PMID- 36982813
OWN - NLM
STAT- MEDLINE
DCOM- 20230330
LR  - 20230331
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 6
DP  - 2023 Mar 17
TI  - Asprosin Enhances Cytokine Production by a Co-Culture of Fully Differentiated 
      Mature Adipocytes and Macrophages Leading to the Exacerbation of the Condition 
      Typical of Obesity-Related Inflammation.
LID - 10.3390/ijms24065745 [doi]
LID - 5745
AB  - Asprosin, a fasting-induced, glucogenic, and orexigenic adipokine, has gained 
      popularity in recent years as a potential target in the fight against obesity and 
      its complications. However, the contribution of asprosin to the development of 
      moderate obesity-related inflammation remains still unknown. The present study 
      aimed to evaluate the effect of asprosin on the inflammatory activation of 
      adipocyte-macrophage co-cultures at various stages of differentiation. The study 
      was performed on co-cultures of the murine 3T3L1 adipocyte and the RAW264.7 
      macrophage cell lines treated with asprosin before, during, and after 3T3L1 cell 
      differentiation, with or without lipopolysaccharide (LPS) stimulation. Cell 
      viability, overall cell activity, and the expression and release of key 
      inflammatory cytokines were analyzed. In the concentration range of 50-100 nM, 
      asprosin increased the pro-inflammatory activity in the mature co-culture and 
      enhanced the expression and release of tumor necrosis factor alpha (TNF-alpha), 
      high-mobility group box protein 1 (HMGB1), and interleukin 6 (IL-6). Macrophage 
      migration was also increased, which could be related to the upregulated 
      expression and release of monocyte chemoattractant protein-1 (MCP-1) by the 
      adipocytes. In summary, asprosin exerted a pro-inflammatory effect on the mature 
      adipocyte-macrophage co-culture and may contribute to the spread of moderate 
      obesity-associated inflammation. Nevertheless, further research is needed to 
      fully elucidate this process.
FAU - Mazur-Bialy, Agnieszka I
AU  - Mazur-Bialy AI
AUID- ORCID: 0000-0003-1056-8276
AD  - Department of Biomechanics and Kinesiology, Faculty of Health Science, 
      Jagiellonian University Medical College, Skawinska 8, 31-066 Krakow, Poland.
LA  - eng
GR  - N43/DBS/000184/Jagiellonian University/
PT  - Journal Article
DEP - 20230317
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Coculture Techniques
MH  - *Obesity/metabolism
MH  - *Inflammation/metabolism
MH  - Adipocytes/metabolism
MH  - Cytokines/metabolism
MH  - Macrophages/metabolism
MH  - Cell Differentiation
MH  - 3T3-L1 Cells
PMC - PMC10056564
OTO - NOTNLM
OT  - adipocytes
OT  - asprosin
OT  - inflammation
OT  - macrophages
OT  - obesity
OT  - obesity-related inflammation
COIS- The author declares no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:11
PMCR- 2023/03/17
CRDT- 2023/03/29 01:39
PHST- 2023/02/04 00:00 [received]
PHST- 2023/03/09 00:00 [revised]
PHST- 2023/03/14 00:00 [accepted]
PHST- 2023/03/30 06:11 [medline]
PHST- 2023/03/29 01:39 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/03/17 00:00 [pmc-release]
AID - ijms24065745 [pii]
AID - ijms-24-05745 [pii]
AID - 10.3390/ijms24065745 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Mar 17;24(6):5745. doi: 10.3390/ijms24065745.