PMID- 36980362
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230331
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 13
IP  - 6
DP  - 2023 Mar 10
TI  - Microbial Sampling Using Interdental Brushes and Paper Points around Teeth and 
      Implants: A Pilot Study for Comparison.
LID - 10.3390/diagnostics13061054 [doi]
LID - 1054
AB  - Inflammatory periodontal and peri-implant diseases follow dysbiotic shifts in a 
      susceptible host. A well-established tool for microbial sample collection is the 
      use of paper points. The purpose of this pilot study was to evaluate the use of 
      interdental brushes compared to paper points. Biofilm samples were collected with 
      paper points and later interdental brushes from ten patients. Five patients were 
      represented with a community periodontal index of treatment needs (CPITN) of 0-2 
      around the teeth and an implant with PPD </= 5 mm and no radiological bone loss. 
      The remaining five patients had a CPITN >/= 3 and one implant with 
      peri-implantitis. Microbial samples were analyzed with quantitative polymerase 
      chain reaction (qPCR) and next-generation sequencing (NGS). The results showed 
      higher amounts of DNA in samples taken by interdental brushes but also higher Ct 
      values. Both methods detected Filifactor alocis, Porphyromonas gingivalis, 
      Prevotella intermedia, Tannerella forsythia, and Treponema denticola in the 
      majority of samples, while Aggregatibacter actinomycetemcomitans was rarely 
      found. A microbial dysbiosis index showed comparable or higher values in sites 
      with no periodontitis/peri-implantitis with interdental brushes. The results of 
      this pilot study indicate that interdental brushes might be a valid technique for 
      microbial sampling and particularly advantageous in the early detection of 
      dysbiotic shifts around teeth and implants. Larger studies with more participants 
      are needed to validate the proposed microbial sampling method with interdental 
      brushes.
FAU - Janson, Tobias M
AU  - Janson TM
AD  - Clinic of Conservative & Preventive Dentistry, Division of Periodontology & 
      Peri-Implant Diseases, Center of Dental Medicine, University of Zurich, 8032 
      Zurich, Switzerland.
FAU - Gager, Yann
AU  - Gager Y
AD  - ParoX Dental Gmbh, 04103 Leipzig, Germany.
FAU - Hatz, Christian R
AU  - Hatz CR
AUID- ORCID: 0000-0003-3317-0770
AD  - Clinic of Conservative & Preventive Dentistry, Division of Periodontology & 
      Peri-Implant Diseases, Center of Dental Medicine, University of Zurich, 8032 
      Zurich, Switzerland.
FAU - Kohler, Anne-Katrin
AU  - Kohler AK
AD  - ParoX Dental Gmbh, 04103 Leipzig, Germany.
FAU - Gartenmann, Stefanie J
AU  - Gartenmann SJ
AD  - Dr. Stefanie Gartenmann Dental Practice, 6330 Cham, Switzerland.
FAU - Schmidlin, Patrick R
AU  - Schmidlin PR
AUID- ORCID: 0000-0002-1377-0325
AD  - Clinic of Conservative & Preventive Dentistry, Division of Periodontology & 
      Peri-Implant Diseases, Center of Dental Medicine, University of Zurich, 8032 
      Zurich, Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20230310
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC10047167
OTO - NOTNLM
OT  - interdental cleaning
OT  - microbial sampling
OT  - molecular genetics
OT  - peri-implantitis
OT  - periodontitis
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:01
PMCR- 2023/03/10
CRDT- 2023/03/29 01:24
PHST- 2023/02/08 00:00 [received]
PHST- 2023/03/06 00:00 [revised]
PHST- 2023/03/06 00:00 [accepted]
PHST- 2023/03/30 06:01 [medline]
PHST- 2023/03/29 01:24 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/10 00:00 [pmc-release]
AID - diagnostics13061054 [pii]
AID - diagnostics-13-01054 [pii]
AID - 10.3390/diagnostics13061054 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2023 Mar 10;13(6):1054. doi: 10.3390/diagnostics13061054.