PMID- 36979618
OWN - NLM
STAT- MEDLINE
DCOM- 20230330
LR  - 20230331
IS  - 2079-6374 (Electronic)
IS  - 2079-6374 (Linking)
VI  - 13
IP  - 3
DP  - 2023 Mar 20
TI  - Clinically Deployable Bioelectronic Sensing Platform for Ultrasensitive Detection 
      of Transferrin in Serum Sample.
LID - 10.3390/bios13030406 [doi]
LID - 406
AB  - Varying levels of transferrin (Tf) have been associated with different disease 
      conditions and are known to play a crucial role in various malignancies. Regular 
      monitoring of the variations in Tf levels can be useful for managing related 
      diseases, especially for the prognosis of certain cancers. We fabricated an 
      immunosensor based on graphene oxide (GO) nanosheets to indirectly detect Tf 
      levels in cancer patients. The GO nanosheets were deposited onto an indium tin 
      oxide (ITO)-coated glass substrate and annealed at 120  degrees C to obtain reduced GO 
      (rGO) films, followed by the immobilization of an antibody, anti-Tf. The 
      materials and sensor probe used were systematically characterized by UV-Visible 
      spectroscopy (UV-Vis), X-ray diffraction (XRD), atomic force microscopy (AFM), 
      and Fourier transform infrared spectroscopy (FTIR). Cyclic voltammetry (CV), 
      electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry 
      (DPV) were also used for the stepwise sensor probe characterizations and Tf 
      detection in serum samples, respectively. The anti-Tf/rGO/ITO immunosensor DPV 
      output demonstrated an excellent Tf detection capability in the linear range of 
      0.1 mg mL(-1) to 12 mg mL(-1) compared to the enzyme-linked immunosorbent assay 
      (ELISA) detection range, with a limit of detection (LOD) of 0.010 +/- 0.007 mg 
      mL(-1). Furthermore, the results of the fabricated immunosensor were compared 
      with those of the ELISA and autobioanalyzer techniques, showing an outstanding 
      match with < 5% error and demonstrating the immunosensor's clinical potential.
FAU - Kaur, Harleen
AU  - Kaur H
AD  - CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, 
      India.
FAU - Chittineedi, Prasanthi
AU  - Chittineedi P
AD  - Onco-Stem Cell Research Laboratory, Department of Biochemistry and 
      Bioinformatics, GITAM School of Science, GITAM (Deemed to be) University, 
      Visakhapatnam 530045, India.
FAU - Bellala, Ravi Shankar
AU  - Bellala RS
AD  - Department of Clinical Oncology, Omega Hospitals, Visakhapatnam 530040, India.
FAU - Bellala, Venkata Madhavi
AU  - Bellala VM
AD  - Department of Pathology, GITAM Institute of Medical Sciences and Research, 
      Visakhapatnam 530045, India.
FAU - Singh, Sandeep
AU  - Singh S
AD  - CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, 
      India.
FAU - Kumari, Rohini
AU  - Kumari R
AD  - Laboratory of Bio-Physio Sensors and Nanobioengineering, School of Biochemical 
      Engineering, Indian Institute of Technology (BHU), Varanasi 221005, India.
FAU - Chandra, Pranjal
AU  - Chandra P
AUID- ORCID: 0000-0003-3428-3195
AD  - Laboratory of Bio-Physio Sensors and Nanobioengineering, School of Biochemical 
      Engineering, Indian Institute of Technology (BHU), Varanasi 221005, India.
FAU - Pandrangi, Santhi Latha
AU  - Pandrangi SL
AUID- ORCID: 0000-0001-5317-8716
AD  - Onco-Stem Cell Research Laboratory, Department of Biochemistry and 
      Bioinformatics, GITAM School of Science, GITAM (Deemed to be) University, 
      Visakhapatnam 530045, India.
FAU - Singh, Surinder P
AU  - Singh SP
AD  - CSIR-National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110012, 
      India.
AD  - Academy of Scientific and Innovative Research (AcSIR), CSIR-HRDC Campus, 
      Ghaziabad 201002, India.
LA  - eng
PT  - Journal Article
DEP - 20230320
PL  - Switzerland
TA  - Biosensors (Basel)
JT  - Biosensors
JID - 101609191
RN  - 0 (graphene oxide)
RN  - 0 (Transferrin)
RN  - 7782-42-5 (Graphite)
RN  - 71243-84-0 (indium tin oxide)
SB  - IM
MH  - Humans
MH  - Immunoassay/methods
MH  - *Biosensing Techniques/methods
MH  - Transferrin
MH  - Electrochemical Techniques
MH  - *Neoplasms
MH  - *Graphite/chemistry
MH  - Limit of Detection
PMC - PMC10046405
OTO - NOTNLM
OT  - electrochemical detection
OT  - ferritin
OT  - immunosensors
OT  - reduced graphene oxide
OT  - transferrin
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:11
PMCR- 2023/03/20
CRDT- 2023/03/29 01:20
PHST- 2023/02/09 00:00 [received]
PHST- 2023/03/16 00:00 [revised]
PHST- 2023/03/18 00:00 [accepted]
PHST- 2023/03/30 06:11 [medline]
PHST- 2023/03/29 01:20 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/20 00:00 [pmc-release]
AID - bios13030406 [pii]
AID - biosensors-13-00406 [pii]
AID - 10.3390/bios13030406 [doi]
PST - epublish
SO  - Biosensors (Basel). 2023 Mar 20;13(3):406. doi: 10.3390/bios13030406.