PMID- 36985136
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230331
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 11
IP  - 3
DP  - 2023 Feb 23
TI  - Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in 
      Paracoccidioidomycosis Patient Tissue Samples.
LID - 10.3390/microorganisms11030562 [doi]
LID - 562
AB  - Paracoccidioidomycosis (PCM) is the main and most prevalent systemic mycosis in 
      Latin America, that until recently, it was believed to be caused only by 
      Paracoccidioides brasiliensis (P. brasiliensis). In 2006, researchers described 
      three cryptic species: S1, PS2, PS3, and later, another one, PS4. In 2009, 
      Paracoccidioides lutzii (Pb01-like) was described, and in 2017, a new 
      nomenclature was proposed for the different agents: P. brasiliensis (S1), P. 
      americana (PS2), P. restrepiensis (PS3), and P. venezuelensis (PS4). These 
      species are not uniformly distributed throughout Latin America and, knowing that 
      more than one cryptic species could coexist in some regions, we aimed to identify 
      those species in patients' biopsy samples for a better understanding of the 
      distribution and occurrence of these recently described species in Botucatu 
      region. The Hospital of Medical School of Botucatu-UNESP, which is a PCM study 
      pole, is located in Sao Paulo State mid-west region and is classified as a PCM 
      endemic area. Genotyping analyses of clinical specimens from these patients that 
      have been diagnosed and treated in our Hospital could favor a possible 
      correlation between genetic groups and mycological and clinical characteristics. 
      For this, molecular techniques to differentiate Paracoccidioides species in these 
      biopsies, such as DNA extraction, PCR, and sequencing of three target genes (ITS, 
      CHS2, and ARF) were conducted. All the sequences were analyzed at BLAST to 
      testify the presence of P. brasiliensis. The phylogenetic trees were constructed 
      using Mega 7.0 software and showed that 100% of our positive samples were from S1 
      cryptic species, therefore P. brasiliensis. This is important data, demonstrating 
      the predominance of this species in the Sao Paulo State region.
FAU - de Oliveira, Luciana Bonome Zeminian
AU  - de Oliveira LBZ
AD  - Laboratory of Immunopathology and Infectious Agents-LIAI, UNIPEX-Experimental 
      Research Unity-Sector 5, Medical School of Botucatu, Sao Paulo State University 
      (UNESP), Botucatu 18618-687, SP, Brazil.
FAU - Della Coletta, Amanda Manoel
AU  - Della Coletta AM
AD  - Laboratory of Immunopathology and Infectious Agents-LIAI, UNIPEX-Experimental 
      Research Unity-Sector 5, Medical School of Botucatu, Sao Paulo State University 
      (UNESP), Botucatu 18618-687, SP, Brazil.
FAU - Gardizani, Taiane Priscila
AU  - Gardizani TP
AD  - Laboratory of Immunopathology and Infectious Agents-LIAI, UNIPEX-Experimental 
      Research Unity-Sector 5, Medical School of Botucatu, Sao Paulo State University 
      (UNESP), Botucatu 18618-687, SP, Brazil.
FAU - Garces, Hans Garcia
AU  - Garces HG
AUID- ORCID: 0000-0002-6100-3534
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil.
FAU - Bagagli, Eduardo
AU  - Bagagli E
AUID- ORCID: 0000-0002-8003-4109
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University (UNESP), Botucatu 18618-689, SP, Brazil.
FAU - Trilles, Luciana
AU  - Trilles L
AD  - Evandro Chagas National Institute of Infectious Disease, Oswaldo Cruz Foundation 
      (FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil.
FAU - Barrozo, Ligia Vizeu
AU  - Barrozo LV
AUID- ORCID: 0000-0001-7322-6789
AD  - Department of Geography, School of Philosophy, Literature and Human Sciences, 
      University of Sao Paulo (USP), Sao Paulo 05508-080, SP, Brazil.
FAU - Marques, Silvio de Alencar
AU  - Marques SA
AD  - Department of Dermatology, Medical School of Botucatu, Sao Paulo State University 
      (UNESP), Botucatu 18618-687, SP, Brazil.
FAU - Faveri, Julio De
AU  - Faveri J
AD  - Department of Pathology, Medical School of Botucatu, Sao Paulo State University 
      (UNESP), Botucatu 18618-687, SP, Brazil.
FAU - Dias-Melicio, Luciane Alarcao
AU  - Dias-Melicio LA
AUID- ORCID: 0000-0001-9254-2074
AD  - Laboratory of Immunopathology and Infectious Agents-LIAI, UNIPEX-Experimental 
      Research Unity-Sector 5, Medical School of Botucatu, Sao Paulo State University 
      (UNESP), Botucatu 18618-687, SP, Brazil.
AD  - Department of Pathology, Medical School of Botucatu, Sao Paulo State University 
      (UNESP), Botucatu 18618-687, SP, Brazil.
LA  - eng
GR  - FAPESP 2013/24877-9/Sao Paulo Research Foundation/
PT  - Journal Article
DEP - 20230223
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10055015
OTO - NOTNLM
OT  - Paracoccidioides brasiliensis
OT  - Paracoccidioides species complex
OT  - molecular biology
OT  - molecular phylogenetic analysis
OT  - paracoccidiodomycosis
COIS- The authors declare no conflict of interest.
EDAT- 2023/03/30 06:00
MHDA- 2023/03/30 06:01
PMCR- 2023/02/23
CRDT- 2023/03/29 01:53
PHST- 2022/11/30 00:00 [received]
PHST- 2023/02/10 00:00 [revised]
PHST- 2023/02/15 00:00 [accepted]
PHST- 2023/03/30 06:01 [medline]
PHST- 2023/03/29 01:53 [entrez]
PHST- 2023/03/30 06:00 [pubmed]
PHST- 2023/02/23 00:00 [pmc-release]
AID - microorganisms11030562 [pii]
AID - microorganisms-11-00562 [pii]
AID - 10.3390/microorganisms11030562 [doi]
PST - epublish
SO  - Microorganisms. 2023 Feb 23;11(3):562. doi: 10.3390/microorganisms11030562.