PMID- 36978778
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230331
IS  - 2306-5354 (Print)
IS  - 2306-5354 (Electronic)
IS  - 2306-5354 (Linking)
VI  - 10
IP  - 3
DP  - 2023 Mar 21
TI  - Focused Low-Intensity Pulsed Ultrasound (FLIPUS) Mitigates Apoptosis of MLO-Y4 
      Osteocyte-like Cells.
LID - 10.3390/bioengineering10030387 [doi]
LID - 387
AB  - Long cytoplasmic processes of osteocytes orchestrate bone activity by integration 
      of biochemical and mechanical signals and regulate load-induced bone adaptation. 
      Low-Intensity Pulsed Ultrasound (LIPUS) is a clinically used technique for 
      fracture healing that delivers mechanical impulses to the damaged bone tissue in 
      a non-invasive and non-ionizing manner. The mechanism of action of LIPUS is still 
      controversially discussed in the scientific community. In this study, the effect 
      of focused LIPUS (FLIPUS) on the survival of starved MLO-Y4 osteocytes was 
      investigated in vitro. Osteocytes stimulated for 10 min with FLIPUS exhibited 
      extended dendrites, which formed frequent connections to neighboring cells and 
      spanned longer distances. The sonicated cells displayed thick actin bundles and 
      experienced increase in expression of connexin 43 (Cx43) proteins, especially on 
      their dendrites, and E11 glycoprotein, which is responsible for the elongation of 
      cellular cytoplasmic processes. After stimulation, expression of cell growth and 
      survival genes as well as genes related to cell-cell communication was augmented. 
      In addition, cell viability was improved after the sonication, and a decrease in 
      ATP release in the medium was observed. In summary, FLIPUS mitigated apoptosis of 
      starved osteocytes, which is likely related to the formation of the extensive 
      dendritic network that ensured cell survival.
FAU - Puts, Regina
AU  - Puts R
AUID- ORCID: 0000-0002-2019-328X
AD  - Center for Biomedicine, Charite-Universitatsmedizin, 12203 Berlin, Germany.
AD  - Berlin Institute of Health (BIH) Center for Regenerative Therapies, 
      Charite-Universitatsmedizin, 13353 Berlin, Germany.
FAU - Khaffaf, Aseel
AU  - Khaffaf A
AD  - Center for Biomedicine, Charite-Universitatsmedizin, 12203 Berlin, Germany.
FAU - Shaka, Maria
AU  - Shaka M
AD  - Center for Biomedicine, Charite-Universitatsmedizin, 12203 Berlin, Germany.
FAU - Zhang, Hui
AU  - Zhang H
AD  - Center for Biomedicine, Charite-Universitatsmedizin, 12203 Berlin, Germany.
FAU - Raum, Kay
AU  - Raum K
AUID- ORCID: 0000-0003-0573-1622
AD  - Center for Biomedicine, Charite-Universitatsmedizin, 12203 Berlin, Germany.
LA  - eng
GR  - Raum 1380/10-1/Deutsche Forschungsgemeinschaft/
GR  - PU 701/1-1/Deutsche Forschungsgemeinschaft/
PT  - Journal Article
DEP - 20230321
PL  - Switzerland
TA  - Bioengineering (Basel)
JT  - Bioengineering (Basel, Switzerland)
JID - 101676056
PMC - PMC10045139
OTO - NOTNLM
OT  - E11 podoplanin
OT  - LIPUS
OT  - MLO-Y4
OT  - apoptosis
OT  - bone regeneration
OT  - connectivity
OT  - connexin 43
OT  - mechanical stimulation
OT  - osteocytes
OT  - ultrasound
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:01
PMCR- 2023/03/21
CRDT- 2023/03/29 01:16
PHST- 2023/01/31 00:00 [received]
PHST- 2023/03/01 00:00 [revised]
PHST- 2023/03/15 00:00 [accepted]
PHST- 2023/03/30 06:01 [medline]
PHST- 2023/03/29 01:16 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/21 00:00 [pmc-release]
AID - bioengineering10030387 [pii]
AID - bioengineering-10-00387 [pii]
AID - 10.3390/bioengineering10030387 [doi]
PST - epublish
SO  - Bioengineering (Basel). 2023 Mar 21;10(3):387. doi: 
      10.3390/bioengineering10030387.