PMID- 36978702
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230331
IS  - 2306-5354 (Print)
IS  - 2306-5354 (Electronic)
IS  - 2306-5354 (Linking)
VI  - 10
IP  - 3
DP  - 2023 Mar 1
TI  - Investigating the Extracellular-Electron-Transfer Mechanisms and Kinetics of 
      Shewanella decolorationis NTOU1 Reducing Graphene Oxide via Lactate Metabolism.
LID - 10.3390/bioengineering10030311 [doi]
LID - 311
AB  - Microbial graphene oxide reduction is a developing method that serves to reduce 
      both production costs and environmental impact in the synthesis of graphene. This 
      study demonstrates microbial graphene oxide reduction using Shewanella 
      decolorationis NTOU1 under neutral and mild conditions (pH = 7, 35  degrees C, and 1 
      atm). Graphene oxide (GO) prepared via the modified Hummers' method is used as 
      the sole solid electron acceptor, and the characteristics of reduced GO (rGO) are 
      investigated. According to electron microscopic images, the surface structure of 
      GO was clearly changed from smooth to wrinkled after reduction, and whole cells 
      were observed to be wrapped by GO/rGO films. Distinctive appendages on the cells, 
      similar to nanowires or flagella, were also observed. With regard to 
      chemical-bonding changes, after a 24-h reaction of 1 mg mL(-1), GO was reduced to 
      rGO, the C/O increased from 1.4 to 3.0, and the oxygen-containing functional 
      groups of rGO were significantly reduced. During the GO reduction process, the 
      number of S. decolorationis NTOU1 cells decreased from 1.65 x 10(8) to 1.03 x 
      10(6) CFU mL(-1), indicating the bactericide effects of GO/rGO. In experiments 
      adding consistent concentrations of initial bacteria and lactate, it was shown 
      that with the increase of GO additions (0.5-5.0 mg mL(-1)), the first-order 
      reaction rate constants (k) of lactate metabolism and acetate production 
      increased accordingly; in experiments adding consistent concentrations of initial 
      bacteria and GO but different lactate levels (1 to 10 mM), the k values of 
      lactate metabolism did not change significantly. The test results of adding 
      different electron transfer mediators showed that riboflavin and potassium 
      ferricyanide were able to boost GO reduction, whereas 
      2,6-dimethoxy-1,4-benzoquinone and 2,6-dimethyl benzoquinone completely 
      eliminated bacterial activity.
FAU - Liou, Yu-Xuan
AU  - Liou YX
AD  - Department of Environmental Science and Engineering, Tunghai University, Taichung 
      40704, Taiwan.
FAU - Li, Shiue-Lin
AU  - Li SL
AUID- ORCID: 0000-0002-3883-6087
AD  - Department of Environmental Science and Engineering, Tunghai University, Taichung 
      40704, Taiwan.
FAU - Hsieh, Kun-Yi
AU  - Hsieh KY
AD  - Department of Environmental Science and Engineering, Tunghai University, Taichung 
      40704, Taiwan.
FAU - Li, Sin-Jie
AU  - Li SJ
AD  - Department of Environmental Science and Engineering, Tunghai University, Taichung 
      40704, Taiwan.
FAU - Hu, Li-Jie
AU  - Hu LJ
AD  - Department of Environmental Science and Engineering, Tunghai University, Taichung 
      40704, Taiwan.
LA  - eng
GR  - MOST 110-2221-E-029-018/Ministry of Science and Technology, Taiwan/
PT  - Journal Article
DEP - 20230301
PL  - Switzerland
TA  - Bioengineering (Basel)
JT  - Bioengineering (Basel, Switzerland)
JID - 101676056
PMC - PMC10045794
OTO - NOTNLM
OT  - Shewanella decolorationis NTOU1
OT  - extracellular electron transfer
OT  - mediator addition
OT  - microbial graphene oxide reduction
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:01
PMCR- 2023/03/01
CRDT- 2023/03/29 01:15
PHST- 2023/01/31 00:00 [received]
PHST- 2023/02/24 00:00 [revised]
PHST- 2023/02/27 00:00 [accepted]
PHST- 2023/03/30 06:01 [medline]
PHST- 2023/03/29 01:15 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/01 00:00 [pmc-release]
AID - bioengineering10030311 [pii]
AID - bioengineering-10-00311 [pii]
AID - 10.3390/bioengineering10030311 [doi]
PST - epublish
SO  - Bioengineering (Basel). 2023 Mar 1;10(3):311. doi: 
      10.3390/bioengineering10030311.