PMID- 36984803
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230331
IS  - 2218-1989 (Print)
IS  - 2218-1989 (Electronic)
IS  - 2218-1989 (Linking)
VI  - 13
IP  - 3
DP  - 2023 Feb 28
TI  - Exposure to Particulate Matter in the Broiler House Causes Dyslipidemia and 
      Exacerbates It by Damaging Lung Tissue in Broilers.
LID - 10.3390/metabo13030363 [doi]
LID - 363
AB  - The high concentration of particulate matter (PM) in broiler houses seriously 
      endangers the biological safety of broilers and causes low growth performance, 
      deserving more attention. This study aimed to investigate the effects of PM 
      collected from a broiler house on the lung and systemic inflammatory responses 
      and liver lipid anabolic process in broilers. Broilers were systemically exposed 
      to fresh air (control) and 4 mg.m(-3) and 8 mg.m(-3) total suspended particles 
      (TSP). Lung, liver, and serum were sampled after 7 (E7) and 14 (E14) days of PM 
      exposure and 7 days after self-recovery (R 7). Corresponding kits were used to 
      assay the inflammatory cytokines and serum biochemical indicators. The expression 
      levels of genes related to lipid metabolism were detected by real-time polymerase 
      chain reaction (RT-PCR) assay. The results showed a significant decrease in the 
      average daily gain in broilers for 7 days of PM exposure (p < 0.05) and clear 
      lung and liver inflammations in PM groups. In addition, upregulation of lung 
      interleukin (IL)-1beta and IL-8 and serum low-density lipoprotein cholesterol 
      (LDL-C) and triglyceride (TG) occurred after 7 days of PM exposure (p < 0.05), 
      and upregulation of lung serum tumor necrosis factor (TNF)-alpha and cholesterol 
      (CHOL) occurred after 14 days of PM exposure (p < 0.05). A decrease in serum 
      total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-px) levels was 
      found after 14 days of PM exposure (p < 0.05), and the GSH-px level was 
      maintained until 7 days after cessation of exposure (p < 0.05). Seven days after 
      cessation of exposure, the expression levels of 3-hydroxy-3-methylglutaryl-CoA 
      synthase 2 (Hmgcs2) and fatty acid synthase (Fas) genes significantly increased 
      (p < 0.05) and decreased (p < 0.05), respectively. These results demonstrate that 
      exposure to PM in broiler houses can induce systemic inflammation and 
      dyslipidemia through local pulmonary inflammation and also exert toxic effects on 
      the liver by disturbing the expression of genes involved in the hepatic lipid 
      anabolic process.
FAU - Shen, Dan
AU  - Shen D
AUID- ORCID: 0000-0001-8350-2804
AD  - Research Centre for Livestock Environmental Control and Smart Production, College 
      of Animal Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Guo, Qi
AU  - Guo Q
AD  - Research Centre for Livestock Environmental Control and Smart Production, College 
      of Animal Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Huang, Kai
AU  - Huang K
AD  - Research Centre for Livestock Environmental Control and Smart Production, College 
      of Animal Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Mao, Weijia
AU  - Mao W
AD  - Research Centre for Livestock Environmental Control and Smart Production, College 
      of Animal Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Wang, Kai
AU  - Wang K
AD  - Research Centre for Livestock Environmental Control and Smart Production, College 
      of Animal Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Zeng, Wenjie
AU  - Zeng W
AD  - Research Centre for Livestock Environmental Control and Smart Production, College 
      of Animal Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Li, Yansen
AU  - Li Y
AD  - Research Centre for Livestock Environmental Control and Smart Production, College 
      of Animal Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
FAU - Guo, Zhendong
AU  - Guo Z
AD  - Military Veterinary Research Institute, Academy of Military Medical Sciences, 
      Changchun 130117, China.
FAU - Nagaoka, Kentaro
AU  - Nagaoka K
AD  - Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty 
      of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, 
      Japan.
FAU - Li, Chunmei
AU  - Li C
AUID- ORCID: 0000-0002-6158-1254
AD  - Research Centre for Livestock Environmental Control and Smart Production, College 
      of Animal Science and Technology, Nanjing Agricultural University, Nanjing 
      210095, China.
LA  - eng
GR  - 32072781/National Natural Science Foundation of China/
GR  - 2022M721651/China Postdoctoral Science Foundation/
GR  - JATS(2022)480/Jiangsu Agricultural Industry Technology System/
PT  - Journal Article
DEP - 20230228
PL  - Switzerland
TA  - Metabolites
JT  - Metabolites
JID - 101578790
PMC - PMC10052321
OTO - NOTNLM
OT  - broiler house
OT  - dyslipidemia
OT  - inflammatory response
OT  - lung injury
OT  - particulate matter
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:01
PMCR- 2023/02/28
CRDT- 2023/03/29 01:51
PHST- 2023/02/07 00:00 [received]
PHST- 2023/02/21 00:00 [revised]
PHST- 2023/02/24 00:00 [accepted]
PHST- 2023/03/30 06:01 [medline]
PHST- 2023/03/29 01:51 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/02/28 00:00 [pmc-release]
AID - metabo13030363 [pii]
AID - metabolites-13-00363 [pii]
AID - 10.3390/metabo13030363 [doi]
PST - epublish
SO  - Metabolites. 2023 Feb 28;13(3):363. doi: 10.3390/metabo13030363.