PMID- 36979734
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230331
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 11
IP  - 3
DP  - 2023 Mar 2
TI  - Fluorescence In Situ Hybridization in Primary Diagnosis of Biliary Strictures: A 
      Single-Center Prospective Interventional Study.
LID - 10.3390/biomedicines11030755 [doi]
LID - 755
AB  - Background and aims: Diagnosis of the biliary stricture remains a challenge. In 
      view of the low sensitivity of brush cytology (BC), fluorescence in situ 
      hybridization (FISH) has been reported as a useful adjunctive test in patients 
      with biliary strictures. We aimed to determine performance characteristics of BC 
      and FISH individually and in combination (BC + FISH) in the primary diagnosis of 
      biliary strictures. Methods: This single-center prospective study was conducted 
      between April 2019 and January 2021. Consecutive patients with unsampled biliary 
      strictures undergoing first endoscopic retrograde cholangiopancreatography in our 
      institution were included. Tissue specimens from two standardized transpapillary 
      brushings from the strictures were examined by routine cytology and FISH. 
      Histopathological confirmation after surgery or 12-month follow-up was regarded 
      as the reference standard for final diagnosis. Results: Of 109 enrolled patients, 
      six were excluded and one lost from the final analysis. In the remaining 102 
      patients (60.8% males, mean age 67.4, range 25-92 years), the proportions of 
      benign and malignant strictures were 28 (27.5%) and 74 (72.5%), respectively. The 
      proportions of proximal and distal strictures were 26 (25.5%) and 76 (74.5%), 
      respectively. In comparison to BC alone, FISH increased the sensitivity from 
      36.1% to 50.7% (p = 0.076) while maintaining similar specificity (p = 0.311). 
      Conclusions: Dual-modality tissue evaluation using BC + FISH showed an improving 
      trend in sensitivity for the primary diagnosis of biliary strictures when 
      compared with BC alone.
FAU - Zoundjiekpon, Vincent Dansou
AU  - Zoundjiekpon VD
AUID- ORCID: 0000-0002-5673-3276
AD  - 2nd Department of Internal Medicine, Gastroenterology and Geriatrics, University 
      Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University Olomouc, 
      779 00 Olomouc, Czech Republic.
FAU - Falt, Premysl
AU  - Falt P
AD  - 2nd Department of Internal Medicine, Gastroenterology and Geriatrics, University 
      Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University Olomouc, 
      779 00 Olomouc, Czech Republic.
FAU - Zapletalova, Jana
AU  - Zapletalova J
AD  - Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky 
      University Olomouc, 779 00 Olomouc, Czech Republic.
FAU - Vanek, Petr
AU  - Vanek P
AUID- ORCID: 0000-0001-9573-5277
AD  - 2nd Department of Internal Medicine, Gastroenterology and Geriatrics, University 
      Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University Olomouc, 
      779 00 Olomouc, Czech Republic.
FAU - Kurfurstova, Daniela
AU  - Kurfurstova D
AUID- ORCID: 0000-0002-3175-3131
AD  - Institute of Clinical and Molecular Pathology, University Hospital Olomouc, 
      Faculty of Medicine and Dentistry, Palacky University Olomouc, 779 00 Olomouc, 
      Czech Republic.
FAU - Slobodova, Zuzana
AU  - Slobodova Z
AD  - Institute of Clinical and Molecular Pathology, University Hospital Olomouc, 
      Faculty of Medicine and Dentistry, Palacky University Olomouc, 779 00 Olomouc, 
      Czech Republic.
FAU - Skanderova, Daniela
AU  - Skanderova D
AD  - Institute of Clinical and Molecular Pathology, University Hospital Olomouc, 
      Faculty of Medicine and Dentistry, Palacky University Olomouc, 779 00 Olomouc, 
      Czech Republic.
FAU - Korinkova, Gabriela
AU  - Korinkova G
AD  - Institute of Clinical and Molecular Pathology, University Hospital Olomouc, 
      Faculty of Medicine and Dentistry, Palacky University Olomouc, 779 00 Olomouc, 
      Czech Republic.
FAU - Skalicky, Pavel
AU  - Skalicky P
AD  - Department of Surgery I, University Hospital Olomouc, Faculty of Medicine and 
      Dentistry, Palacky University Olomouc, 779 00 Olomouc, Czech Republic.
FAU - Lovecek, Martin
AU  - Lovecek M
AUID- ORCID: 0000-0001-9775-9643
AD  - Department of Surgery I, University Hospital Olomouc, Faculty of Medicine and 
      Dentistry, Palacky University Olomouc, 779 00 Olomouc, Czech Republic.
FAU - Urban, Ondrej
AU  - Urban O
AUID- ORCID: 0000-0002-6705-7912
AD  - 2nd Department of Internal Medicine, Gastroenterology and Geriatrics, University 
      Hospital Olomouc, Faculty of Medicine and Dentistry, Palacky University Olomouc, 
      779 00 Olomouc, Czech Republic.
LA  - eng
GR  - DRO 87-96 (FNOL, 00098892)/supported by the grant MH CZ -/
PT  - Journal Article
DEP - 20230302
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC10045065
OTO - NOTNLM
OT  - ZytoLight FISH probe
OT  - brush cytology
OT  - first retrograde cholangiopancreatography
OT  - fluorescence in situ hybridization
OT  - primary diagnosis of biliary strictures
COIS- The authors declare that there is no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:01
PMCR- 2023/03/02
CRDT- 2023/03/29 01:20
PHST- 2023/01/28 00:00 [received]
PHST- 2023/02/22 00:00 [revised]
PHST- 2023/02/24 00:00 [accepted]
PHST- 2023/03/30 06:01 [medline]
PHST- 2023/03/29 01:20 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/02 00:00 [pmc-release]
AID - biomedicines11030755 [pii]
AID - biomedicines-11-00755 [pii]
AID - 10.3390/biomedicines11030755 [doi]
PST - epublish
SO  - Biomedicines. 2023 Mar 2;11(3):755. doi: 10.3390/biomedicines11030755.