viability
OT  - enhancing effect
OT  - farnesol
OT  - methicillin-resistant/sensitive S. aureus
OT  - microscopy
OT  - resistance
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:01
PMCR- 2023/02/23
CRDT- 2023/03/29 01:13
PHST- 2023/02/01 00:00 [received]
PHST- 2023/02/19 00:00 [revised]
PHST- 2023/02/21 00:00 [accepted]
PHST- 2023/03/30 06:01 [medline]
PHST- 2023/03/29 01:13 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/02/23 00:00 [pmc-release]
AID - antibiotics12030441 [pii]
AID - antibiotics-12-00441 [pii]
AID - 10.3390/antibiotics12030441 [doi]
PST - epublish
SO  - Antibiotics (Basel). 2023 Feb 23;12(3):441. doi: 10.3390/antibiotics12030441.